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Citations for Stemcell Technologies :
101 - 150 of 241 citations for 7 Diethylamino 3 thenoyl coumarin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and 3 µM of CHIR99021 (GSK3B inhibitor, STEMCELL Technologies, cat # 72054). From day 1 to 3 ENR was supplemented with 20% Wnt3a-CM and 10 μM Y-27632.
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bioRxiv - Neuroscience 2022Quote: ... 3×106 single-cell suspension (Gentle Cell Dissociation Reagent, StemCell Technologies) of iPSCs were plated ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
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bioRxiv - Developmental Biology 2024Quote: ... in motor neuron differentiation medium with 3 uM CHIR99021 (Stemcell Technologies) and cultured for 2 days to form neurospheres ...
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bioRxiv - Genomics 2024Quote: ... 3 x 106 cells were dissociated with Accutase (Stemcell Tech, 7920) and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Immunology 2024Quote: ... Macrophages were differentiated from the expanded CD34+ cells by first culturing for 7 days in StemSpan SFEM II supplemented with StemSpan Myeloid Expansion Supplement II (StemCell Tech 02694), and then culturing for another 7 days in IMDM media containing 20 ng/mL MCSF (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Genetics 2023Quote: ... Lentivirus media was removed after 24 hours and the cells were allowed to rest for another 24 hours before being selected for 7 days with Puromycin at 8µg/ml (73342, STEMCELL Technologies, Cambridge, MA, USA). Surviving cells were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 3–4 days using Accutase (Stemcell Technologies). The iPS cells conversion to EPS as previous reported47.
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Cell Biology 2020Quote: ... 3 × 106 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form EBs ...
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bioRxiv - Cell Biology 2024Quote: ... Passaging was performed every 3-4 days using ReLeSR (Stemcell Technologies, #05872) according to manufacturer’s instructions ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were passaged at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured in NPC medium on Matrigel coated plates onwards.
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bioRxiv - Developmental Biology 2021Quote: ... 5% CO2 for 14 days and the CFC content was automatedly imaged after 7 and 14 days by using the StemVision imaging system (Stem Cell Technologies, Version 2.0.1.0). Additionally ...
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Molecular Biology 2021Quote: ... 3 days post-transfection in 1 ml STEMdiff Neural Progenitor Medium (Stemcell Technologies) cells were washed by DPBS and 2 wells of the plate were put together and collected by centrifugation at 10.000 rpm for 2 min generating 3 biological replicates ...
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bioRxiv - Bioengineering 2021Quote: ... single cells suspended in a 3:4 mixture of Mammocult (StemCell Technologies 05620) and Matrigel (Corning 354234 ...
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bioRxiv - Microbiology 2022Quote: ... and heat shock (3 hours at 43 □C) in BrainPhys (Stem Cell Technologies) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Neuroscience 2020Quote: ... This medium was supplemented with 3 μM CHIR99021 (STEMCELL Technologies; Cat. No. 72054), 2 μM DMH1 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... cells were expanded for 3 days in StemSpan medium (Stem cell Technologies Inc) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 cells were then plated onto microwells (AggreWell 800, STEMCELL technologies) for embryoid body induction ...
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bioRxiv - Immunology 2022Quote: ... for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 7 days as described previously (41) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Systems Biology 2023Quote: ... we passaged the cells at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured cells in NPCs medium onwards.
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bioRxiv - Systems Biology 2023Quote: ... Cells were passaged 1:10 every 3 days using ReLeSR (StemCell Technologies #05873). Cells were maintained in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Bioengineering 2019Quote: ... SVF was plated at a cell density of 7×106 in T75 tissue culture flasks in 15 ml of MesenCult MSC basal medium (Stem Cell Technologies, Cambridge, MA, USA) supplemented with MesenCult MSC Stimulatory Supplement ...
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bioRxiv - Neuroscience 2020Quote: ... cells at week 3 of terminal differentiation were switched to BrainPhys medium (STEMCELL Technologies), and experiments were conducted after a total of 5–6 weeks of differentiation ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented for 3 days with 10 μM Rock inhibitor Y-27632 (StemCell Technologies, 78003).
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bioRxiv - Genetics 2021Quote: ... iPSC cultures were passaged every 3-4 days with Accutase (Stem Cell Technologies, 07920) or ReLeSR (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in the presence of 3 μM CHIR-99021 (Stem Cell Technologies). Then cells were cultured for 1 day without cytokines ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923) for 12-15 minutes at 37°C ...
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bioRxiv - Genetics 2019Quote: ... Cells were rinsed with PBS (5 mL) and treated with 3 mL Accutase (STEMCELL technologies) for 5 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... FcγR-specific mAbs were obtained from Stem Cell technologies (CD16: clone 3G8; CD32: IV.3). Reverse sICs were generated from these receptor-specific antibodies using goat-anti-mouse IgG F(ab)2 fragments (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Plates were pre-coated with coated with 3 μg/ml of vitronectin (07180, Stemcell Technologies) and 5mg/mL Col IV (C7521-5MG ...
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bioRxiv - Immunology 2021Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 × 106 cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8™ Flex Media supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 3 million cells were transferred to each well in AggreWell 800 (STEMCELL Technologies, 34815) and centrifuged at 100g for 3 minutes in hiPSC medium supplemented with ROCK inhibitor Y-27632 (EMD Chemicals) ...
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bioRxiv - Bioengineering 2023Quote: ... these cells were used in 3 mL of methylcellulose medium (H4435; Stem Cell Technology, Canada) plated in a 35-mm culture dish (Grainger) ...
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bioRxiv - Cancer Biology 2023Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...