Labshake search
Citations for Stemcell Technologies :
151 - 200 of 1567 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
-
bioRxiv - Neuroscience 2019Quote: ... Human splenocytes were then cultured at 1×106 cell per mL in ImmunoCult-XF T cell Expansion Medium (Stemcell Technologies, Vancouver, BC). For T cell stimulation ...
-
bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
-
bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
-
bioRxiv - Immunology 2019Quote: ... Primary human NK cells were isolated from leukocyte-enriched peripheral blood of healthy human donors using the RosetteSep™ Human NK Cell Enrichment Cocktail (StemCell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Human NK were isolated from discarded Leukopaks from healthy volunteer blood donors using the RosetteSep™ human NK enrichment protocol (StemCell Technologies), followed by Ficoll (GE Healthcare ...
-
bioRxiv - Immunology 2022Quote: ... Naïve human CD4+ T cells were isolated using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
-
bioRxiv - Immunology 2020Quote: ... Human T cells were also isolated from the thymus tissue in humanized SRG rats via immunomagnetic selection using anti-human CD3 antibody (EasySep™ Human CD3 Positive Selection, Catalog # 17951, Stemcell Technologies) and treated without (vehicle ...
-
bioRxiv - Cell Biology 2023Quote: Primary human CD4+ T cells were isolated from leukocyte cones using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). T cells were cultured at 37°C and 5% CO2 in RPMI 1640 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... These GBM cell lines were maintained as BTICs in human neural stem cell (NSC) media containing NeuroCult NS-A basal medium (human, STEMCELL Technologies, #05750), supplemented NeuroCult NS-A proliferation kit (human ...
-
bioRxiv - Microbiology 2023Quote: ... Human CD34+ cells were isolated from cord blood using EasySep™ human cord blood CD34 positive selection kit II (STEMCELL Technologies #17896), which were then cryopreserved in Iscove’s Modified Dulbecco’s Medium (IMDM ...
-
bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
-
bioRxiv - Immunology 2023Quote: ... Human blood neutrophils from healthy controls were isolated using negative sorting with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies 19666), loaded with DCFDA ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from human peripheral blood using EasySepTM Human CD8+ T Cell Isolation Kit (Stemcell Technologies, Vancouver, BC, Canada) and stained with 10 μM CellTraceTM Violet Cell Proliferation Kit (VPD ...
-
bioRxiv - Immunology 2024Quote: Human T cells were isolated from healthy donors who provided informed consent using RosetteSep Human T-Cell Enrichment Cocktail (STEMCELL Technologies, Canada) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... The plates were incubated at 37°C for 20 min to allow the domes to solidify after which 750 μl of human intestinal organoid growth media (Human IntestiCult Organoid Growth Medium, hIOGM, StemCell Technologies, 06010) was added along with 10 μM ROCK inhibitor (Y-27632 ...
-
bioRxiv - Microbiology 2019Quote: ... or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies) and then isolated with Ficoll-Paque Plus (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
-
bioRxiv - Cancer Biology 2019Quote: ... a Human Peripheral Blood Leuko Pak (Stemcell Technologies #70500.1) from an anonymous donor was diluted with an equivalent volume of PBS + 2% FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant IL-6 (STEMCELL Technologies) and 10 ng/ml mouse recombinant IL-3 (R&D ...
-
bioRxiv - Cancer Biology 2022Quote: RosetteSep™ Human Monocyte Enrichment Cocktail (Stemcell Technologies, Canada) was used to purify human monocytes from buffy coats following manufacturer specifications ...
-
bioRxiv - Pathology 2022Quote: Human biopsies were placed in PneumaCult medium (Stemcell Technologies) and sent for infection immediately ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human HSPCs were expanded in StemSpan SFEM (StemCell Technologies) with 1% streptomycin/penicillin ...
-
bioRxiv - Cell Biology 2020Quote: Human CB CD34+ cells were purchased from STEMCELL Technologies GmbH (Cologne ...
-
bioRxiv - Biochemistry 2019Quote: Human peripheral blood plasma (STEMCELL Technologies, cat. no. 70039) and plasma and bone marrow interstitial fluid samples from three pediatric B-ALL patients (B-ALL-1 ...
-
bioRxiv - Immunology 2020Quote: ... and the EasySep human monocyte isolation kit (StemCell Technologies) with equivalent results ...
-
bioRxiv - Biochemistry 2022Quote: ... with EasySep Human T cell Isolation Kit (STEMCELL Technologies). Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies) ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1000 U recombinant human LIF (StemCell Technologies #78055). Medium was replaced daily ...
-
bioRxiv - Cancer Biology 2023Quote: ... and CD28 (ImmunoCult human T cell activator, StemCell Technologies) in total volume of 200 μl complete RPMI medium in a 96-well U bottom plate in the presence of either DMSO or soquelitinib for 48 hours ...
-
bioRxiv - Immunology 2023Quote: Human PBMCs were obtained from Stemcell Technologies (Vancouver, BC). Samples were collected from donors and anonymized prior to providing for research purposes ...
-
bioRxiv - Microbiology 2023Quote: ... The RosetteSep™ Human Monocyte Enrichment Cocktail (STEMCELL Technologies) was used to purify monocytes from the PBMCs ...
-
bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (20 ng/mL, STEMCELL Technologies, #78006.2), human recombinant FGF (10 ng/mL ...
-
bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (2 ng/mL, STEMCELL Technologies, #78006.2), antibiotic-antimycotic (1X ...
-
bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (20 ng/mL, STEMCELL Technologies, #78006.2), human recombinant FGF (10 ng/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... and analyzed by STEMvision™ Human (Stem Cell Technologies).
-
bioRxiv - Immunology 2023Quote: Frozen human PBMCs from 5 healthy donors (Stemcell Technologies, Cat# 70025.2 ...
-
bioRxiv - Immunology 2024Quote: ... or Human T Cell Enrichment Kits (Stem Cell Technologies) and cultured in RPMI 1640 (ATCC ...
-
bioRxiv - Genomics 2024Quote: Human iPSCs were maintained in mTeSR1 (StemCell Tech, 85850) or mTeSR Plus (StemCell Tech ...
-
bioRxiv - Immunology 2024Quote: ... 1.5 µg/mL anti-human CD32 antibody (STEMCELL Technologies) and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed ...
-
bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purchased from StemCell Technologies. Viability was assessed via DAPI staining ...
-
bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
-
bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
-
bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
-
bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
-
bioRxiv - Immunology 2021Quote: Assays were performed on purified human classical monocytes isolated using immunomagnetic negative sorting (EasySep Direct Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, MA). As we have previously described 88 ...
-
bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
-
bioRxiv - Cell Biology 2023Quote: ... The middle layer containing PBMCs were then enriched for primary human NK cells (hNK) by negative selection using the EasySep Human NK Cell Enrichment Kit (StemCell Technologies, Cat# 19055), following the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... with the addition of ImmunoCult Human CD3/CD28/CD2 T Cell Activator and Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA) as per STEMCELL Technologies Inc ...