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Citations for Stemcell Technologies :
101 - 150 of 749 citations for Mouse Anti Hepatitis C Virus NS4a b Antibody 1865 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Antibodies and fluorochromes used: Mouse hematopoietic progenitor cell isolation cocktail (Stem Cell Technologies 19856), CD48-APC (Thermo-Fischer 17-0481-82) ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then enriched for B cells by using the EasySep Human Pan-B cell Enrichment Kit (Stemcell Technologies, Vancouver, BC, Canada). PBMCs were incubated with an antibody cocktail containing CD2 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2019Quote: ... The sample was resuspended in growth media and B cells were negatively selected using the EasySep Human B cell Isolation Kit according to the manufacturer’s protocol (STEMCELL Technologies, Vancouver, BC). Because our samples contained greater than one million PBMCs ...
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bioRxiv - Immunology 2024Quote: ... 1.5 µg/mL anti-human CD32 antibody (STEMCELL Technologies) and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed ...
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bioRxiv - Microbiology 2019Quote: ... or RosetteSep Human B cell enrichment cocktail (STEMCELL Technologies) and then isolated with Ficoll-Paque Plus (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary cells were resuspended in Epicult-B (Stemcell Technologies) media containing 2% Matrigel and 12,000 cells were seeded into each chamber in a total volume of 400 μL ...
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bioRxiv - Cancer Biology 2021Quote: ... and immersed in EpiCult-B Medium (STEMCELL Technologies, 05610) supplemented with 10 ng/mL human recombinant EGF (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated by negative magnetic selection from either B*08:01+ or B*35:01+ donor blood using kits from StemCell Technologies (catalog #17953). To screen for antigen-specific CTLs ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... T cells were activated with anti-CD3/anti-CD28 tetrameric antibody complexes (Stemcell Technologies, #10971) on days 1 and 13 of culture and expanded every 3-4 days ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were replated in prepared Medium B from Stemcell technologies. The manufacturer’s protocol was followed for an additional 7-10 days and numerous CD43+ progenitor cells (HPC ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Microbiology 2020Quote: ... Primary human B cells were isolated by negative selection using RosetteSep Human B Cell Enrichment and EasySep Human B cell enrichment kits (Stem Cell Technologies, #15064 and #19054), according to the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
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bioRxiv - Physiology 2020Quote: ... enteroids were maintained in Human IntestiCult components A+B (STEMCELL Technologies). To promote differentiation ...
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bioRxiv - Microbiology 2024Quote: ... the Easy Human Pan-B Cell Enrichment kit (STEMCELL Technologies #19554) was used ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...
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bioRxiv - Neuroscience 2019Quote: ... anti-human CD40 antibody (clone 5C3) and 20 ng rhIL4 (Stemcell Technologies) was added to culture medium ...
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bioRxiv - Immunology 2021Quote: ... after B cell enrichment with the negative selection cocktail (Stem Cell Technologies), cells were labeled with anti-CD19-PEcy7 ...
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bioRxiv - Immunology 2022Quote: ... The blood was treated with 2.5 μl/ml Rosettesep B (Stemcell technologies) for 20 mins at room temperature.
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bioRxiv - Immunology 2020Quote: ... using the EasySep Human Naive B Cell Enrichment Kit (19254; STEMCELL Technologies), from healthy donor PBMCs ...
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bioRxiv - Immunology 2023Quote: ... TMNCs or purified B cells were frozen in Cryostor (Stem Cell Technologies) and stored in liquid nitrogen until use.
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bioRxiv - Immunology 2023Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies and thawed per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were needle triturated and resuspended in Mouse NSC Proliferation Media (1x Anti-Anti, 10ng/mL bFGF (#78003, Stem Cell Tech, Vancouver), 20ng/mL mEGF (#78016 ...
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bioRxiv - Biophysics 2020Quote: ... of either sex using a B-cell isolation kit (Stemcell Technologies; catalogue #19854) to deplete non-B cells ...
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bioRxiv - Neuroscience 2021Quote: ... medium was replaced with 2 mL/well iHSC Medium B (StemCell Technologies #05310). An additional 1 mL/well of iHSC Medium B was added every other day ...
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bioRxiv - Cancer Biology 2022Quote: ... the whole BM samples were incubated with Human B RosetteSep (Stemcell Technologies, CAN) following the manufacturer ‘instructions.
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bioRxiv - Cancer Biology 2019Quote: ... in complete Epicult-B Medium supplemented with 0.48 µg/mL hydrocortisone (Stemcell technologies) overnight at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... and naïve B cells were isolated by negative selection (StemCell Technology, Catalog #19254). B cells were seeded in 24 well flat-bottom plates at a density of 1 × 105 per well and stimulated in 1 ml of B cells complete medium ...
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bioRxiv - Immunology 2022Quote: ... Splenic B cells were enriched with the EasySep kit (STEMCELL Technologies, Cambridge, MA). Bone marrow cells were obtained by flushing femora and tibiae with RPMI medium ...
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bioRxiv - Immunology 2022Quote: ... for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 7 days as described previously (41) ...
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bioRxiv - Microbiology 2020Quote: ... Anti-CD3/CD28 antibody-coated beads (ImmunoCult) were purchased from STEMCELL technologies (Vancouver, CA).
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies (Vancouver, Canada). Cells were thawed per manufacturer’s instructions and cultured as previously described.60 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.25 μg/mL amphotericin B) supplemented with 10 μM Y-27632 (STEMCELL Technologies). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... anti-CCR10 antibodies and DAPI together with control peripheral blood mononuclear cells (PBMC; Stemcell Technologies). Mature B cells were gated from single cells ...
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bioRxiv - Cancer Biology 2020Quote: Myeloma cells were purified using nanobeads and an anti-CD138 antibody (RoboSep, Stem Cell Technologies). After immunomagnetic sorting ...
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bioRxiv - Biochemistry 2023Quote: ... primary CD4+ T cells were activated with anti-CD3/CD28 antibodies (cat#10971, Stemcell Technologies). 1e6 activated primary CD4+ T cells were electroporated with 1μg PEmax variant mRNA ...
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bioRxiv - Immunology 2021Quote: ... Isolation of T and B cells were performed according to the manufacturer’s protocols (Stemcell Technologies).
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bioRxiv - Pathology 2019Quote: ... iPSCs were exposed to N2 media containing 5 mL N2 Supplement-B (Stemcell Technologies 07156), 0.5mL 55mM β-mercaptoethanol (Life technologies 21985-023) ...
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with RPMI1640 enriched with 5ng/mL PDGF-B (Stem Cell Technologies), 2.5 ng/mL TGF-B (PeproTech) ...