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Citations for Stemcell Technologies :
151 - 200 of 1167 citations for 1 3 Methoxyphenyl 6 7 dimethyl 6 azoniabicyclo 3.2.1 octane bromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Supernatants were removed and cells were resuspended in Noci-2 medium + CEPT and plated at 5.5 x 106 cells/well into 6-well AggreWell 800 plates according to manufacturer’s instruction (STEMCELL Technologies) for free-floating suspension culture and nocisphere formation ...
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bioRxiv - Cell Biology 2020Quote: ... hiPSC colonies were collected from a 6 well MEF-coated plate using Gentle Cell Dissociation Reagent (Stemcell Technologies, CA) and seeded into Matrigel (BD ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated into single cells and clustered in AggreWell 400 6-well plates (Cat. No. 34425, STEMCELL Technologies) with DMEM + 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... and plated into a poly-hema treated 6-cm Petri dish in media containing mTeSR1 (StemCell Technologies, Cambridge, MA) and 10μM rock inhibitor (RI ...
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Immunology 2022Quote: ... Approximately 45mg of each frozen liver sample was placed in a 6-well tissue culture plate (Stem Cell Technologies) with 1 ml TST (2 mL of 2X ST buffer + 120 µL of 1% Tween-20 + 20 µL of 2% BSA brought up to 4 ml with nuclease-free water) ...
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bioRxiv - Cell Biology 2024Quote: ... The hiPSCs were maintained on Corning Matrigel-coated 6-well tissue culture plates with mTeSR-Plus medium (STEMCELL Technologies). Cells were passaged every 4 days using ReLeSR media (StemCell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended vesicles were layered onto a 6-18% Optiprep™ 11-step discontinuous velocity gradient (Stemcell Technologies, Vancouver, Canada), and centrifuged for 90 min at 176,000 × g in a SW40Ti rotor as previously described (48 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on 5% laminin at 50,000 cells per well of 6 well-plate and incubated in full growth medium: PneumaCult-Ex medium (Stemcell Tech), glutamax supplement (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 2mL per well T0 media and deposited into AggreWell 400 6-Well plates (StemCell Technologies 34425) prepared with Aggrewell Rinsing Solution (StemCell Technologies 07010 ...
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bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Neuroscience 2022Quote: iPSCs were cultured in feeder-free conditions on Matrigel-coated (0.08 mg/well) 6-well tissue culture plates in mTesR1 media (StemCell Technologies) at 37 °C and 5 % CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were plated on 6-well plates coated with geltrex (Life Technology) and cultured in TeSR-E8 medium (Stemcell Technology). For hiPSC-CMs differentiation ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
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bioRxiv - Neuroscience 2023Quote: ... was pipetted into the cuvette and incubated for 10 minutes at 37°C and then transferred to a fresh well of a Matrigel coated 6-well plate with 2ml of mTeSR1 supplemented with ROCK-inhibitor (10μM, StemCell Technologies). Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... APCs were seeded onto Matrigel coated 6 well plates at 200,000 cells per well with STEMdiffTM Astrocyte Maturation medium (STEMCELL Technologies). After 2 weeks ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were seeded on pre-treated (0.167 mg/mL Matrigel) 6-well plates and cultured in TeSR-E8 iPSC culture medium (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... seeded in PLO/Lm coated 6-well plates (EBs produced in one well of AggreWell™ 400 24-well plate were usually seeded into one well of a 6-well plate) and maintained in AAN2 medium (0.5% N2 Supplement (ref.07152, Stemcell Technologies), 200 mM AA-ascorbic acid (ref.12084947 ...
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bioRxiv - Bioengineering 2022Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Then at day 10 ...
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
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bioRxiv - Immunology 2022Quote: ... diluted in MethoCult GF-M3434 medium and plated at 20,000 cells/35 mm well in a 6-well plate of low-adherence culture-ware (STEMCELL Technologies, Inc.), in triplicate ...
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Single cells were plated (2 x 106/well) in Matrigel-coated 6-well plate in mTeSR1 plus growth medium (STEMCELL Technologies), supplemented with 10 mM Y-27632 ROCK inhibitor (Calbiochem ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Neuroscience 2022Quote: ... the dissociated cells were transferred to individual low-adherence V-bottom 96-well plates (S-Bio Prime) in 150μl TeSR5/6 media (StemCell Technologies®) with 50μM Rock inhibitor Y-27632 (Tocris BioScience) ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in new 6-well plate using mTeSR™ plus medium supplemented with 10 μM Y-27,632 Rock inhibitor (StemCell Technologies). Pluripotency of all lines was established by analyzing the expressions of canonical stemness genes (SOX2 ...
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleofected cells were transferred to a new matrigel-coated well in a 6-well plate in 4mL of E8 medium containing 10μM ROCK inhibitor Y-27632 (StemCell Technologies #72307). Medium was replaced the following day with fresh E8 ...
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
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bioRxiv - Bioengineering 2023Quote: ... gift from the Seidman Lab) or GFP-DSP reporter (WTC; Allen Institute AICS-0017 cl.6) were cultured in mTeSR1 media (StemCell Technologies) on Matrigel (Corning ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-positive cells were plated into a matrigel-coated 6 cm petri dish in mTeSR supplemented with 1x CloneR (Stemcell Technologies). Individual colonies were manually picked into 96 wells ∼10 days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... pan CD3+ murine T cells were isolated from single cell splenocyte suspensions of 6 – 12-week-old mice through a negative selection process (STEMCELL Technologies). T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm ...
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: ... We initiated the organoid development by plating hiPSCs at density 20- 30% in TeSR1 medium on a 6-well plate coated with human embryonic stem cell (hESC)-qualified basement membrane matrix (STEMCELL Technologies). The next day ...
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bioRxiv - Cell Biology 2024Quote: ... the media was changed to remove the ROCK inhibitor and was maintained for 6 hours in in mTeSR plus (Cat# 100-0276, StemCell Technologies). The media was then replaced with DMEM/F12 (Cat# 11320033 ...
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bioRxiv - Bioengineering 2024Quote: Our protocol began with cultivating human pluripotent stem cells (H9) in a Matrigel-coated 6-well plate using a stem cell feeder-free culture medium (mTeSR1, STEMCELL Technologies). For routine passaging ...
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bioRxiv - Cell Biology 2024Quote: ... The microglial differentiation was performed on thawed HPCs (200,000 cells per well of a 6-well plate) using the STEMdiffTM Microglia Culture System (cat. 100-0019 and 100-0020, STEMCELL Technologies) for 24 days according to manufacturer’s directions ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were passaged in small aggregates (1:7/1:10 depending on the line) using ReLeSR (StemCell Technologies). 10 μM Y-27632 (ROCK inhibitor ...