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Citations for Stemcell Technologies :
1401 - 1450 of 1483 citations for Mouse Junction Plakoglobin JUP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Human primary total T cells (CD4 and CD8) were isolated from normal healthy donors following leukapheresis by negative selection using RosetteSep kits (Stemcell Technologies, Vancouver, CA). All specimens were collected with protocol approved by University Review Board and written informed consent was obtained from each donor ...
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bioRxiv - Bioengineering 2021Quote: ... and CD34+ cells were isolated using EasySep™ CD34 positive selection kit II (samples from Basel, Cat# 17896, Stemcell Technologies, Vancouver, BC, Canada).
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Immunology 2022Quote: Preparation of cells: Human CD4+ cells were isolated from buffy coats using EasySep™ Human CD4+ T Cell Isolation Kit (Stemcell Tech, # 17952), resuspended with complete RPMI (cRPMI ...
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bioRxiv - Neuroscience 2021Quote: ... 24 hours before each recording media was changed to supplemented BrainPhys™ Neuronal Medium and SM1 Kit (STEMCELL Technologies SARL, cat no. #05792). Axion Integrated Studio (AxIS 2.1 ...
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bioRxiv - Neuroscience 2021Quote: ... following a monolayer culture method with a commercial dual SMAD inhibition-mediated neural induction medium (STEMdiff™ SMADi Neural Induction Kit, Stem Cell Technologies) based on a published protocol59 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated by negative magnetic selection from either B*08:01+ or B*35:01+ donor blood using kits from StemCell Technologies (catalog #17953). To screen for antigen-specific CTLs ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Evolutionary Biology 2021Quote: Diploid and tetraploid iPSC lines were differentiated with the STEMdiff™ Trilineage Differentiation Kit according to the manufacturer’s instructions (STEMCELL Technologies, cat #05230). Differentiation was assessed using reverse transcription quantitative PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2021Quote: Spheroid cultures from patient-derived xenograft models were prepared by homogenizing the freshly isolated tumors from mice brains by pipetting in NeuroCult™ NS-A Proliferation Kit (STEMCELL Technologies, 05751). Homogenized tissue is then filtered to get single cell suspension ...
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bioRxiv - Immunology 2020Quote: ... we performed negative B cell enrichment from 100M PBMCs in each experiment using a commercially available B cell enrichment kit according to manufacturer’s protocol (StemCell Technologies; Vancouver, BC, Canada).
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bioRxiv - Immunology 2021Quote: Purified CD4+CD25− T cells were magnetically isolated from spleen and peripheral lymph node mononuclear cells using an EasySep magnetic T cell negative isolation kit (Stem Cell Technologies, Inc.) with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL ...
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bioRxiv - Immunology 2021Quote: Assays were performed on purified human classical monocytes isolated using immunomagnetic negative sorting (EasySep Direct Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, MA). As we have previously described 88 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mice were anesthetized with a solution consisting of 200 mg/kg ketamine and 20 mg/kg xylazine and perfused with PBS before neoplastic tissue was processed with the NeuroCult™ enzymatic dissociation Kit (StemCell Technologies, 05715) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in complete NSC medium (basal medium + proliferation supplement at a 9:1 ratio; NeuroCult™ NS-A Proliferation Kit, STEMCELL Technologies, 05751) supplemented with penicillin-streptomycin (1% final concentration ...
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bioRxiv - Developmental Biology 2022Quote: ... by plating them at 125.000 cells/cm2 on Matrigel and growing the cells in STEMdiff™ Neuron Differentiation Kit (Stem Cell Technologies, #08500) medium for one week with daily medium changes.
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bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Immunology 2022Quote: ... Splenic T cells were enriched using a magnetic-based commercial CD8 T cell negative selection kit (StemCell Technologies, BC, Canada; Supplemental Figure 3A) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Total CD4+ T-cells were obtained by negative selection from the spleen of wild-type mice using EasySepTM magnetic separation per kit instructions (product number 19851, STEMCELL Technologies, Vancouver, Canada). Purified total CD4+ were enumerated and 5×105 total CD4+ T-cells were injected via tail vein to recipient mice ...
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bioRxiv - Bioengineering 2023Quote: ... CD31+ EPCs were purified using a FITC-conjugated human CD31 antibody (Miltenyi, cat# 130-117-390, clone AC128) and the EasySep Human FITC Positive Selection Kit II (Stemcell Technologies, cat# 18558). Purified EPCs were seeded into collagen IV (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Cell Biology 2023Quote: ... The middle layer containing PBMCs were then enriched for primary human NK cells (hNK) by negative selection using the EasySep Human NK Cell Enrichment Kit (StemCell Technologies, Cat# 19055), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... After 24 hours cells were washed with D-PBS before media was exchanged for astrocyte differentiation medium (STEMdifftm Astrocyte differentiation kit #100-0013, StemCell Technologies, Cambridge, UK) and a full media exchange was completed every day for 7 days ...
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bioRxiv - Developmental Biology 2023Quote: PP cells generated using the STEMdiff™ Pancreatic Progenitor Kit and expanded under CINI conditions were dissociated into single cells using AccutaseTM (STEMCELL Technologies, 07920) at 37 °C and then resuspended in PEP medium (Table S4 ...
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bioRxiv - Neuroscience 2023Quote: ... A crude live cell mixture was obtained by filtration and negative selection using Easy Eights EasySep Magnet and Dead Cell Removal kit (Stemcell Technologies, Catalog # 17899). Cells were further en-riched with positive CD90.2 selection (Stemcell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 24-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276) and 1x Penn/Strep from a 100x stock (Mediatech ...
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bioRxiv - Biochemistry 2024Quote: Human CD4+CCR6+CXCR3- T cells were isolated from human donor leukopaks using EasySep™ Human Th17 Cell Enrichment Kit (StemCell Technologies, 18162). To obtain large quantities of cells ...
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bioRxiv - Bioengineering 2024Quote: The primary T cells were obtained from peripheral venous blood of a healthy human donor using the EasySep™ Direct Human T cell Isolation Kit (STEMCELL TECHNOLOGIES, #19661) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... hiPSCs from N=1 donor were differentiated to definitive endoderm by using the STEMdiff™ Definitive Endoderm Kit (STEMCELL Tech., Vancouver, BC, Canada). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were isolated from frozen PBMC tubes using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies, #17952) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... neutrophils were isolated from human whole blood based on immuno-magnetic negative isolation technique using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Cat# 19666) described in detail in (Fan et al ...
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bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: We isolated CD34+ cells from human cord blood and BM samples using an EasySep™ Human CD34 Positive Selection Kit II (STEMCELL Technologies, Vancouver, Canada); sample purity of >95% was confirmed using flow cytometry ...
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bioRxiv - Microbiology 2019Quote: ... Isolated PBMCs were used to isolate CD4+ T-cells by negative selection using the EasySep ™ Human CD4+ T-cells isolation kit (Stem Cell Technologies, Canada). Briefly ...