Labshake search
Citations for Stemcell Technologies :
1551 - 1600 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... The cells were cultured in Mammocult media supplemented with 4 μg/ml Heparin (Stem Cell Technology), 0.5 μg/ml hydrocortisone and 10% Mammocult Proliferation Supplement (Stem Cell Technology) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were adjusted to 1×105 cells/ml final concentration in H4230 methylcellulose medium (STEMCELL Technologies) supplemented with 10% 5637-CM as a stimulant and then plated in 35-mm petri dishes in duplicate and allowed to grow for 7 days in a humidified CO2 incubator (5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: Cells were dissociated from the well using Gentle Cell Dissociation reagent (StemCell Technologies, Cat#: 100-0485). Total proteins were extracted by adding 1ml of cold 10% TCA to the cell pellets ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell colonies were separately dissociated and transferred into 6-well plates using ReLeSR (Stem Cell Technologies). Differentiated cells were removed by using a pipette tip to minimize spontaneous differentiation ...
-
bioRxiv - Microbiology 2020Quote: ... CD45− cells were magnetically sorted using Ter119/CD45-depletion kit (#19771; Stem Cell Technologies, Vancouver, BC). Next ...
-
bioRxiv - Microbiology 2021Quote: ... and stem cells were passaged using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech#05872). Cultures were maintained in an undifferentiated state ...
-
bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2020Quote: ... bulk CD4+ T cells were isolated using the EasySep CD4+ T cell Enrichment Kit (StemCell Technologies). Isolated CD4+ T cells were maintained in RPMI medium supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin (R10 medium) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Then cells were re-suspended in 500μl and counted in 500μl ammonium chloride (Stem Cell Technologies) using the Vicell XR (Beckman Coulter) ...
-
bioRxiv - Developmental Biology 2022Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies) with 2 μM ROCK inhibitor (Thiazovivin/TZV ...
-
bioRxiv - Cancer Biology 2022Quote: Bone marrow cells were subjected to RBC lysis using Ammonium Chloride lysis buffer (Stem Cell Technologies), then incubated with anti-mouse direct lineage depletion cocktail (Miltenyi ...
-
bioRxiv - Immunology 2022Quote: NK cells from spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434; Stem Cell Technologies) at 1000 cells/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... Medium was changed every day and cells were passaged using gentle cell dissociation reagent (Stemcell Technologies) or singularised using accutase (Stem Cell Technologies) ...
-
bioRxiv - Immunology 2022Quote: ... CD8 T cells were enriched using an EasySep magnetic negative selection kit (Stem Cell Technologies #19853) as per the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
-
bioRxiv - Cancer Biology 2022Quote: ... RBC-lysed 50,000 whole bone marrow cells were seeded in 1.5mL MethoCult M3434 (Stem Cell Technologies) with no additional supplemental cytokines in triplicate on 6 well plates and scored on day 8 ...
-
bioRxiv - Bioengineering 2020Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies).
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were then treated with 2ml of 0.25% Trypsin/EDTA solution (Stem Cell Technologies, cat #07901) and 2ml of 5mg/mL dispase (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... PE positive cells were enriched using an EasyEights™ magnet (Stem Cell Technologies, Cat no. 18103). Three rounds of separation were performed in total and cells were washed in complete media prior to maintenance in culture with complete RPMI + IL-2 ...
-
bioRxiv - Immunology 2021Quote: ... EasySep Mouse Naive CD4+ T cell and CD8+ T cell Isolation Kit (StemCell Technologies, Vancouer, Canada) were purchased.
-
bioRxiv - Cell Biology 2020Quote: ... Single cell suspensions were enriched for NK lineage cells using a bivalent antibody RosetteSep (StemCell Technologies)-based method (55) ...
-
bioRxiv - Cell Biology 2021Quote: ... CD34+ cells (1 × 105/mL) were suspended in serum-free expansion medium II (Stem Cell Technologies) with 50 ng/mL stem cell factor (PeproTech ...
-
bioRxiv - Neuroscience 2021Quote: ... hiPSCs were detached from the Vitronectin XF substrate using Gentle Cell Dissociation Reagent (Stem Cell Technologies), centrifuged ...
-
bioRxiv - Immunology 2021Quote: ... CD3+ T cells or CD4+ T cells were isolated via Lymphoprep and RosetteSep (both STEMCELL Technologies). For Treg isolation ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed in PBS and incubated in PBS + 50 nM dasatinib (Stem Cell Technologies, Canada) + DNAse at 37°C for 30 min ...
-
bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells from healthy donors were purified from leukopaks purchased from Stem Cell Technologies using Ficoll-Paque PLUS (GE Healthcare ...
-
bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were prepared by treatment of hES cells with accutase (#07920, StemCell Technologies Inc.) and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307 ...
-
bioRxiv - Physiology 2023Quote: ... Cells were washed with 1x PBS and plated in methocullulose media (Methocult M3434, Stem Cell Technologies). Cells were plated (4 × 105 cells per 35 mm-tissue culture dish ...
-
bioRxiv - Immunology 2023Quote: ... Dendritic cells were isolated with the EasySepTM Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies). T Cells were isolated with the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies) ...
-
bioRxiv - Immunology 2024Quote: ... CD8+ T cells were isolated using the EasySep Mouse CD8+ T cell isolation kit (StemCell Technologies) and resuspended at a concentration of 106 cells/mL in complete RPMI supplemented with 1X sodium pyruvate (ThermoFisher) ...
-
bioRxiv - Immunology 2024Quote: ... T cells were isolated from PMBCs using a Nonhuman Primate T-Cell Isolation Kit (STEMCELL Technologies), activated with a nonhuman primate T-Cell Activation/Expansion Kit (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2024Quote: ... Cell clusters floating in suspension were dissociated into single-cells with Accutase (Stemcell technologies, Cambridge, MA). The single cell population was resuspended in their respective NDM/GDM media supplemented with RI (StemGent ...
-
bioRxiv - Neuroscience 2024Quote: ... GBM cells were grown in serum-free Neurocult NS-A Basal medium (Stem Cell Technologies #05750) that is supplemented with 2mM L-Glutamine (Wisent #609—65-EL) ...
-
bioRxiv - Cell Biology 2023Quote: ... All MSCs were cultured on ACF Cell Attachment Substrate coated surfaces (Stem Cell Technologies; Catalogue #07130). ACF Cell Dissociation Kit (Stem Cell Technologies ...
-
bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells from healthy donors were purified from leukopaks purchased from Stem Cell Technologies using Ficoll EasySep Direct Human PBMC Isolation Kits (Stem Cell Technologies ...
-
bioRxiv - Cell Biology 2023Quote: ... after which ALI was induced and cells maintained in PneumaCult™-ALI media (Stem Cell Technologies).
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were treated overnight with 10 µM Rock inhibitor Y-27632 (Cat. # 72304, Stem Cell Technologies) to attenuate cell death ...
-
bioRxiv - Cell Biology 2023Quote: ... the EBs were separated from loose cells using a reversible strainer (Stem Cell Technologies Cat# 27259) and transferred to a new ultralow attachment plate with EB media (Stem Cell Technologies Cat# 05893) ...
-
bioRxiv - Bioengineering 2023Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies). Accutase (Innovative Cell Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... The CD34+ cells were purified from PBMNCs by the magnetic separation method (EasySep, Stem Cell Technologies) and were cryopreserved ...
-
bioRxiv - Neuroscience 2023Quote: ... Single clones were generated by seeding single cells in presence of CloneR (05888, Stem Cell Tech). All clones and the bulk population were routinely subjected to mycoplasma testing ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell clusters were released from Matrigel by incubation in dispase 5U/mL (Stem Cell Technologies 07913) for up to 30 minutes at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... HUDEP-2 cells were expanded in a basal medium composed of StemSpan SFEM (Stem Cell Technologies) supplemented with 10−6 M dexamethasone (Sigma) ...
-
bioRxiv - Immunology 2022Quote: ... The cells were then transduced with barcode lentivirus library (31) in StemSpan medium (Stem Cell Technologies) by centrifugation at 900 ×g for 1.5h at room temp ...
-
bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were then reaggregated in low adhesion microwell culture plates (AggreWell-800, Stem cell technologies). 3 million cells were plated per 2mL well in mTesR plus Y-27632 (1 mM ...
-
bioRxiv - Neuroscience 2023Quote: ... Medium was changed every day and cells were passaged using gentle cell dissociation reagent (Stemcell Technologies). Differentiation of hiPSCs to glutamatergic neurons was based on a modified method of Chambers et al ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
-
bioRxiv - Immunology 2023Quote: ... Neutrophil and monocyte cell population were each obtained by negative selection (>97% purity, Stem Cell Technologies). To generate human monocyte-derived macrophages ...