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Citations for Stemcell Technologies :
1451 - 1500 of 1527 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... was prepared by mixing equal volumes of 3mg/mL neutralized collagen with 10% Geltrex and 8×107 cells/mL cell suspension in mTeSR 1(STEMCELL Technologies) containing 10μM ROCK inhibitor Y27632 (Cayman) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 106 lineage negative cells/ml were transduced with 1 x 108 TU/ml of empty vector or Vegfc lentivirus in serum-free Stem-Span medium (StemCell Technologies) supplemented with 10 ng/ml IL-3 ...
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bioRxiv - Cancer Biology 2024Quote: ... and SK-ES-1 cells were grown and dissociated as above and cells were resuspended in MesenCult MSC basal media (Stemcell Technologies) supplemented with MesenCult MSC Stimulatory Supplement (Human ...
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bioRxiv - Cell Biology 2024Quote: ... For ECM concentration experiments and mechanical actuation experiments on Mechanoculture FX2 plates fibronectin coating (1 µg/mL and 10 µg/mL, in PBS StemCell Technologies) at 37°C for 1 hour were used.
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bioRxiv - Molecular Biology 2024Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then plated at 25 cell/cm2 on 2x Cultrex-coated plates in mTeSR-Plus media with 1× CloneR supplement (STEMCELL Technologies). Plates were incubated for 2 days before changing the media to mTeSR-Plus ...
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bioRxiv - Bioengineering 2024Quote: ... 1 × 104 cells/mL CD34+ cells were plated in 35 mm cell culture dishes in 1 mL Methocult H4230 medium (Stemcell Technologies) supplemented with 2% FBS ...
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bioRxiv - Synthetic Biology 2024Quote: H1 & H9 Pansio-1 lines targeted with different BxbI expressing modalities and pMIA10.53-copGFP donor were disassociated with accutase (STEMCELL Technologies, 07922) and resuspended in PBS ...
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bioRxiv - Cancer Biology 2021Quote: Ten thousand cells of RPMI-2650 and Detroit celllines were seeded in 24-well Ul-tra-Low Attachment Plates (Costar) containing 1 mL of MammoCult basal medium (Stem Cell Technologies) supplied with 10% MammoCult proliferation supplement ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... the corrected and patient cell lines were maintained in plates coated with Geltrex (1:50 DMEM/F12) overnight using mTeSR (Stem Cell technologies). The media was refreshed daily for iPSCs ...
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bioRxiv - Cell Biology 2021Quote: ... 100ug were added to 50K GMPs sorted the day before and cultured in StemSpan SFEMII supplemented with 1% L-Glutamine and Penicillin/Streptomycin with no cytokines (Stem cell technologies). Cells were cultured in a humidified incubator at 37°C and 5% CO2 for 12 hours and then washed twice with PBS-2%FBS with 7AAD ...
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bioRxiv - Cancer Biology 2022Quote: 1×105 sorted CD34+ HSPCs were seeded in for collagen-based colony-forming assays (MegaCult™-C medium with cytokines, StemCell Technologies), as previously described (Gialesaki et al. ...
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
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bioRxiv - Cancer Biology 2020Quote: ... Each fragment was placed in 1 mL of freezing media (400 μL of NeuroCult NS-A Basal medium with proliferation supplement (StemCell Technologies; #05751) containing 20 μg/mL rhEGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... mTESR™ 1 medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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bioRxiv - Genetics 2021Quote: ... Cells were resuspended in 1 mL pre-warmed Neuronal Expansion Media with Rock inhibitor (Y-27632 compound, Stem Cell Technologies, cat# 72304) at a final concentration of 10 μM and a cell count performed ...
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 15 min at 37°C and adherent aNSCs/NPCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies, 07920) incubated for 5 min at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then resuspended at 1 million/mL in either Immunocult-XF T-cell Expansion Medium (Cat # 10981, STEMCELL Technologies, Vancouver, Canada) or TEXMACs Cell Culture Medium (Cat # 130-097-196 ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Developmental Biology 2023Quote: ... After 3 days of growth of naïve PSC were harvested and counted and 55 cells were seeded per microwell (total of 66,000 cells were seeded per 24-well in 1 ml of medium) AggreWell 400 24-well (Stemcell Technologies cat 34415) in N2B27-BSA supplemented with 10 µM ROCKi Y27632 ...
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bioRxiv - Cancer Biology 2023Quote: ... FACS-sorted cKit+Sca-1+Lin-cells isolated from murine bone marrow aspirates were cultured in methylcellulose median (Methocult GF#M3434, Stem Cell Technologies). The following independent experiments were performed in technical duplicates for each setting ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
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bioRxiv - Molecular Biology 2023Quote: ... and cultured in neuronal maintenance medium (MEM with L-glutamine, 0.6% glucose and 1× Neurocult SM1 neuronal supplement (STEMCELL Technologies, cat# 05711)) for 21 days replacing half of the medium every 3-4 days.
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bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
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bioRxiv - Cell Biology 2023Quote: ... Accutase was quenched and single cells were seeded for differentiation in suspension at a density of 1×106 cells/mL in complete mTeSR media (Stem Cell Technologies) supplemented with 10ng/mL FGF2 (Peprotech ...
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bioRxiv - Genomics 2023Quote: Female H9 hESCs were obtained from the WiCell Research Institute and cultured on Matrigel-coated culture dishes in mTeSR™1 media (Stemcell Technologies) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse bone marrow c-Kit+ cells from Rosa26-LSL-Cas9 knockin mice were transduced with the sgRNAs using the lentivirus and were selected for stable expression of the sgRNAs using puromycin (1 μg/ml) in MethoCult™ M3234 (STEMCELL Technologies) supplemented with 10 ng/mL mouse SCF ...
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bioRxiv - Cell Biology 2024Quote: ... HSPCs were thawed on day 0 into CD34+ expansion media consisting of Stem Span SFEMII supplemented with 1x StemSpan™ CD34+ expansion supplement and 1 μM of U729 (StemCell Technologies). On day 7 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were replated in mTeSR1 supplemented with 1% P/S and 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies, Vancouver, BC, Canada) for 24 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... engineered to contain a BxbI specific landing-pad expression cassette at the Pansio-1 safe harbour locus13 (Supp. File 7) were maintained using mTeSR medium (STEMCELL Technologies, 85850) on 1:200 Geltrex (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Positive cells were sorted into 96-well Matrigel-coated plate at 1 cell/well with 100 µl/well StemFlex and 1x CloneR2 (Stem Cell Technologies). 10-12 days later ...
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bioRxiv - Genomics 2024Quote: ... on Day -1 CD4+ T cells are isolated from blood (300 ml) of HIV seronegative donors by negative selection (StemCell Technologies, Inc). On Day 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Colony formation assays were established using primary AML BM cells cultured at 1×105 cells/ml in MethoCult-H4434 (Stem Cell Technologies) in the presence of vehicle or drug ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were then stained for F4/80+ positivity by first using a F4/80-PE conjugated antibody (StemCell Technologies Cat# 60027PE.1) and incubated for 15 min at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Bioengineering 2021Quote: ... Matrigel was used coat culture dishes for feeder-free expansion of induced pluripotent stem cells and were routinely cultured and maintained in mTeSR™1 (STEMCELL Technologies, 85850) in a humidified incubator at 37 °C with 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: H1 human ESCs were dissociated using accutase and plated as single cells in mTeSR™1 medium supplemented with ROCK inhibitor Y-27632 (10 μM, StemCell Technologies, Inc.) on matrigel to a final density of 20,000-30,000 cells per cm2 ...
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bioRxiv - Molecular Biology 2022Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using Lymphoprep™ and SepMate™ tubes (StemCell Technologies), and cryopreserved [41] ...
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bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were prepared by mechanically disaggregating the domes and digesting the Matrigel for 1 hour using dispase at 37°C (Stem Cell Technologies, 7913). Cells were washed in PBS and resuspended in lysis buffer as previously described (Santiago-Gomez et al ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in complete NSC medium (basal medium + proliferation supplement at a 9:1 ratio; NeuroCult™ NS-A Proliferation Kit, STEMCELL Technologies, 05751) supplemented with penicillin-streptomycin (1% final concentration ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was changed every second day and serial dilutions were performed in mTeSR+ medium containing Rock-inhibitor Y-27632 and CloneR (1:1000, Stem Cell Technologies #05889). Rock-inhibitor was removed after two days and the medium containing CloneR was changed every second day until colonies were ready to be picked ...
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Corning 10-092-CV; 20% fetal bovine serum [FBS], Sigma-Aldrich F4135; and 0.1 mg ml-1 DNase I, Stem Cell Technologies 07900). Cell suspension was placed in a new tube ...