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Citations for Stemcell Technologies :
1901 - 1950 of 2099 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from spleens of P14 donor mice by negative selection using the EasySepTM Mouse CD8+ T-cell isolation kit (STEMCELL Technologies). P14 cells were activated in vitro for 24-28 hours with 100U/mL recombinant IL-2 ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, #TIB-9) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, #03800). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Bioengineering 2023Quote: ... splenic T lymphocytes were isolated from Cas9 transgenic mice using negative selection with the EasySep Mouse T Cell Isolation Kit (Stemcell Technologies) and then activated with Dynabeads Mouse T-Activator CD3/CD28 beads (Cat # 11456D ...
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bioRxiv - Immunology 2023Quote: ... Naive B cells were isolated by negative magnetic activated cell sorting using the EasySepTM Mouse B cell Isolation Kit (StemCell Technologies). 30 million B cells were injected i.p ...
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bioRxiv - Microbiology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, France) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, Canada). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Immunology 2023Quote: ... ETRAMP-specific CD4+ T cells were enriched using APC- and PE-conjugated tetramers and EasySep APC and PE Positive Selection Kits (STEMCELL Technologies). Cells were then stained with fluorescent dye-conjugated antibodies (see Table I ...
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bioRxiv - Cancer Biology 2023Quote: CD8 T cells were isolated from the lymph node of naïve mice with unactivated Confetti cassette using EasySep™ Mouse CD8+ T Cell Isolation Kit (StemCell Technologies) and macrophages were sorted from the tumors via FACS sorting ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from peripheral lymph nodes (inguinal, axillary, brachial and superficial cervical) by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies). CD4+ T cells were stimulated in 96 well flat-bottomed plates coated with 20mg/ml αCD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
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bioRxiv - Immunology 2022Quote: ... NK cells were either isolated from cryopreserved PBMCs by either negative selection using the EasySep NK cell enrichment kit (StemCell technologies) or by depletion of T cells with human CD3 MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells from spleens were negatively enriched using an EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853) according to the manufacturer’s instructions (STEMCELL Technologies). Enriched CD8+ T cells (92% - 96% purity ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Cancer Biology 2022Quote: ... apoptotic and dead cells were removed using immunomagnetic cell separation with the Annexin Dead Cell Removal Kit (StemCell Technologies, Cat# 17899) and EasySepTM Magnet (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated from spleens and lymph nodes of P14 Tcf7GFP or OTI Tcf7GFP mice using the EasySepTM Mouse CD8+ T cell Isolation Kit (Stemcell Technologies), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Immunology 2022Quote: ... or GZB-cre Zbtb20-fl/fl CD45.1 OT-I mice (KO) and naive CD8 T cells purified using EasySep Mouse Naive CD8 T Cell Isolation Kits (catalog no. 19858A; STEMCELL Technologies). Fifty thousand naive OT-I cells were retro-orbitally injected into congenic B6 recipient mice ...
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bioRxiv - Microbiology 2022Quote: ... Loosely adherent immature BMDCs were collected and then CD11c+ DCs were enriched using CD11c positive selection kit (Stemcell Technologies, Cambridge, MA). Twenty-four hours after culture of purified subsets ...
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bioRxiv - Immunology 2022Quote: ... and B and T cells were isolated using EasySep Negative selection Mouse B and T cell Enrichment Kits according to the manufacturers protocol (StemCell Technologies). Cells were then suspended in transfer buffer (10 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse T-cells were isolated from mouse PP/lymph nodes/spleens and tumors using EasySep Mouse CD8+ T Cell Isolation kit (StemCell Technologies). For FACS analysis ...
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bioRxiv - Genetics 2023Quote: ... Common karyotypic abnormalities were measured using the qPCR-based hPSC Genetic Analysis Kit following manufacturer’s instructions (STEMCELL Technologies; cat. no. 07550). Cell lines with true karyotypic abnormalities were not used in this study.
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bioRxiv - Immunology 2024Quote: ... OT-I+/- or total CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection using the EasySepTM Mouse CD8+ T cell isolation kit (#19753, StemCell Technologies). For in vitro experiments ...
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bioRxiv - Immunology 2024Quote: ... The resulting hybridoma cells were selected on semi-solid methylcellulose and grown in 96-well plates using the ClonaCell-HY Hybridoma Cloning Kit (Stemcell Technologies). Hybridomas secreting SH-01-reactive IgG were identified by several rounds of ELISA screening ...
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bioRxiv - Neuroscience 2024Quote: ... mTESR plus medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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bioRxiv - Molecular Biology 2024Quote: ... femur and tibia were flushed and neutrophils (PMN) enrichment was performed with the EasySep mouse neutrophil enrichment kit (Stem Cell Technologies). PMN ROS production upon stimulation with PMA (100 nM ...
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bioRxiv - Neuroscience 2024Quote: hiPSC derived neural progenitor cells (NPCs) were generated using the embryoid body protocol and STEMdiff SMADi Neural Induction Kit (STEMCELL Technologies). From these NPCs ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells (OT-I cells) were isolated by negative selection with the EasySepTM Mouse CD8 T cell isolation kit (Catalog #19853, Stemcell Technologies) following the manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: ... Cells were filtered through a 70 µm nylon strainer and further purified with the EasySepTM mouse neutrophil enrichment kit (StemCell Technologies), following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... EB-HSPCs, and primary mPBSCs from a healthy donor (female, 30 years) were differentiated using the StemDiff T Cell Kit (STEMCELL Technologies) with minor modifications to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T were isolated from the spleen and LN of OT-II TCR Tg GFP+ mice using the Easysep Mouse CD4+ T cell isolation kit (Stemcell Technologies) or MojoSort Mouse CD4 Naïve T cell isolation kit (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... with minor modifications.18 CD34+ cells were isolated directly from PBMCs or from enriched HSPCs (RosetteSep Hematopoietic Progenitor Enrichment Cocktail Kit, Stemcell Technologies) via immunomagnetic isolation (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: The ALDH activity of oral cancer cells co-cultured with CAF was measured using the ALDEFLUOR assay kit (Cat. #01700, StemCell Technology), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were plated at density of 65,000 cells per well in Neural Induction Media (STEMdiff SMADi Neural Induction Kit STEMCell Technologies, 08581) in a 96-well v-bottom plate to form neural aggregates ...
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bioRxiv - Cancer Biology 2024Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then harvested and stained using the Annexin V Apoptosis Detection Kit with 7-AAD (STEMCELL Technologies, Vancouver, BC, Canada), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the mouse spleen and injected with mEERL cells with scramble or Marchf8 sgRNA using the CD8+ T-cell Isolation Kit (STEMCELL Technologies). The cells were labeled with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: Splenic B cells from BACtg and wild type littermate control mice were isolated using the EasySep Mouse B Cell Isolation Kit (StemCell Technologies) and rested for 30 minutes and then either left unstimulated or stimulated with the addition of 10 μg/ml goat anti-IgM F(ab’)2 crosslinking antibody (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2024Quote: RNA extraction and quantitative PCR: Tumor infiltrating myeloid cells were isolated from tumors either using a CD11b isolation kit (catalog # 18970, StemCell Technologies) or by cell sorting ...
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bioRxiv - Cancer Biology 2024Quote: CD8+ T cells were isolated from the spleens of C57BL/6J or mice with magnetic bead-based CD8 T cell isolation kit (Cat # 19853, StemCell Technologies). T cells from naïve mice were stained with 5µM Cell Trace Violet (Cat# C34557 ...
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bioRxiv - Bioengineering 2024Quote: ... CD8+/CD4+T cells (for OT-I/OT-II mice) were isolated from this suspension using the EasySep Mouse CD8+/CD4+ T Cell Isolation kit (STEMCELL Technologies; Cat#19852 for CD4+T ...
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bioRxiv - Cancer Biology 2024Quote: Mouse spleens were mechanically disrupted over a 100-µm filter and subjected to magnetic bead negative enrichment for CD8+ T cells using a CD8+ T-cell Isolation Kit (STEMCELL Technologies). Isolated CD8+ T cells were cultured in RPMI (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Neurospheres were dissociated into single cells and passaged every seven days using a chemical dissociation kit (Catalog# 05707, STEMCELL technologies, U.S.A.). Fresh neurobasal medium (containing all the supplements ...
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bioRxiv - Cell Biology 2024Quote: The differentiation of iPSCs into the three germ layers was performed using the STEMdiff™ Trilineage Differentiation Kit (STEMCELL Technologies, #05230).
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bioRxiv - Bioengineering 2024Quote: ... and endoderm was performed on 48-well plates (500 µl/well feed volume) using the STEMdiff Trilineage Differentiation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: H1 parental and modified hES cells were differentiated into PP cells using the STEMdiff™ Pancreatic Progenitor Kit (STEMCELL Technologies, 05120) according to the manufacturer’s instructions or using an adaptation of published procedures 8,10,51 with changes as described (Table S5) ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Murine T-cells were isolated from spleens of healthy 6-8-week old C57BL/6 mice using EasySep™ Mouse CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...