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Citations for Stemcell Technologies :
1551 - 1600 of 1811 citations for Human Suprabasin SBSN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
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bioRxiv - Cell Biology 2023Quote: ... The timely collection of the CMs was done using STEMdiff reagent from the CM dissociation kit (STEMCELL Technologies, Vancouver, BC). STEMdiff was added to each well of hiPSC-CMs ...
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bioRxiv - Bioengineering 2023Quote: Organoids were dissociated into a single-celled suspension using a modified protocol using the STEMdiff Cardiomyocyte Dissociation Kit (STEMCELL Technologies). Upon being transferred to a microcentrifuge tube ...
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bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD8+ Tn or Teff cells isolated from LmOVA-infected mice by Thy1.1 positive selection kit (StemCell Technologies, Vancouver, BC, Canada) and were cultured immediately ex vivo in Seahorse XF medium (non-buffered DMEM 1640 containing 25mM glucose ...
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bioRxiv - Immunology 2023Quote: ... and cells were counted using a hemocytometer and dead cells were removed with a magnetic bead based dead cell removal kit according to the manufacturer’s instructions (EasySep STEMCELL technologies). Following lung digestion 1/4 of the cell suspension was sorted for CD45+ CD3- CD19- Ly6G- FceRIa- CD5- NK1.1- CD127+ CD90+ ILCs and spiked back into the whole lung single cell solution ...
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bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from PBMC by depletion of non-NK cells using an NK cell isolation kit (STEMCELL Technologies). The manufacturer’s protocol was modified as follows ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Developmental Biology 2023Quote: ... Clones with the insertions were then tested for common chromosomal abnormalities using the hPSC Genetic Analysis Kit (STEMCELL Technologies Inc.).
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Developmental Biology 2023Quote: ... the H1 ES cell line was differentiated to PP cells using the STEMdiff™ Pancreatic Progenitor Kit (STEMCELL Technologies, 05120) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Dorsal forebrain regionalized neural organoids (RNOs) were generated using the STEMDiff™ Dorsal Forebrain Organoid Differentiation Kit (STEMCELL Technologies™) and STEMDiff™ Neural Organoid Maintenance Kit (STEMCELL Technologies™ ...
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bioRxiv - Genetics 2023Quote: The generated iPSCs were differentiated into three germ layers using the STEMdiffTM Trilineage Differentiation Kit (Stem Cell Technologies Catalog #05230).
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bioRxiv - Genetics 2023Quote: The differentiation of hESCs toward beating cardiomyocyte was performed following STEMdiffTM Ventricular Cardiomyocyte Differentiation Kit (Stem Cell Technologies Catalog #05010) in accordance with the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated into single-cell suspension using Accutase and re-seeded onto fresh Poly-L-Ornithine / Laminin coated 12-well plates at a density of 150,000 cells in STEMdiff Neuron Maturation Kit medium (Stemcell Technologies) and medium exchanged every other day until days 14 and 21.
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Frozen cells were thawed at 37°C and treated using the ErythroClear™ Red Blood Cell Depletion Kit (STEMCELL Technologies). Cells were constructed into barcoded single-cell cDNA libraries and sequenced as described above.
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bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
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bioRxiv - Cell Biology 2019Quote: ... at a density of 9000 cells per well in 100 μl of Embryoid Body Formation Medium (STEMdiff Cerebral organoid Kit, STEMCELL Technologies) with either addition of 10 μM Y-27632 (Tocris ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from the spleens of anemic mice using the EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... cardioids were maintained in CDM with insulin.21,31 Samples were collected 7 days post induction and dissociated using the STEMdiff cardiomyocyte dissociation kit (#05025, Stem Cell Technologies) and subsequently washed 3x with PBS.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... NK cells were isolated from buffy coats from healthy donors using the RosetteSep NK cell enrichment kit (STEMCELL Technologies, MA, USA) and stimulated with rhIL-15 (1ng/ml ...
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bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...
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bioRxiv - Immunology 2019Quote: OT1 and OTII T cells were isolated from LN of TCR transgenic mice using either a CD8 or CD4 StemSep enrichment kit (STEMCELL Technologies), respectively ...
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bioRxiv - Cancer Biology 2019Quote: Mouse CD3 positive T cells were isolated from spleens collected from CEA transgenic mice using negative selection kit (Stemcell Technologies, Canada) and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... were thawed at 37°C and dead cells were removed through Annexin V beads-mediated selection (EasySep Dead Cell Removal Annexin V Kit, Stemcell technology). CD4+ T cells were further isolated through beads-mediated negative selection (EasySep Human CD4+ T cell Isolation Kit ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated in EasySep FITC/PE Positive Selection kit which labels FITC/PE epitopes with magnetic beads (Stemcell Technologies 18557, 18555). Each step was followed by a wash and spin ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...
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bioRxiv - Cell Biology 2021Quote: ... Magnetic depletion of lineage-positive haematopoietic cells was performed using the EasySep mouse haematopoietic progenitor cell enrichment kit (Stem Cell Technologies), and lineage-depleted stem and progenitor cells were submitted to mass spectrometry analysis ...
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bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... Human pluripotent stem cell (hPSC)-derived cerebral organoids were grown at 37°C with 5% CO2 in STEMdiff Cerebral Organoid Kit cell culture media from STEMCELL Technologies Inc ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from healthy adult PBMC using a RosetteSep NK Cell Enrichment Kit (Stem Cell Technologies, Vancouver, BC, Canada), according to manufacturer’s instructions followed by density gradient centrifugation in Histopaque ...
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bioRxiv - Molecular Biology 2020Quote: ... and LN cells were enriched for CD4+ T cells using the EasySep NHP CD4+ T cell isolation kit from STEMCELL Technologies Canada Inc (Cat# ...
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bioRxiv - Microbiology 2020Quote: ... DCs and naïve CD4+ T cells were harvested from SPLNs of mice at 7 weeks of age using a CD11c positive selection kit II (STEMCELL Technologies) and a mouse naïve CD4+ T cell Isolation kit (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Lin−Sca-1+c-Kit+CD150+CD48− (HSC) or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) populations were cultured in Myelocult M5300 (STEMCELL Technologies) with 100 µg/ml Primocin (Invivogen ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged and dissociated as described above for EB generation into single cells and cultured on Matrigel-coated plates with the STEMdiff Trilineage differentiation kit (used according to the manufacturer’s instructions, Stemcell Technologies, 05230). Cells were harvested at the indicated day for gene expression analysis by qPCR.
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bioRxiv - Immunology 2022Quote: Neutrophils were obtained from the bone marrow of C57BL/6 wild-type mice using the EasySep Mouse Enrichment Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... total B cells from the spleens and lymph nodes were isolated using the Negative Selection B cell isolation kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...