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Citations for Stemcell Technologies :
1501 - 1526 of 1526 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 mL of cell suspension was added to each well of a 12-well Collagen I-coated plate (STEMCELL Technologies, #100-0363). Cells were allowed to settle overnight in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2024Quote: ... and then re-seeded in supplemented mTeSR1 medium with 1% P/S and a 10 μM concentration of the ROCK inhibitor Y-27632 (STEMCELL Technologies, Cat. # 72308). A comprehensive protocol is available at https://www.allencell.org/.
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bioRxiv - Immunology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy Sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy Sep direct human monocyte isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and then re-seeded in supplemented mTeSR1 medium with 1% penicillin–streptomycin and a 10 μM concentration of the ROCK inhibitor Y-27632 (Stem Cell Technologies, 72308). Further details for cell culture reagents and consumables can be found in the Resource Table and standard protocols can be found at www.allencell.org.
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bioRxiv - Neuroscience 2024Quote: Maintenance of iPSC lines (C4 healthy control and DJ-1 deficient C4 mut line) was done in mTeSR™ Plus medium (Stem Cell Technologies). To achieve microglia differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... ESCs were fed daily and were passaged every 3-4 days at a 1:6 ratio using ReLeSR™ (STEMCELL Technologies, #100-0483) following manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... HPNE cells were grown in a 3:1 mixture of DMEM+GlutaMAX: Medium M3 Base supplemented with 10% FBS and 10 ng/ml human recombinant EGF (StemCell Technologies Inc. 78006). HUVEC and TeloHAEC derived cell lines were grown in Vascular Cell Basal Medium supplemented with the Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041) ...
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bioRxiv - Bioengineering 2020Quote: MSCs at passages 1 or 3 were seeded in wells of a 12-well plate containing 3 ml Mesencult (Stem Cell Technologies, Vancouver, B.C.) each at 8.0×104 ...
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
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bioRxiv - Neuroscience 2023Quote: ... Daily media changes of E7™ Reprogramming Media followed until the emergence of iPSC colonies between day 21-28 when iPSC colonies were picked and transferred to Geltrex™ coated 12-well plates containing 1 mL TeSR™-E8™ (STEMCELL Technologies) supplemented with 10 μM ROCK Inhibitor (Y-27632 ...
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bioRxiv - Genetics 2024Quote: ... On day 10 the cells were frozen down into matrix tubes at a density of 1 million cells per vial using the CryoStor® freezing medium (Stemcell Technologies, #100-1061) (Figure 5A).
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bioRxiv - Cell Biology 2024Quote: ... and the Oregon Green positive cells were sorted into 1 cell/96-well in Matrigel-coated 96-well plate with 1x CloneR2 (Stem Cell Technologies, # 100-0691) and 100µL/well StemFlex using BD FASCMelody cell sorter.
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bioRxiv - Cancer Biology 2024Quote: ... cells were changed to RPMI phenol red-free + 5% charcoal-stripped serum (CSS) and grown in the DMSO and ethanol or 1 mM dibutyryl cyclic-AMP (db-cAMP, STEMCELL Technologies, Vancouver, BC, Canada) and 0.5 mM 3-Isobutyl-1-methylxanthine ...
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bioRxiv - Cell Biology 2023Quote: ... and the picked colonies were allowed to grow larger and they ‘passaged’ by mechanical splitting into a new Matrigel coated plate containing mTeSR™1 media with its supplement(Anonymous, n.d.) and maintained as suggested by the manufacturer (StemCell Technologies, Canada Cat. No. #85850). Subsequent passages were done by enzymatic dissociation (Accutase® ...
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bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
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bioRxiv - Biochemistry 2024Quote: ... 5 ml of crude cell extracts (CCE) of constructs overproducing the citrine-ferritin fusions were mixed with 1 ml of OptiPrep Density Gradient Medium (STEMCELL Technologies Germany GmbH, Köln, Germany) and transferred to mini petri dishes ...
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bioRxiv - Cell Biology 2022Quote: WIBR3 (NIHhESC-10-0079) hESCs and isogenic LIS1+/- 10 were grown and maintained on irradiated MEFs in optimal naive NHSM conditions (RSET-Stem Cell Technologies INC, supplementary Table 1) 90 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies, Vancouver, BC, Canada).
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cells from each experimental group were transferred to a single 35 mm culture dish containing 1 mL of MethoCult GF M3534 culture medium (Methylcellulose medium with recombinant cytokines for mouse cells, Stem Cell Technologies, Vancouver, CA, catalog no. 03534) using a 3 mL syringe and 16-gauge needle ...
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bioRxiv - Developmental Biology 2023Quote: ... Explants were then cultured in suspension in DMEM for 2 hours at 37°C with a 1/250 dilution from 100 mM stock solution or Dispase II (Stem Cell Technologies; #07923, at 0.2 U/mL).
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bioRxiv - Developmental Biology 2024Quote: ... it was switched to PXGL medium (N2B27 medium supplemented with 1 μM PD0325901, 2 μM XAV939 [STEMCELL Technologies, 72674], 2 μM Gö6983 [STEMCELL Technologies, 72462], and 10 ng/mL LIF), refreshed daily for an additional 8-10 days before passaging ...