Labshake search
Citations for Stemcell Technologies :
1901 - 1950 of 4551 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 50 ng/mL HGF (Stem Cell Technologies catalog #78019), and 20 ng/mL Noggin (R&D Systems catalog #6057-NG ...
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bioRxiv - Bioengineering 2024Quote: ... 50 ng/mL HGF (Stem Cell Technologies catalog #78019), and 20 ng/mL Noggin (R&D Systems catalog #6057-NG ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 2 μg/mL heparin (Stem Cell Technology), 10 ng/mL FGF (Stem Cell Technology) ...
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bioRxiv - Microbiology 2024Quote: ... containing 3 μM CHIR99021 (Stem Cell Technologies, Cat.#72054), 500 μg/mL bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... lineage (Lin)-positive cells depletion was performed (StemCell Technologies) and Lin-cells were viably frozen ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested using ReLeSR dissociation reagent (STEMCELL Technologies) and passaged for a minimum of three times before commencing differentiation into cardiomyocytes.
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bioRxiv - Cell Biology 2024Quote: ... stem cell factor (SCF; 250 ng/mL; STEMCELL Technologies), IL-11 (20ng/mL ...
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bioRxiv - Cell Biology 2024Quote: ... stem cell factor (SCF; 250 ng/mL; STEMCELL Technologies), IL-3 (10 ng/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were grown in MethoCult™ M3234 (STEMCELL Technologies) with 10 ng/mL mouse stem cell factor (SCF ...
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bioRxiv - Genomics 2024Quote: ... and 0.48 µg/mL Hydrocortisone (Stem Cell Technologies, 74144) on a stir plate at 37□C and 180 rpm ...
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bioRxiv - Genomics 2024Quote: ... cells were maintained in mTeSR 1 (STEMCELL Technologies, 85850) and the medium was changed daily ...
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bioRxiv - Molecular Biology 2024Quote: ... on Vitronectin XF™ (Stem Cell Technologies, Cat. #07180) coated tissue culture plates ...
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bioRxiv - Immunology 2019Quote: Isolated HSPCs from Apoe−/−Il27ra+/− and Apoe−/−Il27ra−/− mice infused with Ang II were sorted and plated at 1×103 cells in 1ml of MethoCult® GF M3434 (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and Mononuclear cells were incubated with 1 ml of erythrocytes and the RossetteSep™ CD8+T cell enrichment cocktail to obtain Total CD8+T cells (15063; StemCell Technologies, Canada). The memory cells were eliminated using magnetic beads (8803 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Cell Biology 2021Quote: ... YTS cells were cultured in RPMI media supplemented with 10% fetal bovine serum and NK92 cells were cultured in Myelocult (Stemcell Technologies, catalogue H5100) supplemented with 200 U/ml of IL-2 (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Neuronal differentiation was performed using the protocol as described [59] with a modification that the cells were frozen at the neural progenitor stage at day 8 in CryoStor CS10 (Stem Cell Technologies #07931). Cells were thawed into LIA medium (ADF supplemented with 2mM Glutamax ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Cancer Biology 2020Quote: Clonogenic assay was realized with cells seeded into 35mm petri dish in semi-solid methylcellulose medium (Methocult™ M3231 for murin cells or Methocult™ H4230 for human cells, Stemcell Technologies). Cells were treated with the drugs for 72 hrs and centrifugated ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were needle triturated and resuspended in Mouse NSC Proliferation Media (1x Anti-Anti, 10ng/mL bFGF (#78003, Stem Cell Tech, Vancouver), 20ng/mL mEGF (#78016 ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells were extracted from blood drawn from healthy donors using density gradient separation (Lymphoprep, Stem Cell Technologies, Vancouver, BC, Canada) and cultured at 1 × 106 cells/ml in RPMI complete medium containing 1μg/ml Concanavalin A (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Erythroblasts were cultured in a serum-free mononuclear cell (MNC) medium containing the following cytokines diluted in Stem Span Serum Free Expansion Medium (Stem Cell Technologies, CA): 40□ng/mL insulin-like growth factor 1 (IGF1) ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from spleens of WT C57BL/6 mice by negative selection using the EasySep™ Mouse CD8+ T cell isolation kit (Stem Cell Technologies) according to the manufacturer’s protocol and cultured in complete media ...
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bioRxiv - Immunology 2019Quote: ... naïve and memory CD4+ T cells were isolated from PBMCs using EasySep® naïve CD4+ T cell isolation kit and memory CD4+ T cell enrichment kit (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Transduced cells were expanded to 10 cm plates and 10 vials (2M cells/vial) of each cell line was cryopreserved in CryoStor® (StemCell Technologies, 07930) for further use.
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bioRxiv - Cancer Biology 2020Quote: ... Cryopreserved MDS cells were thawed and cultured in StemSpan™ + cytokines (10ng/ml hIL3, 10ng/ml hSCF, 5ng/ml hIL6) overnight before plating cells in Methocult H4034 Optimum (Stem Cell Technologies) at a density of 1-2 × 104 cells per mL with DMSO ...
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bioRxiv - Immunology 2020Quote: ... Primary T cells were isolated from peripheral blood of healthy donors by negative selection using RosetteSept™ Human T Cell Enrichment Cocktail (STEMCELL Technologies, 15061) and activated with Dynabeads® Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were isolated from the PBMC fraction using EasySep® naïve CD4+ T cell isolation kits and memory CD4+ T cell enrichment kits (StemCell Technologies, Meylan, France) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The cell mixture underwent centrifugation at 200g for 10min and then cultured using NeuroCult proliferation medium (Cat#05702, Stem Cell Technologies, Vancouver, Canada). The cells were maintained at standard conditions in a humidified 37 C° incubator containing 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Immunology 2022Quote: ... CD8+ and CD3+ T cells were isolated from PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit and EasySep™ Human T Cell Isolation Kit (both from STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2022Quote: ... Splenic T cells were enriched using a magnetic-based commercial CD8 T cell negative selection kit (StemCell Technologies, BC, Canada; Supplemental Figure 3A) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... media was replaced every other day with neuronal differentiation media prepared based on the Stem Cell Technology protocol: 10 ml of the BrainPhys™ Neuronal Medium (#05790; Stem Cell Technology) supplemented with 200 µl of NeuroCultTM SM1 Neuronal Supplement (#05711 ...
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bioRxiv - Cell Biology 2023Quote: ... assay done by culturing 100-sorted cells LIN-CD34+ CD38- CD9+ or CD9- in to MethoCult™ H4434 with human cytokine (Stem Cell Technologies) and colony formation score at day 12-14 of incubation in 37°C.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were subcultured onto Matrigel at the first passage to avoid loss of the differentiated cells before tolerating the switch to MesenCult-ACF attachment substrate (Stem Cell Technologies, 05448). For passage two or above ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at a density of 1.1×105 cells/well onto a 12 mm Transwell (ref 38023, STEMCELL TECHNOLOGIES, Saint Égrève, France) and grown in KSFM until cell confluency ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...