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Citations for Stemcell Technologies :
1401 - 1424 of 1424 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were then stained for F4/80+ positivity by first using a F4/80-PE conjugated antibody (StemCell Technologies Cat# 60027PE.1) and incubated for 15 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: Testicular organoids were made by mixing germ and somatic cells at a 1:4 ratio and placing them into AggreWell800™ microwells (STEMCELL Technologies, 34811) overnight ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Bioengineering 2021Quote: ... Matrigel was used coat culture dishes for feeder-free expansion of induced pluripotent stem cells and were routinely cultured and maintained in mTeSR™1 (STEMCELL Technologies, 85850) in a humidified incubator at 37 °C with 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: H1 human ESCs were dissociated using accutase and plated as single cells in mTeSR™1 medium supplemented with ROCK inhibitor Y-27632 (10 μM, StemCell Technologies, Inc.) on matrigel to a final density of 20,000-30,000 cells per cm2 ...
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bioRxiv - Molecular Biology 2022Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using Lymphoprep™ and SepMate™ tubes (StemCell Technologies), and cryopreserved [41] ...
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bioRxiv - Bioengineering 2021Quote: ... enriched lineage negative cells were further purified to obtain an enriched Lin-Sca-1+ c-kit+ (LSK) population using EasySep™ Mouse Sca1 and cKit Positive Selection kits (#18756 & #18757, Stemcell Technologies, CA). For ROS studies ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were prepared by mechanically disaggregating the domes and digesting the Matrigel for 1 hour using dispase at 37°C (Stem Cell Technologies, 7913). Cells were washed in PBS and resuspended in lysis buffer as previously described (Santiago-Gomez et al ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in complete NSC medium (basal medium + proliferation supplement at a 9:1 ratio; NeuroCult™ NS-A Proliferation Kit, STEMCELL Technologies, 05751) supplemented with penicillin-streptomycin (1% final concentration ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was changed every second day and serial dilutions were performed in mTeSR+ medium containing Rock-inhibitor Y-27632 and CloneR (1:1000, Stem Cell Technologies #05889). Rock-inhibitor was removed after two days and the medium containing CloneR was changed every second day until colonies were ready to be picked ...
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ, Stem Cell Technologies). One well of seeded cells was left untreated as negative control (data not shown) ...
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bioRxiv - Genetics 2023Quote: ... we added the following procedures throughout this protocol: (1) we pretreated all plasticware that came into contact with cell aggregates with an anti-adhesion solution (Stem Cell Technology #07010) per manufacturer instructions and (2 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... and then re-seeded in supplemented mTeSR1 medium with 1% penicillin–streptomycin and a 10 μM concentration of the ROCK inhibitor Y-27632 (Stem Cell Technologies, 72308). Further details for cell culture reagents and consumables can be found in the Resource Table and standard protocols can be found at www.allencell.org.
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bioRxiv - Developmental Biology 2021Quote: E14 and its derivative reporter lines pMsgn1-Venus [1] and Hes7-Achilles [2] mouse ESCs were maintained under feeder-free conditions in gelatin-coated dishes (StemCell Technologies cat. no. 07903) with 2i medium composed of high glucose DMEM (Gibco cat ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Cell Biology 2023Quote: ... and the picked colonies were allowed to grow larger and they ‘passaged’ by mechanical splitting into a new Matrigel coated plate containing mTeSR™1 media with its supplement(Anonymous, n.d.) and maintained as suggested by the manufacturer (StemCell Technologies, Canada Cat. No. #85850). Subsequent passages were done by enzymatic dissociation (Accutase® ...
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bioRxiv - Cell Biology 2023Quote: Transduced lineage negative (Lin-) or Lin-Sca-1+Kit+ (LSK) cells were plated into 1mL of methylcellulose-based medium with cytokines (Stem Cell Technology, MethoCult GF M3434) into 35 x 10 mm non-adherent Bio-One Petri Dish ...
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bioRxiv - Cell Biology 2022Quote: WIBR3 (NIHhESC-10-0079) hESCs and isogenic LIS1+/- 10 were grown and maintained on irradiated MEFs in optimal naive NHSM conditions (RSET-Stem Cell Technologies INC, supplementary Table 1) 90 ...