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Citations for Stemcell Technologies :
1601 - 1650 of 4551 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Six-well plates coated for at least 2h with 10µg/mL of Cell Adhere Laminin 521 (Stem Cell Technology, 200-0117) diluted in DPBS containing calcium and magnesium (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... enriched MDA-MB-231 cell line cells or CTCs were stained for EpCAM (1:50, VU1D9, Stemcell Technologies, Vancouver, Canada, AF488) and CD45 (1:25 ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of naïve (CD44loCD62Lhi) lymph node CD8+ T cells (isolated using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit, Stemcell Technologies) were washed with ice-cold PBS supplemented with 2% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were either harvested to assess levels of exhaustion marker expression by flow cytometry or DMSO-treated cells were purified using the mouse CD8 T cell isolation kit (StemCell Technologies). Enriched mouse CD8+ T cells were then treated with either DMSO alone or soquelitinib at concentrations ranging from 0 to 10 μM with additional two rounds of stimulation with the OVA peptide ...
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bioRxiv - Physiology 2023Quote: ... Cells were then plated between 450,000 - 750,000 cells per well of a Matrigel coated 12-well tissue culture plate in mTeSR1 PLUS™ (Stem Cell Technologies) supplemented with 10 µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Molecular Biology 2023Quote: CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). CD4+ cells were then immediately used for bulk ATAC-seq ...
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bioRxiv - Bioengineering 2023Quote: ... Human T cells were purified (> 96% purity) by magnetic negative selection using an EasySep Human T Cell Isolation Kit (Cat#17951, Stemcell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... For microglial differentiation iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (05310, Stem cell Technologies). HPCs were either frozen using Bambanker HRM freezing media (BBH01 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary human CD3+ T cells were isolated from PBMCs by negative selection using EasySep Human T Cell Isolation Kit (StemCell Technologies) or by positive selection using Dynabeads Human T-Expander CD3/CD28 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse T-cells were isolated from mouse PP/lymph nodes/spleens and tumors using EasySep Mouse CD8+ T Cell Isolation kit (StemCell Technologies). For FACS analysis ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... All co-cultured cells were collected after trypsinization and then plated to duplicate dishes in MethoCult medium (Stem Cell Technologies, H4534) supplemented with 3 units/ml of human EPO (Peprotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells were lysed using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... Criteria included hESC stemness of at least 80% of double positive cells for both stemness markers SSEA-1 (Stem Cell Technologies) and TRA-1-60 (Stem Cell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were isolated from PBMCs using an EasySep Human CD4+ T Cell Isolation Kit (17952, StemCell Technologies, Canada) as per manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1ml of the mixture (2 x 104 BM cells and 2 x 105 spleen cells) was plated in triplicate into 6-well dishes (SmartDishTM, STEMCELL Technology). After incubation for 10 days ...
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bioRxiv - Bioengineering 2023Quote: ... was prepared by mixing equal volumes of 3mg/mL neutralized collagen with 10% Geltrex and 8×107 cells/mL cell suspension in mTeSR 1(STEMCELL Technologies) containing 10μM ROCK inhibitor Y27632 (Cayman) ...
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bioRxiv - Neuroscience 2024Quote: ... or at a density of 5.0 x 104 cells/cm2 on Laminin-/Poly-L-Ornithine coated plates in StemDiff Forebrain Neuron Maturation medium (Stem Cell Technologies) supplemented with 1x Pen/Strep ...
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bioRxiv - Neuroscience 2024Quote: ... cryo-vials of neural progenitor cells were thawed and cells were seeded at a density of 1.25 x 105 cells/cm2 on Matrigel-coated dishes in StemDiff Neural Progenitor medium (Stem Cell Technologies) on day 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... and SK-ES-1 cells were grown and dissociated as above and cells were resuspended in MesenCult MSC basal media (Stemcell Technologies) supplemented with MesenCult MSC Stimulatory Supplement (Human ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ CD45RO+ memory T cells were then isolated by negative selection using an EasySepTM Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies).
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purified from healthy donor blood samples using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies). For cloning ...
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bioRxiv - Cell Biology 2024Quote: ... The human primed iPSC lines as well as human primed ES cell line H9 (#WA09, obtained from WiCell Research Institute) were maintained without feeder cells either in mTeSR1 (STEMCELL Technologies) on growth factor reduced Matrigel (Corning ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... YS suspension or sorted cells were plated in triplicate (3×103 or 100-150 cells/mL) in Methocult® M3234 (StemCell Technologies) always supplemented the cytokines listed in supplemental table 5 ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cell growth monitoring and analysis of hESCs trilineage differentiation were performed using the Cell Counting Kit-8 (Dojindo) and STEMdiffTM trilineage differentiation kits (Stem Cell Technology), according to the manufacture’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
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bioRxiv - Neuroscience 2019Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... all the cells transfected with Sendai virus were transferred into a 6-well plate (30,000 cells per well) each well containing ~150,000 mouse embryonic fibroblast (MEF) feeder cells (Stemcell Technologies Inc. Vancouver, Canada). The MEF plate preparation was conducted the day before plating the reprogramming cells as explained elsewhere (28) ...
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bioRxiv - Cancer Biology 2019Quote: ... Low density CD3-CD19-CD11b-cells simultaneously depleted of neutrophils and red cells were isolated on Lymphoprep using RosetteSep™ and the >90% pure CD34+ cells isolated using EasySep (both STEMCELL Technologies). Cells were stored frozen at −176 °C in DMSO with 90% fetal bovine serum (FBS) ...
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bioRxiv - Genetics 2019Quote: Umbilical cord blood Lineage negative (Lin-) and CD34+ cells were obtained using StemSep system according to the manufacturer’s protocol (Stem Cell Technologies, Canada). Lin-CD34+ cells were sorted to obtain HSC (CD34+38-/lowCD45RA-CD90+) ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated from whole blood using ficoll density gradient centrifugation in a SepMate tube (Stem Cell Technologies) following 1:2 dilution with RPMI ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells from healthy donors were purified from buffy coats purchased from Research Blood Components or leukopaks purchased from Stem Cell Technologies using Ficoll-Paque PLUS (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice a week using an enzyme-free dissociation reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... we thawed frozen viable breast cancer pleural effusions or ascites from patients and depleted dead cells with EasySep™ Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies) and CD45+ cells with EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: Between 250-500 CD34+ cells were directly sorted into 250 µL of enriched methylcellulose (Methocult H4435 Medium, Stem Cell Technologies, Vancouver, Canada), and then plated into p35 culture dishes containing a final volume of 1.1 mL of enriched methylcellulose ...
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bioRxiv - Cancer Biology 2020Quote: ... washed with PBS twice to eliminate the PBMCs not inside the organoids and incubated with Gentle Cell Dissociation reagent (Stem Cell Technologies) for 2 min at RT and mechanically disrupted by pipetting ...
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bioRxiv - Genomics 2021Quote: ... were sorted using a BD FACSAria™ Fusion Cell Sorter into ice-cold Serum-Free Expansion Medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies), 20 ng/ml IL-3 ...
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coat (Research Blood Components) using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Neuroscience 2021Quote: ... the corrected and patient cell lines were maintained in plates coated with Geltrex (1:50 DMEM/F12) overnight using mTeSR (Stem Cell technologies). The media was refreshed daily for iPSCs ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
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bioRxiv - Cancer Biology 2020Quote: ... Appropriate numbers of total bone marrow cells were then exposed to various concentration of MMC and seeded into 6-well plates with MethoCult GF M3434 (Stem Cell Technologies) media following the manufacturer’s instructions.