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Citations for Stemcell Technologies :
151 - 200 of 1170 citations for 6 Phenyl 1H imidazo 1 2 b pyrazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Primary mammary epithelial cells were cultured in EpiCult-B mouse medium (STEMCELL Technologies) supplemented with proliferation supplements ...
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bioRxiv - Immunology 2022Quote: ... and naïve B cells were isolated by negative selection (StemCell Technology, Catalog #19254). B cells were seeded in 24 well flat-bottom plates at a density of 1 × 105 per well and stimulated in 1 ml of B cells complete medium ...
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bioRxiv - Immunology 2022Quote: ... Splenic B cells were enriched with the EasySep kit (STEMCELL Technologies, Cambridge, MA). Bone marrow cells were obtained by flushing femora and tibiae with RPMI medium ...
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bioRxiv - Immunology 2022Quote: ... for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 7 days as described previously (41) ...
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bioRxiv - Cell Biology 2021Quote: ... H9-hESC SOX10::GFP reporter and H20961 iPSC were passaged ∼1:6 every 4-5 days by incubating them in ReLeSR (StemCell technologies) for 1 min at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... and cultured at a concentration of 1×103 cells mL−1 in one well of a 6-well plate (#1008; Becton-Dickinson) with 1 mL MethoCult GF+ semisolid medium (#4435, STEMCELL Technologies). Colonies were counted after 14 days of incubation using an inverted microscope by three individuals.
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies). After 14 days ...
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bioRxiv - Immunology 2021Quote: Murine T-cells were isolated from spleens of healthy 6-8-week old C57BL/6 mice using EasySep™ Mouse CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2019Quote: CD4+ T-cells were isolated from splenocytes and lymph node cells of wild type C57Bl/6 mice (6-8weeks old) using a T-cell negative selection kit (Easy Sep CD4+ T-cell enrichment kit, Stem Cell Technologies) to > 90% purity according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant IL-6 (STEMCELL Technologies) and 10 ng/ml mouse recombinant IL-3 (R&D ...
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bioRxiv - Microbiology 2020Quote: ... 6-well plates in mTeSR1 media (Stem Cell Technologies) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... with 10-6 M hydrocortisone (07904, Stem Cell Technologies), 1% penicillin streptomycin and 1ng/mL IL- 3 (R&D) ...
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bioRxiv - Bioengineering 2023Quote: ... and plated in a 6 well SmartDish (StemCell Technologies). CFU assays were quantified on day 14 using the STEMvision (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... in SmartDish meniscus-free 6-well plates (StemCell Technologies). Plates were kept in humidified chambers and colonies were imaged and manually scored after 9-14 days using the STEMvision counter (StemCell Technologies).
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 1x10-6 M hydrocortisone (StemCell Technologies, 74142), with half media exchanges twice per week.
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 1% N2 supplement and 2% NeuroCult S1 supplement (STEMCELL Technologies, Vancouver, BC). After one day media was removed and replaced with a thin layer of phosphate buffered saline ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 ng/mL FGF-2 (RnD Systems) and 1 µM CHIR99021 (Stem Cell Technologies) with daily medium exchange for 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 hiPSCs were cultured in mTeSR™1 medium (Stem Cell Technologies) on Matrigel (Corning)-coated plates following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... we enriched CD8+ T-cells from Cxcr3−/−/C57Bl/6 mice or non-transgenic C57Bl/6 mice to 80% purity using a negative selection method (Stemcell Technologies, Vancouver, Canada). Then ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies (Vancouver, Canada). Cells were thawed per manufacturer’s instructions and cultured as previously described.60 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.25 μg/mL amphotericin B) supplemented with 10 μM Y-27632 (STEMCELL Technologies). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of 2% rat serum (Stemcell technologies) was added to each well for 15 minutes at room temperature and proceeded to further staining without a washing step.
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow aspirates were diluted 1:1 with PBS (+ 2% FBS) and layered on a Lymphoprep gradient using SepMate tubes (STEMCELL Technologies). After centrifugation ...
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bioRxiv - Neuroscience 2022Quote: The iPSCs were obtained from the National Institutes of Health Cell Repositories and maintained on Matrigel (BD)-coated 6-well plates in mTeSR Plus medium (STEMCELL Technologies) using a feeder-free culture protocol ...
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bioRxiv - Microbiology 2020Quote: ... 6-well plates using mTeSR1 medium (Stem Cell Technologies, 83850). Neuronal differentiation was chemically induced by culturing hiPSC colonies in suspension in ultra-low attachment T75 flasks (Corning ...
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bioRxiv - Microbiology 2020Quote: ... coated 6-well plates in mTeSR1 media (Stem Cell Technologies) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10-6 M hydrocortisone (74142, Stem Cell Technologies) and 1% penicillin streptomycin ...
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bioRxiv - Developmental Biology 2023Quote: ... coated 6 well plates in mTeSR1 medium (StemCell Tech, 85850) at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coated 6-well plate in mTeSR1 (STEMCELL Technologies, cat # 85870) media and were passaged every 4 days at a ratio of 1:6 t o1:8 ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL recombinant IL-6 (StemCell Technologies cat. #78052.1) 3 ng/mL recombinant murine IL-1α (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... coated 6-well plates in mTESR1 medium (Stem Cell Technologies). Details on the cell lines used are available in Supplementary figures ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×103 cells were resuspended in 1 ml of complete MammoCultTM Basal Medium (Stemcell Tech) and seeded in ultralow attachment plates ...
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bioRxiv - Immunology 2021Quote: ... Isolation of T and B cells were performed according to the manufacturer’s protocols (Stemcell Technologies).
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bioRxiv - Pathology 2019Quote: ... iPSCs were exposed to N2 media containing 5 mL N2 Supplement-B (Stemcell Technologies 07156), 0.5mL 55mM β-mercaptoethanol (Life technologies 21985-023) ...
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with RPMI1640 enriched with 5ng/mL PDGF-B (Stem Cell Technologies), 2.5 ng/mL TGF-B (PeproTech) ...
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bioRxiv - Immunology 2022Quote: ... and human B-cell activating factor (BAFF) in 80 mL StemCell medium A (StemCell Technologies) containing 1 μg/mL of cyclosporine A (Millipore-Sigma) ...
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bioRxiv - Genomics 2020Quote: ... B lymphocytes and CD4+ T lymphocytes were isolated from PBMCs by negative selection (STEMCELL Technologies; cat ...
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bioRxiv - Immunology 2021Quote: ... and magnetic beads were used for negative selection of bulk B cells (StemCell Technologies, 19054) or naïve B cells (17254) ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole blood was diluted 1:1 with phosphate-buffered saline (PBS) containing 2% FBS and then layered onto Lymphoprep (STEMCELL Technologies, 07851) in SepMate-50 isolation tubes (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stemgent or Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Neuroscience 2021Quote: ... coated 6-well plates and maintained in mTeSR1 medium (STEMCELL Technologies). Nociceptor differentiation was performed as previously published (18 ...
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bioRxiv - Neuroscience 2022Quote: ... 6-well plate for 14 days using Brainphys (Stem Cell Technologies) 1X B27 Supplement (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... and passaged once every 5-6 days using Dispase (StemCell Technology). To maintain pluripotency and limit spontaneous differentiation ...