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Citations for Stemcell Technologies :
1551 - 1600 of 1660 citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 500 of sorted lentivirally transduced CD34+ HSPCs were seeded into 1 mL of MethoCultTM H4434 Classic media (STEMCELL Technologies #04434) supplemented with StemSpan™ CC100 with antibiotics ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies, Vancouver, BC, Canada) for 24 days ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies, Vancouver, BC, Canada) for 24 days ...
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bioRxiv - Bioengineering 2023Quote: ... Embryoid bodies formed via hanging drop approach with each droplet containing 3-4×104 human single PSCs in 20 μl were cultured hanging on TESR-E8 supplemented with Polyvinyl Alcohol (PVA) at 4mg/ml (SigmaAldrich) and rock inhibitor (Y-27632) at 10μM (StemCell Technologies) at the lid of Petri dishes ...
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bioRxiv - Developmental Biology 2023Quote: ... 1x penicillin-streptomycin and 10ng/ml FGF2 (Department of Biochemistry, University of Cambridge) and 10µM ROCK inhibitor Y-27632 (72304, STEMCELL Technologies). For RSeT cells ...
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bioRxiv - Cell Biology 2022Quote: ... The diluted blood was layered on top of 15 mL Lymphoprep™ density gradient medium (StemCell Technologies Inc., Vancouver, Canada) in a 50 mL Falcon tube ...
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bioRxiv - Genomics 2022Quote: ... An addition 1.65 mL of homogenization buffer was topped up and mixed with 2.65 mL of gradient medium (5mM CaCl2, 50% Optiprep (Stemcell Technologies), 3mM Magnesium Acetate ...
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bioRxiv - Immunology 2023Quote: ... uncoagulated whole blood from male and female donors (age range: 21-66) was spun at 1,200 × g in 50-mL SepMate tubes (STEMCELL Technologies) containing Ficoll-Paque PLUS (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 H3.1-K27M (GSC3, GSC4) cellular models were grown in NeurocultTM medium supplemented with heparin (2 μg/mL, Stemcell Technologies), EGF/FGF (20ng/mL ...
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bioRxiv - Bioengineering 2024Quote: ... 5% CO2 for 48 hours prior to electroporation at a seeding concentration of 4.0 x 105 cells/mL in HSPC culture media (StemSpan SFEM II (StemCell Technologies) supplemented with penicillin/streptomycin (100 U/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... with each droplet containing 4×103 human single PSCs in 20 μl were cultured hanging on TESR-E8 supplemented with Polyvinyl Alcohol (PVA) at 4mg/ml (SigmaAldrich) and rock inhibitor (Y-27632) at 10μM (StemCell Technologies) at the lid of Petri dishes ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were resuspended gently in 0.5 mL RPMI media before following the negative selection EasySep™ Mouse Neutrophil Enrichment Kit (StemCell Technologies). Neutrophils were labeled with 5 μM CellTracker (CFMDA ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Genetics 2019Quote: ... Following six PBS platelet removal washes these were resuspended at 1×106 cells/ml in phase one media: Stemspan medium (Stemcell Tech), 1μg /ml cyclosporin A (Sigma) ...
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bioRxiv - Bioengineering 2020Quote: ... Clear supernatant was removed and intestinal pieces were resuspended in 25 ml of Gentle Cell Dissociation Reagent (Stem Cell Technologies, 07174). Tubes were incubated at room temperature on a rocking platform for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were preactivated for 5 days with IL-2 and either 1:1 aAPCs or 25 μl/ml CD3/CD28/CD2 tetramer (ImmunoCult Human CD3/CD28/CD2 T Cell Activator, STEMCELL Technologies). L cells (ATCC CRL-2648 ...
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bioRxiv - Bioengineering 2020Quote: ... After 72 hours the medium was replaced with 7 mL of neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 nM and smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2020Quote: ... at 2.5 × 105 cells/well and topped off with 1 mL of either EpiCult™ Medium (StemCell Technologies, cat. no. 05610) supplemented with 5% FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... single hESCs were seeded into a spinner flask at a density of 1e6 cells/mL in mTeSR1 media containing 10 μM Rock inhibitor Y-27632 (STEMCELL Technologies) to allow formation of clusters ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed in IMDM+20%FCS and plated into 1.4 mL of cytokine-rich methylcellulose (Methocult H4435, Stem Cell Technologies). Colonies were scored 14 days later under an inverted microscope ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... On days 8-15+ cell culture media was exchanged every 2 days with 15 mL of Cardiomyocyte Maintenance Medium (STEMCELL Technologies). On day 30 ...
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bioRxiv - Developmental Biology 2022Quote: ... medium was replaced by 1 ml of a 1:1 mixture of UPC medium and reprogramming medium TeSR-E7 (STEMCELL Technologies). From day 4 to day 18-20 ...
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bioRxiv - Bioengineering 2019Quote: ... and an “activated” population that was cultured in medium supplemented with 25 μl/ml tetrameric antibody against CD2/CD3/CD28 (StemCell Technologies). Quiescent and activated T cell populations were cultured separately for 48 hours at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... and cultured at a concentration of 1×103 cells mL−1 in one well of a 6-well plate (#1008; Becton-Dickinson) with 1 mL MethoCult GF+ semisolid medium (#4435, STEMCELL Technologies). Colonies were counted after 14 days of incubation using an inverted microscope by three individuals.
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bioRxiv - Cell Biology 2022Quote: CFU assays in PHZ-treated and retrovirally-infected spleen progenitors were conducted using spleen suspensions at 2 × 104 cells/mL mixed with Methocult M3434 (STEMCELL Technologies) containing Epo ...
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bioRxiv - Neuroscience 2021Quote: ... placed in a stereotaxic frame and injected with either 100,000 hNSCs per side (2 μl/injection) or vehicle (2 μl HBSS with 20 ng/ml epidermal growth factor [STEMCELL Technologies, #78003] and human fibroblast growth factor [STEMCELL ...
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bioRxiv - Cell Biology 2019Quote: ... The gathered crypts were centrifuged at 1200 rpm for five minutes and digested with dispase (1 U/ml, Stem Cell Technologies). The single cell suspension was passed through a 40µm cell strainer (BD biosciences ...
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bioRxiv - Cancer Biology 2021Quote: Tumor tissue excised from the brain was mechanically dissociated using a 70 um cell strainer in phosphate-buffered saline (PBS) with 10% FBS and 0.1 mg/mL DNAse I (Stem Cell Technologies). Cells were then resuspended in 25% isotonic Percoll solution and centrifuged for 20 minutes at 18°C with acceleration and deceleration set to minimum ...
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bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... or were cultured for 48 hours at a density of 2.5 × 105 cells/ml in StemSpan SFEM II (STEMCELL Technologies Inc.) supplemented with Stem Cell Factor (SCF ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
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bioRxiv - Microbiology 2024Quote: ... was added to the blood sample before transferring to SepMate TM tubes prefilled with 4.5 mL of Lymphoprep (STEMCELL Technologies, Canada) density gradient medium ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were dissociated into single cells using Accutase and plated on 10 µg/mL Vitronectin XF (Cat. No. 07180, STEMCELL Technologies)-coated plates with 10 µM of Y27632 (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 0.5-1 x 107 cells/ml in “phase 1 expansion medium” consisting of Stemspan SFEM (Stem Cell Technologies) supplemented with Stem Cell Factor (SCF ...
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bioRxiv - Cell Biology 2024Quote: ... were washed three times with PBS and incubated for 30 minutes at 37 °C with 60 ng/mL DNAse I (Stemcell technologies) in DNAse buffer solution (Table 1) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were centrifuged at 200 x g for 2 min and resuspended in neural media (NM; Supplementary Table 2) supplemented with 0.02 µg/mL FGF2 (StemCell Technologies, #78003) and 0.02 µg/mL EGF (StemCell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Immunology 2024Quote: ... were thawed on the day of the experiment and plated in each well supplemented with 50 ng/mL IFNγ (STEMCELL Technologies) for overnight activation ...
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bioRxiv - Cell Biology 2023Quote: ... The mTeSR-1 cell medium (was replaced on a daily basis thereafter and the iPSC colonies were subcultured every seven days using 1 U/ml Dispase (Stemcell Technologies). During cultivation ...
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bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Peripheral Blood Mononuclear Cells (PMBCs) were isolated from the buffy coat of whole blood (20 mL) using RosetteSep human enrichment cocktail (STEMCELL Technologies) and density gradient centrifugation as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 550,000 neurons were seeded in 6 well plates coated with 1 mg/mL polyLysine were cultured in conditioned Neurobasal medium supplemented with 2 mM L-glutamine and NeuroCult SM1 Neuronal supplement (STEMCELL Technologies) half renewed every 3 days with 3.4mM AraC ...
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bioRxiv - Genetics 2023Quote: ... the cells were washed with DMEM/F12 and then incubated for ∼1.5 hours with 1 mL of STEMdiff Neural Rosette Selection Reagent (STEMCELL Technologies, #05832) in each well ...
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bioRxiv - Neuroscience 2023Quote: ... Matrigel was diluted according to the lot-specific dilution factor recommended by the manufacturer in 25 ml of DMEM/F12 HEPES (Cat No. 36254, STEMCELL Technologies) and set for 1 hour at 37°C before plates were used ...