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Citations for Stemcell Technologies :
1601 - 1650 of 1767 citations for Rat Insulin Like Growth Factor Binding Protein 6 IGFBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cells were collected after an additional two days and pelleted for dead cell removal based on Annexin V using the Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, #17899).
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bioRxiv - Microbiology 2022Quote: Memory CD4+ T cells were enriched by negative selection using the EasySep Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies #19157) following the recommended protocol ...
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bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Primary CD4+ T cells were isolated from spleens for NOD mice using Stemcell murine CD4+ T cell positive selection kit (Stemcell Technologies Inc).
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were harvested from CD1 wildtype P25 mice and processed using the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Stemcell Technologies, 19858). Following isolation ...
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bioRxiv - Immunology 2023Quote: ... T cells from the transgenic mouse splenocytes were enriched by magnetic isolation using the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies). T cells were centrifuged and resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: The enzymatic activity of RALDH2 in BMDCs and MLN cells was assesses using the ALDEFLUOR Kit (#01700, STEMCELL Technologies, Vancouver, BC, Canada). DCs were pre-gated as CD11c+/MHC IIhigh cells and RALDH2 activity was detected by a flow cytometric analysis.
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2024Quote: For further examination of neutrophil granulocytes we isolated granulocytes with the EasySep® Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Cat# 19666), following the instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... the spheres were chemically dissociated after seven days of plating, using the NeuroCult Chemical Dissociation Kit (mouse, #05707) from Stem Cell Technologies, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human blood neutrophils from healthy controls were isolated using negative sorting with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies 19666), loaded with DCFDA ...
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bioRxiv - Neuroscience 2023Quote: Human cerebral organoids were generated from WT and PCDH12-KO RUES1 hESCs and WT GM03651 and GM03652 iPSCs with the STEMDiff Cerebral Organoid Kit (StemCell Technologies, 08570) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... filtered through 40 µm cell strainers and T cells enriched by negative selection using an EasySep™ pan-T cell enrichment kit (Stemcell Technologies) as per manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2023Quote: ... DE and LPM precursors were induced once seeded hiPSC-formed colonies by the Activin induction using the STEMdiff Definitive Endoderm Kit (StemCell Technologies, 05110) for 72 hours ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells using a combined strategy of incorporating primitive hematopoiesis via a WNT switch(77)and sorting and replating CD235a+ cells in Medium B from a commercially available kit (StemCell Technologies #05310). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ progenitor cells were enriched using magnetic CD34 microbead kit (Miltenyl Biotec, San Jose, California) and expanded overnight in SFEMII (Stem Cell Technologies) supplemented with SCF (100 ng/mL) ...
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bioRxiv - Genomics 2023Quote: ... liver samples in cohort 2 were reduced of dead cell burden using the EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, 17899) to improve cell viability to >70% ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were subsequently isolated from the PBMC population by immunomagnetic negative selective with the EasySep Human T Cell Isolation kit (StemCell Technologies, Canada). Isolated T cells with >95% viability and >95% purity were either cryopreserved or directly cultured for subsequent experiments ...
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bioRxiv - Genetics 2023Quote: Peripheral blood mononuclear cells were isolated by standard Ficoll gradient centrifugation and classical monocytes isolated using EasySep™ Human Monocyte Isolation Kit from Stemcell Technologies according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were purified from fresh BM cells using MicroBead kit (Miltenyi Biotech 130-046-702) and subsequently cryopreserved in CryoStor® CS5 (STEMCELL Technologies).
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bioRxiv - Neuroscience 2023Quote: Mature astrocytes were created from astrocyte precursor cells using the STEMdiff Astrocyte Maturation media kit from STEMCELL Technologies (Cat No. 100-0016) following their guidelines for Matrigel-coated 6-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse T cells were purified from mouse splenocytes using a negative selection EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies). Mouse splenocytes were prepared from spleens collected from 8-18 weeks old male BL6 mice purchased from Orient bio ...
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bioRxiv - Immunology 2023Quote: ... CD14+CD16-monocytes were isolated from peripheral blood mononuclear cells using immunomagnetic negative selection (EasySep Human Monocyte Isolation Kit, Stem Cell Technologies).
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bioRxiv - Microbiology 2023Quote: ... Human CD34+ cells were isolated from cord blood using EasySep™ human cord blood CD34 positive selection kit II (STEMCELL Technologies #17896), which were then cryopreserved in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2023Quote: ... Primary B cells were isolated from spleens of 2–3 month old C57Bl/6N mice by negative selection with EasySep™ Mouse B Cell Isolation Kit (# 19854, STEMCELL Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... apoptotic and dead cells were removed by immunomagnetic cell separation using the Annexin Dead Cell Removal Kit (StemCell Technologies, cat. no. 17899) and EasySep Magnet (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from pooled splenic cell suspensions by magnetic negative selection using EasySep™ Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Self-organizing cerebral organoids were derived from induced pluripotent stem cells (iPSC) using the STEMdiff™ Cerebral Organoid Kit developed by STEMCELL Technologies™ (catalogue # 08570) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from human peripheral blood using EasySepTM Human CD8+ T Cell Isolation Kit (Stemcell Technologies, Vancouver, BC, Canada) and stained with 10 μM CellTraceTM Violet Cell Proliferation Kit (VPD ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
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bioRxiv - Bioengineering 2024Quote: ... CD56+CD3- NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: ... mTSR+ media was replaced with fresh media A and the protocol was followed for the next 12-14 days as per kit instructions (Stem Cell Technologies). On day 12 and day 14 ...
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bioRxiv - Bioengineering 2024Quote: ... Neutrophils were retained in solution through negative immuno-magnetic separation through the EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Neutrophils were incubated in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b+ microglial cells were isolated by magnetic bead enrichment using the EasySep™ Mouse CD11b Positive Selection Kit (STEMCELL Technologies, Cat. 18970), according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... B cells were then enriched by negative selection using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (17963, Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neural Precursor Cells (NSCs) were generated from human induced pluripotent stem cells (iPSCs) using STEMdiff™ SMADi Neural Induction kit (STEMCELL Technologies) via dual SMAD inhibition as per the manufacturers protocol ...
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bioRxiv - Cancer Biology 2024Quote: Primary human NK cells were isolated from PBMCs from three donors by negative selection using the EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies 17955), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 100,000-200,000 CD4+ T cells per well were plated in 96-well V-bottom plates and rested for 1.5 hours at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were washed in PBS and resuspended in PBS containing 2%FBS and 2 mM EDTA before negative selection using EasySep Mouse Pan-B Cell Isolation Kit (StemCell Technologies, #19844A) following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 250,000-500,000 CD4+ T cells per well were plated in 96-well V-bottom plates ...
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bioRxiv - Immunology 2021Quote: Splenic naïve B lymphocytes were isolated from WT mice by magnetic bead negative selection using EasySep Mouse B Cell Isolation Kit (Catalog no. 19854) from STEMCELL Technologies (Vancouver, BC). B cells were cultured in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2021Quote: Neuroectodermal organoids were prepared using hiPS cells expressing OptoShroom3 following the first steps of cerebral organoid protocol53 with STEMdiff™ Cerebral Organoid Kit (STEMCELL Technologies, #08570). The modifications from commercial protocol were ...
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bioRxiv - Biophysics 2022Quote: ... Human neutrophils were isolated from whole blood of healthy donors by immunomagnetic negative selection using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Isolation began from an initial blood volume of 0.5-1 ml ...
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bioRxiv - Immunology 2019Quote: ... CD4+ cells were separately isolated using mouse CD4+ T-cell negative selection protocol (EasySep™ Mouse CD4+ T-cell Enrichment Kit, Stem Cell Technologies) to a purity of > 90% according to the manufactureŕs instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Initial HSPC isolation was performed using defined protocols of EasySep™ Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (#19756, #18000, Stemcell Technologies, CA). A secondary isolation further enriched the HSPC population by collecting the Lin− Sca-1+ c-kit+ (LSK ...