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Citations for Stemcell Technologies :
101 - 150 of 1131 citations for Recombinant Human FCGRT & B2M Protein His Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... CD34+ cells were enriched with the EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) without using the RosetteSep™ Human Cord Blood CD34 Pre-Enrichment Cocktail ...
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bioRxiv - Cancer Biology 2022Quote: ... CD20+ target cells were isolated using the EasySep Human Biotin Positive Selection Kit II (StemCell Technologies, Canada) and the biotinylated anti-CD20 antibody (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655, STEMCELL Technologies) containing 1% penicillin– streptomycin together with 10 ng/ml rhSCF (#255-SC ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Developmental Biology 2022Quote: ... 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies, 18356) to positively select for epithelial cells ...
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bioRxiv - Molecular Biology 2022Quote: ... CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies, Cat 17856) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... positive immunomagnetic selection of CD45+ cells were performed with EasySep Human CD45 Depletion Kit II (StemCell Technologies, Canada) according to the manufacturer instructions (post-isolation purity 96-99%) ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ cells were isolated from the purified PBMNCs by EasySep Human CD34 positive selection kit II (STEMCELL Technologies) and expanded in HSC expansion media as described earlier (Genovese et al ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by CD34 magnetic bead-based enrichment using the EasySep human CD34 positive selection kit II (StemCell technologies). We found this enrichment strategy to be simple and reproducible and chose it for several reasons ...
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bioRxiv - Immunology 2023Quote: ... and CD14+ cells were purified using the EasySep™ Human CD14 Positive Selection Kit II (StemCell Technologies, 17858). A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... CD34+ cells were isolated using EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, BC, Canada) as per manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II for future studies (Stemcell Technologies, #17877). The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit ...
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bioRxiv - Cell Biology 2020Quote: Cord blood (CB) CD34+ cells were purified with Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies, 17896) and cultured in SFEM II medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T cells were isolated from monocyte-depleted PBMCs using EasySepTM Human CD4+ Selection Kit II (StemCell Technologies Ltd.) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... 1% Pen/Strep) plus ALI (1μM A83-01 (Stem cell Technologies), 100nM LDN-193189(Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were isolated from umbilical cord blood using the EasySep™ Human CD34 Positive Selection Kit II (Stemcell Technologies). Cytapheresis of CML patients were kindly provided by Pr ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues at 19 w and 20 w were subjected to EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies, 18259) to remove CD45+ immune cells ...
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bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were isolated using a EasySep Human CD34 Positive Selection Kit II according to the manufacturer’s protocol (STEMCELL Technologies). CD34+ HSPCs were cultured at 1×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: HSPC (CD34+) were isolated from cord blood using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies) according to the manufacture’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
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bioRxiv - Bioengineering 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights™ EasySep™ Magnets (StemCell Technologies) according to the manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
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bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... density-gradient centrifugation and CD34+ cells were isolated using anti-CD34 immunomagnetic beads (positive selection) following the manufacturer’s instructions (EasySepTM Human cord blood CD34+ positive selection kit II, STEMCELL Technologies Cat#17896). CD34+ HSPCs were either freshly used or cryo-preserved until future use ...
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bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2022Quote: ... and 30 μl of additional biotinylated anti-CD3 (STEMCELL Technologies, 18051) was added to the sample to improve purity ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Cancer Biology 2022Quote: ... or StemSpan Serum Free Expansion Media II (SFEM II) (Stem Cell Technologies), supplemented with human stem cell factor (SCF ...
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bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng/mL recombinant murine SCF (StemCell Technologies cat. # 78064), and 1 mg/mL polyvinyl alcohol (Sigma cat ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng/mL recombinant IL-6 (StemCell Technologies cat. #78052.1) 3 ng/mL recombinant murine IL-1α (PeproTech ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... 40% SFEM II (StemCell Technologies) and 10% DMSO (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... Human NK cell or Human Monocyte CD14+ Isolation kits (STEMCELL Technologies), respectively ...
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bioRxiv - Bioengineering 2023Quote: ... + 5% fetal bovine serum + 1% Pen/Strep (Mediatech, MT30-002-CI) +/- 30 µM Y27632 (StemCell Technologies, 72304) was added to just cover the dissected kidneys ...
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bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant Fms-like tyrosine kinase-3 (Flt3, StemCell Technologies Cat#78009), Erythropoeitin (EPO ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were cultured in StemSpan™ Serum-Free Expansion Medium version II (SFEM II; STEMCELL Technologies) containing 50 ng/mL each of recombinant human stem cell factor (SCF ...
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bioRxiv - Immunology 2024Quote: ... + 1:100 Glutamax + 10 mM HEPES + 1 mM N-AcetylCystein + 1:50 B-27 Supplement + 50 ng/ml human EGF + 100 ng/ml human Noggin +100 ng/ml human IGF1 (Stemcell Technologies) + 50 ng/ml human FGF2 (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Selection kit II (STEMCELL Technologies, #17877) according to the manufacturer’s instructions ...