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Citations for Stemcell Technologies :
101 - 150 of 804 citations for 7 Bromo 6 chloro 3 3 3 hydroxy 2 piperidyl 2 oxopropyl quinazolin 4 3H one monohydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and 3 µM of CHIR99021 (GSK3B inhibitor, STEMCELL Technologies, cat # 72054). From day 1 to 3 ENR was supplemented with 20% Wnt3a-CM and 10 μM Y-27632.
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bioRxiv - Neuroscience 2022Quote: ... 3×106 single-cell suspension (Gentle Cell Dissociation Reagent, StemCell Technologies) of iPSCs were plated ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
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bioRxiv - Developmental Biology 2024Quote: ... in motor neuron differentiation medium with 3 uM CHIR99021 (Stemcell Technologies) and cultured for 2 days to form neurospheres ...
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bioRxiv - Genomics 2024Quote: ... 3 x 106 cells were dissociated with Accutase (Stemcell Tech, 7920) and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza ...
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bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Cell Biology 2020Quote: ... 3 × 106 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form EBs ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were passaged at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured in NPC medium on Matrigel coated plates onwards.
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 days post-transfection in 1 ml STEMdiff Neural Progenitor Medium (Stemcell Technologies) cells were washed by DPBS and 2 wells of the plate were put together and collected by centrifugation at 10.000 rpm for 2 min generating 3 biological replicates ...
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bioRxiv - Microbiology 2022Quote: ... and heat shock (3 hours at 43 □C) in BrainPhys (Stem Cell Technologies) supplemented with 2 mM L-Glutamine ...
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bioRxiv - Neuroscience 2020Quote: ... This medium was supplemented with 3 μM CHIR99021 (STEMCELL Technologies; Cat. No. 72054), 2 μM DMH1 (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... cells were expanded for 3 days in StemSpan medium (Stem cell Technologies Inc) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 cells were then plated onto microwells (AggreWell 800, STEMCELL technologies) for embryoid body induction ...
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bioRxiv - Immunology 2022Quote: ... for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 7 days as described previously (41) ...
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bioRxiv - Systems Biology 2023Quote: ... we passaged the cells at 1:3 using Accutase (STEMCELL Technologies, Cat#07920) and cultured cells in NPCs medium onwards.
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bioRxiv - Systems Biology 2023Quote: ... Cells were passaged 1:10 every 3 days using ReLeSR (StemCell Technologies #05873). Cells were maintained in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Neuroscience 2020Quote: ... hiPSC colonies were passaged 1:6 every 4 to 7 days with Gentle Cell Dissociation Reagent (STEMCELL Technologies). Genomic integrity was validated by G-band karyotyping (Cell Line Genetics ...
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bioRxiv - Neuroscience 2020Quote: ... cells at week 3 of terminal differentiation were switched to BrainPhys medium (STEMCELL Technologies), and experiments were conducted after a total of 5–6 weeks of differentiation ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented for 3 days with 10 μM Rock inhibitor Y-27632 (StemCell Technologies, 78003).
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in the presence of 3 μM CHIR-99021 (Stem Cell Technologies). Then cells were cultured for 1 day without cytokines ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were maintained with media changes every 3-4 days with full neural culture media consisting of BrainPhys™ Basal Media (STEMCELL Technologies, Vancouver, BC, Canada) or Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923) for 12-15 minutes at 37°C ...
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bioRxiv - Genetics 2019Quote: ... Cells were rinsed with PBS (5 mL) and treated with 3 mL Accutase (STEMCELL technologies) for 5 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... FcγR-specific mAbs were obtained from Stem Cell technologies (CD16: clone 3G8; CD32: IV.3). Reverse sICs were generated from these receptor-specific antibodies using goat-anti-mouse IgG F(ab)2 fragments (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Plates were pre-coated with coated with 3 μg/ml of vitronectin (07180, Stemcell Technologies) and 5mg/mL Col IV (C7521-5MG ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 × 106 cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8™ Flex Media supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 3 million cells were transferred to each well in AggreWell 800 (STEMCELL Technologies, 34815) and centrifuged at 100g for 3 minutes in hiPSC medium supplemented with ROCK inhibitor Y-27632 (EMD Chemicals) ...
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bioRxiv - Bioengineering 2023Quote: ... these cells were used in 3 mL of methylcellulose medium (H4435; Stem Cell Technology, Canada) plated in a 35-mm culture dish (Grainger) ...
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bioRxiv - Immunology 2023Quote: ... mice were given a daily 3 µmol/kg intraperitoneal injection of dibenzazepine (DBZ) (StemCell Technologies) which was first dissolved as a 10 µmol/ml in DMSO stock solution (kept frozen at -20C ...
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bioRxiv - Microbiology 2020Quote: ... the induced hAFECs was cultured with the SFM medium supplemented with 3 μM CHIR99021 (STEMCELL Technologies), 10 ng/ml BMP4 (Peprotech) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... complete solution was removed and 3 ml 2i+Lif medium + 10uM ROCK inhibitor (Stemcell Technologies, 72304) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5-7 days and embedded into 2 mg/ml collagen type-I (StemCell Technologies, Vancouver, Canada) gels ...
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bioRxiv - Cell Biology 2020Quote: ... then 300 µL of this solution was added in 3 mL of MethoCult TM GF3434 (STEMCELL Technologies) to obtain a final concentration of 104 cells ...
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bioRxiv - Cell Biology 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... and supplemented with 20 ng/ml BDNF and 20 ng/ml Neurotrophin-3 (NT3, #78074; STEMCELL Technologies) until day 43 ...
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bioRxiv - Neuroscience 2021Quote: ... approximately 3 × 106 single cells were added per well in the AggreWell 800 plate (STEMCELL Technologies, 34815) with Essential 8 flex medium supplemented with Stemgent hES Cell Cloning & Recovery Supplement (1X ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred at 3 x 106 cells to a well of an AgrreWell800 plate (Stem Cell Technologies) in STEMdiff Neural Induction Medium + SMADi (Stem Cell Technologies ...
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Single cell determination of cardiac microtissue structure and function using light sheet microscopybioRxiv - Bioengineering 2020Quote: ... plates at a concentration of 3×104 cells/cm2 in mTeSR1 medium (Stem Cell Technologies, Vancouver, CA) supplemented with 10µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Microbiology 2020Quote: CD34+ HPCs from 3 independent donors were thawed and plated in StemSpan SFEM II media (Stemcell Technologies) containing 10% BIT serum substitute ...
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bioRxiv - Immunology 2022Quote: ... approximately 3×104 total Ai14 BM cells were plated per well in MethoCult (M3434, Stem Cell Technologies) enriched with rmSCF ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs are cultured and passaged for 3 times in STEMdiff™ Neural Induction Medium + SMADi (Stemcell Technologies) for up to 3 weeks ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.