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Citations for Stemcell Technologies :
1601 - 1650 of 1886 citations for Rat Vasoactive Intestinal Polypeptide Receptor 1 VIPR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Assays were performed on purified human classical monocytes isolated using immunomagnetic negative sorting (EasySep Direct Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, MA). As we have previously described 88 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mice were anesthetized with a solution consisting of 200 mg/kg ketamine and 20 mg/kg xylazine and perfused with PBS before neoplastic tissue was processed with the NeuroCult™ enzymatic dissociation Kit (StemCell Technologies, 05715) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... by plating them at 125.000 cells/cm2 on Matrigel and growing the cells in STEMdiff™ Neuron Differentiation Kit (Stem Cell Technologies, #08500) medium for one week with daily medium changes.
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bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Immunology 2022Quote: ... Splenic T cells were enriched using a magnetic-based commercial CD8 T cell negative selection kit (StemCell Technologies, BC, Canada; Supplemental Figure 3A) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Tcon and Treg cells were isolated from the spleen by pre-enrichment with EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, Cat# 19852), and FACS sorting ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Cell Biology 2023Quote: ... The middle layer containing PBMCs were then enriched for primary human NK cells (hNK) by negative selection using the EasySep Human NK Cell Enrichment Kit (StemCell Technologies, Cat# 19055), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were filtered through a 70 μm nylon strainer and further purified with the EasySep mouse neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... After 24 hours cells were washed with D-PBS before media was exchanged for astrocyte differentiation medium (STEMdifftm Astrocyte differentiation kit #100-0013, StemCell Technologies, Cambridge, UK) and a full media exchange was completed every day for 7 days ...
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bioRxiv - Developmental Biology 2023Quote: PP cells generated using the STEMdiff™ Pancreatic Progenitor Kit and expanded under CINI conditions were dissociated into single cells using AccutaseTM (STEMCELL Technologies, 07920) at 37 °C and then resuspended in PEP medium (Table S4 ...
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bioRxiv - Neuroscience 2023Quote: ... A crude live cell mixture was obtained by filtration and negative selection using Easy Eights EasySep Magnet and Dead Cell Removal kit (Stemcell Technologies, Catalog # 17899). Cells were further en-riched with positive CD90.2 selection (Stemcell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 24-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276) and 1x Penn/Strep from a 100x stock (Mediatech ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Bioengineering 2024Quote: The primary T cells were obtained from peripheral venous blood of a healthy human donor using the EasySep™ Direct Human T cell Isolation Kit (STEMCELL TECHNOLOGIES, #19661) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from single-cell suspensions from spleen tissue by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (# 19852, StemCell Technologies, Cambridge, MA) and differentiated into different T helper (Th ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: Total CD4+ T-cells were obtained by negative selection from the spleen of wild-type mice using EasySepTM magnetic separation per kit instructions (product number 19851, STEMCELL Technologies, Vancouver, Canada). Purified total CD4+ were enumerated and 5×105 total CD4+ T-cells were injected via tail vein to recipient mice ...
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bioRxiv - Bioengineering 2023Quote: ... CD31+ EPCs were purified using a FITC-conjugated human CD31 antibody (Miltenyi, cat# 130-117-390, clone AC128) and the EasySep Human FITC Positive Selection Kit II (Stemcell Technologies, cat# 18558). Purified EPCs were seeded into collagen IV (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... were cultured with lymphocytes (2.5 × 104) magnetically sorted frommousespleen singlecell preparations using MACs columns and the EasySep™ Mouse TCell Isolation Kit (StemCell Technologies Cat# 19851) for a total of 6d with 20 units/mL of IL-2 ...
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bioRxiv - Neuroscience 2021Quote: ... the iPSC’s were transitioned from Essential 8™ medium to mTeSR™1 medium (StemCell Technologies) for further expansion and analysis ...
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bioRxiv - Cell Biology 2020Quote: ... BM cells (105) were aliquot in 1 mL of Iscove’s modified Dulbecco’s medium (StemCell Technologies #07700) then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated with Gentle Cell Dissociation Reagent (STEMCELL Technologies 07174 and passaged 1:4 (C1) or 1:6 (C2 ...
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bioRxiv - Neuroscience 2021Quote: ... On day -1 cells were plated into mTeSR1 medium with 0.5 μM Thiazovivin (STEMCELL technologies # 72252) onto hESC-matrigel coated (Corning # 354277 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were adjusted to 1×105 cells/ml final concentration in H4230 methylcellulose medium (STEMCELL Technologies) supplemented with 10% 5637-CM as a stimulant and then plated in 35-mm petri dishes in duplicate and allowed to grow for 7 days in a humidified CO2 incubator (5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... ES cells were maintained as feeder-free cells in mTeSR™1 medium (Stem Cell Technologies). Cells were allowed to spread overnight prior to transduction ...
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bioRxiv - Developmental Biology 2021Quote: ... hESC colonies were fragmented and split in a 1:20 ratio following ReLeSR treatment (StemCell Technologies). For directed differentiation experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... Encapsulated aggregates were allowed to recover for 24 hours in mTeSR™-1 medium (STEMCELL Technologies) in rotary suspension ...
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bioRxiv - Cell Biology 2020Quote: ... Jurkat cells (Clone E6-1, ATCC® TIB-152(tm)) and PBMCs (Stemcell technologies, Catalog # 70025.1) were cultured at 0.5–1.5 × 10^6 cells/ml in RPMI 1640 media (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... CD34+ cells (1 × 105/mL) were suspended in serum-free expansion medium II (Stem Cell Technologies) with 50 ng/mL stem cell factor (PeproTech ...
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bioRxiv - Physiology 2021Quote: ... cell pellets were resuspended in pre-warmed HBSS containing 1 U/ml Dispase (07913, Stemcell Technologies) and 10 µg/ml DNase I (11284932001 ...
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bioRxiv - Immunology 2021Quote: ... 1000 sorted cells were plated in 1-2ml MethoCultTM GF M3434 (Stemcell Technologies, Catalogue no.-03434) with 50-100 ng/ml M-CSF (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology, # 10981) and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology ...
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bioRxiv - Developmental Biology 2022Quote: H9 hESCs were cultured in mTESR-1 medium supplemented with 10mM ROCKi (Y27632; StemCell Technology 72304) for 24hrs ...
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bioRxiv - Immunology 2022Quote: ... Biopsies were collected during routine endoscopy and frozen in 1 ml of CryoStor CS10 (StemCell Technologies) at −80 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng/ml FGF-2 (RnD Systems) and 1 μM CHIR99021 (Stemgent or Stem Cell Technologies). Mesoderm induction required daily medium exchanges for 3 days ...
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bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
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bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: OT1 CD8+ T cells from Wild type or the knockout mice mentioned above were purified from spleens using EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853, STEMCELL Technologies, Vancouver, CA). Isolated CD90.1+ OT1 CD8+ T cells were injected into CD90.2+ C57BL6 recipient mice at a concentration of 106 cells/100ul PBS through the retro-orbital route.
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bioRxiv - Cell Biology 2022Quote: We isolated CD34+ cells from human cord blood and BM samples using an EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, Canada); sample purity of >95% was confirmed using flow cytometry ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were pelleted by centrifugation at 300 g for 10 min and resuspended at 108 cells/mL in EasySep™ buffer and B lineage cells were isolated using an EasySep™ mouse B cell negative isolation kit (StemCell Technologies, #19854). For serum ...