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101 - 150 of 1054 citations for Recombinant Human IL17RA His tagged since 2019

• A human disease model of SARS-CoV-2-induced lung injury and immune responses with a microengineered organ chip

  Min Zhang, et al., bioRxiv - Bioengineering 2020

  Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
• LRRK2 and Rab10 Coordinate Macropinocytosis to Mediate Immunological Responses in Phagocytes

  Zhiyong Liu, et al., bioRxiv - Cell Biology 2020

  Quote: Human Monocyte were acquired from frozen PBMCs with STEMCELL EasySep Human Monocyte Enrichment kit (STEMCELL TECHNOLOGIES). Purified monocytes were cultured in RPMI-1640 Glutamax medium (Invitrogen ...
• Metabolic Deficiencies Underlie Plasmacytoid Dendritic Cell Exhaustion After Viral Infection

  Trever T. Greene, et al., bioRxiv - Immunology 2024

  Quote: Human pDC isolation was performed using an EasySep™ Human Plasmacytoid DC Enrichment kit (STEMCELL Technologies) following the manufacturer’s instructions.
• Functional Profiling of Single CRISPR/Cas9-Edited Human Long-Term Hematopoietic Stem Cells

  Elvin Wagenblast, et al., bioRxiv - Genetics 2019

  Quote: ... 10μl Human LDL (StemCell Technologies, 02698) for every 50ml media and the following cytokines (all from MiltenyiBiotec unless stated otherwise) ...
• Mapping the Cellular Origin and Early Evolution of Leukemia in Down Syndrome

  Elvin Wagenblast, et al., bioRxiv - Cancer Biology 2020

  Quote: ... 0.02% Human LDL (Stem Cell Technologies) and the following cytokines (Miltenyi Biotec unless stated otherwise) ...
• Targeting human Plasmacytoid dendritic cells through BDCA2 prevents inflammation and fibrosis in xenotransplant mouse model of Scleroderma

  Rebecca L. Ross, et al., bioRxiv - Immunology 2020

  Quote: ... commercial cryo-human pDC (Stemcell Technology) from 5 healthy subjects ...
• STAG3 promotes exit from pluripotency through post-transcriptional mRNA regulation in the cytoplasm

  Sam Weeks, et al., bioRxiv - Cell Biology 2024

  Quote: ... 0.1mM ßMercaptoethanol (BMe)) and freshly added mouse recombinant LIF (1000U/ml; Stemcell technologies 78056). 2i-cultured mESCswere grown on plates coated with Fibronectin (17.6μl/1ml PBS ...
• Characterization of prefusion-F-specific antibodies elicited by natural infection with human metapneumovirus

  Scott A. Rush, et al., bioRxiv - Immunology 2022

  Quote: B cells were enriched from human PBMCs using EasySep Human Pan-B Cell Enrichment Kit (StemCell Technologies) before being incubated with Fc block (BD Biosciences ...
• T Cell Receptor Immunotherapy Drives Human Immunodeficiency Virus Evolution in Humanized Mice

  Alok V. Joglekar, et al., bioRxiv - Immunology 2019

  Quote: ... Human CD45+ cells were enriched using the EasySep™ Mouse/Human Chimera Isolation Kit (Stemcell technologies, 19849) according to the manufacturer’s instructions ...
• Vaccination with SARS-CoV-2 Spike Protein and AS03 Adjuvant Induces Rapid Anamnestic Antibodies in the Lung and Protects Against Virus Challenge in Nonhuman Primates

  Joseph R. Francica, et al., bioRxiv - Immunology 2021

  Quote: ... Human NK cells were isolated the evening before using RosetteSep Human NK cell Enrichment cocktail (STEMCELL Technologies) from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15 ...
• Adjuvanting a subunit SARS-CoV-2 nanoparticle vaccine to induce protective immunity in non-human primates

  Prabhu S. Arunachalam, et al., bioRxiv - Immunology 2021

  Quote: ... Human NK cells were isolated the evening before using RosetteSep Human NK cell Enrichment cocktail (STEMCELL Technologies) from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (STEMCELL Technologies) ...
• High affinity chimeric antigen receptor signaling induces an inflammatory program in human regulatory T cells

  Russell W. Cochrane, et al., bioRxiv - Immunology 2024

  Quote: Human CD14+ monocytes were isolated from leukopaks using the EasySep Human CD14+ Positive Selection Kit (STEMCELL Technologies) and differentiated into monocyte-derived dendritic cells (moDCs ...
• Inosine Induces Stemness Features in CAR T cells and Enhances Potency

  Dorota D. Klysz, et al., bioRxiv - Immunology 2023

  Quote: ... Primary human T cells were isolated using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) according to the manufacturer’s protocol ...
• COVID-19 Severity Is Associated with Differential Antibody Fc-mediated Innate Immune Functions

  Opeyemi S. Adeniji, et al., bioRxiv - Microbiology 2021

  Quote: ... 1 × 105 human NK cells isolated from PBMC using EasySep human NK cell isolation kit (Stem Cell Technologies) were added to wells in the presence of CD107a PE (BD ...
• Realtime morphological characterization and sorting of unlabeled viable cells using deep learning

  Mahyar Salek, et al., bioRxiv - Cell Biology 2022

  Quote: ... human neutrophils were isolated from whole blood using the EasySep Direct Human Neutrophil Isolation kit from Stemcell Technologies by immunomagnetic negative selection ...
• Identification of Cross-Reactive CD8⁺ T Cell Receptors with High Functional Avidity to a SARS-CoV-2 Immunodominant Epitope and Its Natural Mutant Variants

  Chao Hu, et al., bioRxiv - Immunology 2020

  Quote: ... EasySep human CD4+ T cell enrichment kit and EasySep human CD8+ T cell enrichment kit (STEMCELL Technologies, Canada), respectively ...
• Discovery of prevalent, clinically actionable tumor neoepitopes via integrated biochemical and cell-based platforms

  Hem Gurung, et al., bioRxiv - Cancer Biology 2022

  Quote: CD8+ cells were enriched from human PBMCs with EasySep Human CD8+ T Cell Isolation Kit (Stem Cell Technologies) and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend ...
• Generation of natural killer and myeloid cells in a 3D artificial marrow organoid system

  Bérénice Schell, et al., bioRxiv - Cell Biology 2024

  Quote: ... Human CD34+ cells were magnetically enriched with the EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies) from either normal BM or CB samples ...
• Maturation, developmental site, and pathology dictate murine neutrophil function

  John B. G. Mackey, et al., bioRxiv - Immunology 2021

  Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
• Selective germline genome edited pigs and their long immune tolerance in Non Human Primates

  Lijin Zou, et al., bioRxiv - Synthetic Biology 2020

  Quote: Human natural killer cells (Stemcell Technologies, Canada) were incubated with 5×105 skin fibroblasts for 60 min at 37°C ...
• Modulation of Aplnr signaling is required during the development and maintenance of the hematopoietic system

  Melany Jackson, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and human (MethoCult™ H4434, Stemcell Technologies) differentiating PSC ...
• Identification of a Broadly Fibrogenic Macrophage Subset Induced by Type 3 Inflammation in Human and Murine Liver and Lung Fibrosis

  Thomas Fabre, et al., bioRxiv - Immunology 2022

  Quote: Cryopreserved primary human CD14+ monocytes (Stemcell Technologies) isolated from peripheral blood were plated at 300,000 cells/well in flat bottom ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... + 50 ng/ml human FGF2 (Stemcell Technologies) + 500 nM A-8301 (Selleckchem ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... + 50 ng/ml human FGF2 (Stemcell Technologies) + 500 nM A-83-01 + 1:500 Primocin + 10 µM Y-27632 (only for the first two days) ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... + 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 10 vol% conditioned medium containing Rspondin1 (produced from HA-R-Spondin1-Fc HEK293T cell line ...
• Reconstructing bat antiviral immunity using epithelial organoids

  Max J. Kellner, et al., bioRxiv - Immunology 2024

  Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 3 µM CHIR9902 (Stemcell Technologies ...
• A Novel Humanized Mouse Model for HIV and Tuberculosis Co-infection Studies

  José Alejandro Bohórquez, et al., bioRxiv - Immunology 2024

  Quote: ... frozen human PBMCs (STEMCELL Technologies, Vancouver, Canada) were thawed and seeded in a 75 cm2 flask at a concentration of 5 × 106 cells/mL in RPMI 1640 media (Corning Inc. ...
• Label-free ghost cytometry for manufacturing of cell therapy products

  Kazuki Teranishi, et al., bioRxiv - Cell Biology 2023

  Quote: Human PBMCs were purchased from STEMCELL Technologies and Precision for Medicine ...
• Adaptation to ex vivo culture drives human haematopoietic stem cell loss of repopulation capacity in a cell cycle independent manner

  Carys S. Johnson, et al., bioRxiv - Cell Biology 2023

  Quote: ... human LDL (50ng/ml) (Stem Cell Technologies) in addition to the following cytokines ...
• S100A8/A9 predicts triple-negative breast cancer response to PIM kinase and PD-1/PD-L1 inhibition

  Lauren R. Begg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD8+ T cells (StemCell Technologies, 70027), CD4+ T cells ...
• Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis

  Arek Kendirli, et al., bioRxiv - Immunology 2022

  Quote: ... CD4+ Human T cells were enriched from PBMCs using EasySep™ Human CD4+ T Cell Isolation Kit (StemCell Technologies) according to the manufacturers protocol ...
• Replication timing alterations in leukemia reflect stable clinically-relevant changes in genome architecture

  Juan Carlos Rivera-Mulia, et al., bioRxiv - Cancer Biology 2019

  Quote: ... A crude enrichment for human cells was performed using an EasySep™ Mouse/Human Chimera Isolation Kit (STEMCELL Technologies) as per manufacturer instructions but with only a single separation on the magnet and with an extra wash of the mouse cells retained on the magnet during the pour off ...
• Chemotaxis and swarming in differentiated HL60 neutrophil-like cells

  Kehinde Adebayo Babatunde, et al., bioRxiv - Cell Biology 2019

  Quote: ... Human neutrophils were isolated within 1h after drawn using the human neutrophil direct isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
• Potent neutralizing anti-SARS-CoV-2 human antibodies cure infection with SARS-CoV-2 variants in hamster model

  Maya Imbrechts, et al., bioRxiv - Immunology 2021

  Quote: Human B cells were enriched from cryopreserved PBMCs using the EasySep™ Human B Cell Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
• Epigenetic Remodeling by Vitamin C Potentiates the Differentiation of Mouse and Human Plasma Cells

  Heng-Yi Chen, et al., bioRxiv - Immunology 2021

  Quote: ... Primary human B cells were isolated from PBMCs using the EasySep Human Naïve B cell isolation kit (Stemcell Technologies). Naïve B cells were cultured with or without VC (100 µg/mL ...
• Septins Provide Microenvironment Sensing and Cortical Actomyosin Partitioning in Motile Amoeboid T Lymphocytes

  Alexander S. Zhovmer, et al., bioRxiv - Biophysics 2023

  Quote: Primary human CD4+ T cells were isolated from commercially available whole human blood (STEMCELL Technologies Inc., USA, Cat# 70507.1) with EasySep Human CD4+ T Cell Isolation Kits (STEMCELL Technologies Inc. ...
• Unleashing the Power of NR4A1 Degradation as a Novel Strategy for Cancer Immunotherapy

  Lei Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-human CD25-PE-Cy7 (clone MA251) plus FVD-eFluor-780 (eBioscience) and human FcR blocking Reagent (StemCell Technologies). Cells were washed then fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer Set ...
• Polycomb contraction differentially regulates terminal human hematopoietic differentiation programs

  A. Lorzadeh, et al., bioRxiv - Cell Biology 2020

  Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
• c-Jun Overexpressing CAR-T Cells are Exhaustion-Resistant and Mediate Enhanced Antitumor Activity

  Rachel C. Lynn, et al., bioRxiv - Immunology 2019

  Quote: Primary human T cells were isolated from healthy donors using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies). Buffy coats were purchased from Stanford Blood Center and processed according to the manufacturer’s protocol using Lymphoprep density gradient medium and SepMate-50 tubes ...
• Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression

  Katherine P. Mueller, et al., bioRxiv - Bioengineering 2021

  Quote: ... Primary human T cells were isolated using negative selection per the manufacturer’s instructions (RosetteSep Human T Cell Enrichment Cocktail, STEMCELL Technologies). T cells were counted using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific ...
• Blockade of redox second messengers inhibits JAK/STAT and MEK/ERK signaling sensitizing FLT3-mutant acute myeloid leukemia to targeted therapies

  Zacary P. Germon, et al., bioRxiv - Cancer Biology 2022

  Quote: Purified human neutrophils were extracted from whole blood using the EasySep™ Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, BC) and performed in accordance with the manufacturer’s protocol.
• Nuclear receptor corepressor 1 controls regulatory T cell subset differentiation and effector function

  Valentina Stolz, et al., bioRxiv - Immunology 2022

  Quote: ... Naive human CD4+ T cells were isolated using the EasySep Human Naïve CD4 TCell Iso Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
• miR-424(322) is a molecular switch controlling pro-inflammatory vs anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling

  Victoria Zyulina, et al., bioRxiv - Immunology 2020

  Quote: Human cord-blood CD34+ cells were isolated by magnetic sorting using EasySep human CD34 positive selection kit (Stem Cell Technologies) according to manufacturer’s protocol ...
• The potential role of human immune cells in the systemic dissemination of enterovirus-D68

  Brigitta M. Laksono, et al., bioRxiv - Microbiology 2022

  Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
• Engineered CD47 protects T cells for enhanced antitumor immunity

  Sean A. Yamada-Hunter, et al., bioRxiv - Bioengineering 2023

  Quote: ... Primary human T cells were purified by negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes ...
• Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host disease

  Kyle T. Reid, et al., bioRxiv - Immunology 2023

  Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
• Single-Cell Analysis of 5-ALA Intraoperative Labeling Specificity for Glioblastoma

  Zhouzerui Liu, et al., bioRxiv - Cancer Biology 2022

  Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... total PBMCs or purified human naïve CD4+ T cells were stimulated with the ImmunoCult human T cell activator (StemCell Technologies) in the presence of either DMSO or soquelitinib for 72 hours ...