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Citations for Stemcell Technologies :
151 - 200 of 1183 citations for 3 3 2 3 Dimethyl indol 1 yl 2 hydroxy propylamino propan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... culture medium is switched to E6 medium containing 3 µM CHIR99021 (STEMCELL Technologies, cat # 100-1042), 25 ng mL-1 BMP4 and 50 ng mL-1 FGF2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were minced into ∼1–2 mm pieces before digesting with 1 × Collagenase/Hyaluronidase (STEMCELL Technologies, Catalog #: 07912) and 100 μg/mL DNase I (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human recombinant Fibroblast Growth Factor 2 (FGF2, STEMCELL Technologies, 78003.1), 20 ng/mL animal component-free human recombinant Glial Cell Derived Neurotrophic Factor (GDNF ...
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bioRxiv - Immunology 2023Quote: ... Isolated cells were cultured for 1-2 weeks in StemSpan Medium (Stemcell Technologies) supplemented with 10 ng/ml interleukin (IL)-3 ...
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bioRxiv - Genomics 2024Quote: ... then cells were replated 1:2 in STEMdiff Neural Progenitor Medium (Stemcell Technologies) for NPC expansion.
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bioRxiv - Genomics 2024Quote: ... HPCs were plated at 22,000 cells/cm2 onto a Matrigel-coated 6-well plate containing 2ml of Microglia differentiation medium (Basal Medium with supplement 1 and 2 at 1:9 and 1:225, respectively; STEMCELL Technologies, #100-0019). Cells were supplemented with fresh half-medium every other day from day 0 to day 10 ...
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bioRxiv - Cell Biology 2020Quote: ... then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies) to obtain a final concentration of 104 cells ...
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bioRxiv - Cell Biology 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... and supplemented with 20 ng/ml BDNF and 20 ng/ml Neurotrophin-3 (NT3, #78074; STEMCELL Technologies) until day 43 ...
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bioRxiv - Neuroscience 2021Quote: ... approximately 3 × 106 single cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8 flex medium supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred at 3 x 106 cells to a well of an AgrreWell800 plate (Stem Cell Technologies) in STEMdiff Neural Induction Medium + SMADi (Stem Cell Technologies ...
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Single cell determination of cardiac microtissue structure and function using light sheet microscopybioRxiv - Bioengineering 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Microbiology 2020Quote: CD34+ HPCs from 3 independent donors were thawed and plated in StemSpan SFEM II media (Stemcell Technologies) containing 10% BIT serum substitute ...
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bioRxiv - Immunology 2022Quote: ... approximately 3×104 total Ai14 BM cells were plated per well in MethoCult (M3434, Stem Cell Technologies) enriched with rmSCF ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs are cultured and passaged for 3 times in STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) for up to 3 weeks ...
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bioRxiv - Immunology 2024Quote: CD34+ cells (3 × 102 cells/mL) were seeded in methylcellulose media (MethoCult™ GF+ H4435, STEMCELL Technologies) and incubated at 37°C with 5% CO₂ for 16 days ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 1% N2 supplement and 2% NeuroCult S1 supplement (STEMCELL Technologies, Vancouver, BC). After one day media was removed and replaced with a thin layer of phosphate buffered saline ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 ng/mL FGF-2 (RnD Systems) and 1 µM CHIR99021 (Stem Cell Technologies) with daily medium exchange for 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 hiPSCs were cultured in mTeSR™1 medium (Stem Cell Technologies) on Matrigel (Corning)-coated plates following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... and routinely split 1-2 times a week with ReLesSR™ (Stem Cell Technologies) without ROCKi as described previously 43 ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 min followed by a 3 min treatment with 5 mg/mL dispase (Stem Cell Technologies, 07913) and 1 mg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies), CTS Immune Cell serum replacement (Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Neuroscience 2024Quote: ... Approximately 3 × 10^6 cells were placed in each well of an AggreWell 800 plate (STEMCELL Technologies # 34811) containing Stemflex medium enriched with 1X Revita supplement ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of 2% rat serum (Stemcell technologies) was added to each well for 15 minutes at room temperature and proceeded to further staining without a washing step.
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow aspirates were diluted 1:1 with PBS (+ 2% FBS) and layered on a Lymphoprep gradient using SepMate tubes (STEMCELL Technologies). After centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×103 cells were resuspended in 1 ml of complete MammoCultTM Basal Medium (Stemcell Tech) and seeded in ultralow attachment plates ...
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bioRxiv - Immunology 2020Quote: ... FcγRII or FcγRIII block was performed by addition of receptor specific mAbs (Stem cell technologies, IV.3 and 3G8) at a 1:100 dilution at the beginning of the incubation period ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... For stem cell maintenance N2B27 medium was supplemented with 3 μM CHIR99021 (Chir) (Catalog no. 72054, Stem Cell Technologies), 1 μM PD0305901 (Catalog no ...
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bioRxiv - Immunology 2024Quote: ... Then they were added to 3 ml of prewarmed (37°C) dispase [1U/mL] digestion medium (StemCell Tech 07923). The samples were incubated at 37°C for 12 min while inverting the tube every 3 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 U/ml EPO) and the indicated CM-272 concentrations into six-well plates (SmartDish™ 27370, Stemcell technologies). Cells were cultured for 8-10 days at 37 °C and 5% CO2 and colonies (defined as a collection of at least 10 cells ...
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bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole blood was diluted 1:1 with phosphate-buffered saline (PBS) containing 2% FBS and then layered onto Lymphoprep (STEMCELL Technologies, 07851) in SepMate-50 isolation tubes (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... Purified cell populations were expanded in endothelium maintenance media consisting of conditioned media diluted at 1:1 ratio with StemPro-34 SFM supplemented with 2 µg/mL heparin (STEMCELL Technologies, 07980). Media was replaced every other day or until the conditioned media was depleted ...
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bioRxiv - Developmental Biology 2024Quote: ... it was switched to PXGL medium (N2B27 medium supplemented with 1 μM PD0325901, 2 μM XAV939 [STEMCELL Technologies, 72674], 2 μM Gö6983 [STEMCELL Technologies, 72462], and 10 ng/mL LIF), refreshed daily for an additional 8-10 days before passaging ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stemgent or Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured for 1 day in the presence of 100 units of IL-2 (StemCell Technologies) followed by 2 days in T cell medium alone ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were spun down (120 × g) for 3 min and resuspended in the required amount of mTeSR (Stem Cell Technologies) + Rock Inhibitor (RI) ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... at a density of ±8.75 × 104 cells/cm2 or 3 × 105 cells/ml in mTeSR medium (Stem Cell Technologies, 85850) supplemented with 1:100 RevitaCell Supplement (Gibco ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2024Quote: ... the medium was replaced by Stemfit AK02N (A + B) supplemented with CHIR99021 (3 μM, #72052; STEMCELL Technologies, Vancouver, BC, Canada) and ActivinA (10 ng/mL ...