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Citations for Stemcell Technologies :
151 - 200 of 323 citations for 3 22 Dihydroxyolean 12 En 29 Oic Acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... isolated glands were minced and digested for 16-20 hr at 37 °C in DMEM F-12 (StemCell Technologies, 36254) containing 2% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... for 10 min at 37° C and seeded on to Geltrex-coated 12-well plates at a density of 700,000 cells in neural induction medium + 10 uM Y-27632 (Stemcell Technologies) and cultured for 7 days with daily medium exchange ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated into single-cell suspension using Accutase and re-seeded onto fresh Poly-L-Ornithine / Laminin coated 12-well plates at a density of 150,000 cells in STEMdiff Neuron Maturation Kit medium (Stemcell Technologies) and medium exchanged every other day until days 14 and 21.
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bioRxiv - Cancer Biology 2023Quote: ... Single HSCs (Lin-cKIT+ Sca1+ CD48-CD150+) were FACS-isolated and expanded for 12 days in StemSpan SFEM (Stemcell Technologies) containing 1% Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... and plated at 40 colonies/well on Matrigel-coated (0.08 mg/well) 12-well plates in mTeSR1 media (StemCell Technologies) supplemented with mTeSR1 5× Supplement (StemCell Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 days post-transfection in 1 ml STEMdiff Neural Progenitor Medium (Stemcell Technologies) cells were washed by DPBS and 2 wells of the plate were put together and collected by centrifugation at 10.000 rpm for 2 min generating 3 biological replicates ...
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bioRxiv - Bioengineering 2021Quote: ... single cells suspended in a 3:4 mixture of Mammocult (StemCell Technologies 05620) and Matrigel (Corning 354234 ...
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bioRxiv - Microbiology 2022Quote: ... and heat shock (3 hours at 43 □C) in BrainPhys (Stem Cell Technologies) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Neuroscience 2020Quote: ... This medium was supplemented with 3 μM CHIR99021 (STEMCELL Technologies; Cat. No. 72054), 2 μM DMH1 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 7 days as described previously (41) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 cells were then plated onto microwells (AggreWell 800, STEMCELL technologies) for embryoid body induction ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Physiology 2022Quote: ... cells were expanded for 3 days in StemSpan medium (Stem cell Technologies Inc) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were passaged 1:10 every 3 days using ReLeSR (StemCell Technologies #05873). Cells were maintained in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Systems Biology 2024Quote: ... we passaged the cells at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured cells in NPCs medium onwards.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured throughout on Matrigel substrates in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 with 15mM HEPES (DMEM/F12, STEMCELL Technologies, 36254) 1X Insulin Transferrin Selenium Liquid Media Supplement (ITS ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were cultured throughout on Matrigel substrates in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 with 15mM HEPES (DMEM/F12, STEMCELL Technologies, 36254) 1X Insulin Transferrin Selenium Liquid Media Supplement (ITS ...
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bioRxiv - Neuroscience 2021Quote: ... filled with cutting solution before transferring to individual wells in a 12 well plate filled with BrainPhys neuronal medium (StemCell Technologies) with the addition of the following supplements 2% (v/v ...
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bioRxiv - Cell Biology 2020Quote: Serum-free conditioned medium (CM) was essentially generated as described (8, 12, 13) by incubating serum-free medium (BIT 9500, STEMCELL Technologies) for three days on irradiated (30 Gy ...
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bioRxiv - Physiology 2023Quote: ... Cells were then plated between 450,000 - 750,000 cells per well of a Matrigel coated 12-well tissue culture plate in mTeSR1 PLUS™ (Stem Cell Technologies) supplemented with 10 µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Genomics 2023Quote: ... human iPSCs were plated at cell density of 3.5×105 cells/well on Matrigel coated 12-well-plates using mTeSR™ medium supplemented with 5µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then stimulated with 12 μL/mL ImmunoCult™(TM) Human CD3/CD28 T Cell Activator (STEMCELL Technologies, cat. #10971).
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bioRxiv - Cancer Biology 2024Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then seeded (1 × 104 cell/cm2) on rat tail collagen type 1-coated permeable Transwell membranes (12 mm; no. 38023, StemCell Technologies) until confluent ...
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bioRxiv - Immunology 2022Quote: ... 07180)-coated tissue culture plates and split in a ratio of 1:6 to 1:12 every four to six days with Gentle Cell Dissociation Reagent (STEMCELL Technologies, 07174). Alveolar differentiation was induced as previously described (Riva et al. ...
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bioRxiv - Neuroscience 2022Quote: ... five to seven day old iPSCs cultures were then used to generate cluster of differentiating 43 positive (CD43+) hematopoietic progenitor cells (HPCs) following a 12 day commercially available kit (STEMdiff Hematopoietic Kit; Stemcell Technologies # 05310). IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923 ...
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bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
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bioRxiv - Microbiology 2024Quote: ... mTSR+ media was replaced with fresh media A and the protocol was followed for the next 12-14 days as per kit instructions (Stem Cell Technologies). On day 12 and day 14 ...
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bioRxiv - Genomics 2024Quote: ... we seeded thawed iPSC at passage 12 (P12) or above in Matrigel-coated 6-well plates in mTESR1 media (STEMCELL Technologies (SCT), Vancouver ...
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bioRxiv - Genomics 2024Quote: ... 4.5–9 x 104 cells were plated onto 12-well plates pre-coated with 80 µg/µl growth factor- reduced Matrigel in mTeSR1 (StemCell Technologies; 85850) and 10 µM Y-27632 ...
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bioRxiv - Cancer Biology 2024Quote: Primary cells were seeded in 12-well plate at a density of 1×106 cells per well in 2 ml StemSpan™SFEM II media (STEMCELL Technologies) with growth factors cocktail (100 ng/ml SCF ...
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bioRxiv - Neuroscience 2020Quote: ... cells at week 3 of terminal differentiation were switched to BrainPhys medium (STEMCELL Technologies), and experiments were conducted after a total of 5–6 weeks of differentiation ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented for 3 days with 10 μM Rock inhibitor Y-27632 (StemCell Technologies, 78003).
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bioRxiv - Genetics 2021Quote: ... iPSC cultures were passaged every 3-4 days with Accutase (Stem Cell Technologies, 07920) or ReLeSR (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in the presence of 3 μM CHIR-99021 (Stem Cell Technologies). Then cells were cultured for 1 day without cytokines ...
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bioRxiv - Immunology 2024Quote: ... 3 ml of prewarmed collagenase type IV (1mg/mL) digestion medium (StemCell Tech 07909) was added to cell pellet ...
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bioRxiv - Developmental Biology 2021Quote: ... Scleral punches were initially placed into wells of a 96 well culture plate with 50 μl of N2 medium [Ham’s F-12/DMEM containing 1x N2 supplement (Stem Cell Technologies, Vancouver, BC) until all sclera were obtained ...
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bioRxiv - Cancer Biology 2020Quote: GFP positive neural tube regions were dissected after a DispaseI-DMEM/F-12 treatment (Stem cell technologies 1U/ml #07923; 37°C, 30min). Single cell suspensions were obtained by 3 minutes incubation in Trypsin-EDTA 0.05% (Life technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... and 105 cell/well were plated as small colonies (10-20 cell/colony) on Vitronectin-coated 12-well plates using mTeSR1 media (STEMCELL Technologies, Catalog# 12604013) supplemented with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was centrifuged again at 200 g for 12 minutes and then dissociated with a 200μl pipette and plated in Neurocult proliferation medium (Stem cell Technology, BC, CA) supplemented with EGF (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... BMDC were resuspended in PBS in a 12 × 75 mm polypropylene tube and placed in a EasySep™ magnet (Stemcell Technologies, Vancouver, CAN) for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at a density of 1.1×105 cells/well onto a 12 mm Transwell (ref 38023, STEMCELL TECHNOLOGIES, Saint Égrève, France) and grown in KSFM until cell confluency ...