Labshake search
Citations for Stemcell Technologies :
1251 - 1300 of 1500 citations for Mouse MBL2 Binding Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... 100μL of Isolation Cocktail and 100μL of Rapid Spheres (Easy Sep™ Direct Human Total Lymphocytes Isolation Kit: 19655, StemCell Technologies) were added to the remaining whole blood ...
-
bioRxiv - Immunology 2021Quote: ... plasmablasts were enriched from the whole blood (day 7 after second vaccination) by negative selection using custom direct human plasmablasts isolation kit containing paramagnetic beads and antibodies for negative selection (StemCell Technologies). Enriched cells were stained 30 min on ice in a RoboSep buffer (StemCell Technologies ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were enriched from whole blood (day 10 after first, and day 7 after second vaccination) using direct PBMCs isolation kit (StemCell Technologies). For singe-cell antibody secretion and paired antibody sequencing studies ...
-
bioRxiv - Immunology 2020Quote: Monocytes were enriched from the PBMCs of healthy individuals by using the EasySep CD14 positive selection kit according to the manufacturer’s instructions (STEMCELL Technologies, Canada). Monocytes were cultured in the DC medium for 6 days ...
-
bioRxiv - Immunology 2020Quote: ... Total CD3+ T cells were isolated from unfractionated PBMCs using the EasySep™ Human T Cell Isolation Kit (Stem Cell Technologies) with a DynaMag™-2 magnet (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... Preliminary experiments were also performed after isolation of monocytes using the EasySep human monocyte enrichment kit with CD16 depletion (StemCell Technologies) and the EasySep human monocyte isolation kit (StemCell Technologies ...
-
bioRxiv - Immunology 2021Quote: ... In vitro experiments were performed using human plasmacytoid dendritic cells (pDCs) purified from PBMCs by negative selection with the EasySep Human Plasmacytoid DC enrichment kit (STEMCELL Technologies). pDCs were cultured in RPMI 1640 (Invitrogen ...
-
bioRxiv - Immunology 2020Quote: ... Memory human CD8+ T cells were purified from healthy donor blood samples using the EasySep Human Memory CD8+ T cell enrichment kit (StemCell Technologies), cells were routinely ∼90% CD8+RO+RA-.
-
bioRxiv - Immunology 2020Quote: Total human CD8+ T cells were purified from healthy donor blood samples using the EasySep Negative human CD8+ T cell isolation kit (StemCell Technologies). CD8+ T cells were routinely ∼90% pure ...
-
bioRxiv - Immunology 2020Quote: ... Total mDC and pDC were negatively enriched from PBMCs using the EasySep™ Human Pan-DC Pre-Enrichment Kit (StemCell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... Total T-cells were purified from the same donor’s PBMC using the EasySep™ Human T cell Isolation kit (StemCell Technologies). Purity of total CD4+ and CD8+ T-cells was ∼94% (Supplementary Fig 2) ...
-
bioRxiv - Cell Biology 2020Quote: Peripheral blood was collected from a healthy volunteer into a BD Vacutainer tube and mononuclear cells were isolated using the SepMate kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2021Quote: Human ES cells were differentiated using the STEMdiff Definitive Endoderm Kit (TeSR-E8 Optimized) from STEMCELL Technologies (Ramme et al. 2019). Rat ES cells were differentiated following an optimized mouse protocols (Kim et al ...
-
bioRxiv - Cancer Biology 2020Quote: ... WT or Rnf5−/– Lin−Sca1+c-Kit+ cells transformed with GFP-MLL-AF9 were resuspended in methylcellulose M3234 (Stem Cell Technologies) supplemented with 6 ng/mL IL-3 ...
-
bioRxiv - Immunology 2022Quote: Splenic macrophages were magnetically labeled and isolated using StemCell EasySep PE Positive Selection Kit II in concert with an F4/80 PE-conjugated antibody (StemCell Technologies). Briefly ...
-
bioRxiv - Immunology 2022Quote: ... NK cells were either isolated from cryopreserved PBMCs by either negative selection using the EasySep NK cell enrichment kit (StemCell technologies) or by depletion of T cells with human CD3 MicroBeads (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
-
bioRxiv - Immunology 2022Quote: ... Bone marrow plasma cells (BMPC) were enriched from bone marrow mononuclear cells using the EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% DMSO in FBS.
-
bioRxiv - Molecular Biology 2022Quote: ... Neutrophils were isolated from 20 ml of blood with EasySep™ Human Neutrophil Isolation Kit (STEMCELL Technologies, British Columbia, Canada, 19666) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... apoptotic and dead cells were removed using immunomagnetic cell separation with the Annexin Dead Cell Removal Kit (StemCell Technologies, Cat# 17899) and EasySepTM Magnet (StemCell Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
-
bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
-
bioRxiv - Microbiology 2022Quote: ... Loosely adherent immature BMDCs were collected and then CD11c+ DCs were enriched using CD11c positive selection kit (Stemcell Technologies, Cambridge, MA). Twenty-four hours after culture of purified subsets ...
-
bioRxiv - Microbiology 2022Quote: ... primary CD4+ T-cells were negatively enriched using EasySep Human CD4+ T-cell isolation kit (Catalog no. 17952; Stem Cell Technologies) and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2 ...
-
bioRxiv - Microbiology 2024Quote: Human polymorphonuclear neutrophils (PMNs) were isolated from the venous blood of healthy individuals following informed consent using a STEMcell isolation kit (STEMCELL Technologies). The purified neutrophils were resuspended in RPMI medium ...
-
bioRxiv - Molecular Biology 2022Quote: H9 human ES cell lines obtained from the HMS Cell Biology Initiative for Genome Editing and Neurodegeneration were cultured using the mTeSR1TM1 kit (STEMCELL technology) and 1% penicillin-streptomycin (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... CD4+ T cells were negatively selected from single cell suspensions using the EasySep™ CD4+ T cell isolation kit (Stemcell Technologies) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Spleens were processed into single cell suspensions and CD4+ T cells were isolated using the EasySep CD4+ T cell isolation kit (StemCell Technologies) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Human naïve T cells were isolated from PBMCs using the EasySep™ Human naïve CD4+ T Cell Isolation Kit Human (Stemcell Technologies) as per the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: NK cells were obtained from anonymous buffy coats (Karolinska Hospital, Huddinge, Sweden) by negative selection using EasySep Direct Human NK cell isolation kit (Stemcell Technology) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
-
bioRxiv - Neuroscience 2024Quote: ... we took advantage of the STEMdiff™ trilineage differentiation Kit and followed the manufacturer’s instructions with minor modifications (Cat Nº 05230, Stemcell technologies). hiPSCs were cultured onto Matrigel-coated glass coverslips and on day 0 ...
-
bioRxiv - Neuroscience 2024Quote: ... 70,000-80,000 EPSCs were used for the development of individual organoids using the STEMdiff™ Cerebral Organoid Kit (Stemcell technologies, #08570) and the STEMdiff™ Cerebral Organoid Maturation Kit (Stemcell technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... mature NK cells were isolated from peripheral blood mononucleated cells with the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies). The human mesenchymal stromal cells (h-MSC ...
-
bioRxiv - Immunology 2024Quote: B cells were isolated from blood of convalescent COVID-19 donors using the EasySep B kit (Stemcell Technologies, cat no 17954). SARS-nCoV-2 Spike RBD (Raybiotech ...
-
bioRxiv - Immunology 2024Quote: ... Positive selection of CD34+ cells was then performed on the low-density cells using the EasySep™ kit from STEMCELL Technologies) to obtain final CD34+ purities ...
-
bioRxiv - Immunology 2024Quote: ... Single cell suspensions were generated and enriched for CD4+ T cells by negative selection using EasySep magnetic bead kit (STEMCELL Technologies) and stained with anti-mouse CD4 (RM4-5 ...
-
bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated by negative selection using the EasySep Human CD8 negative isolation kit (StemCell Technologies, Vancouver, BC).
-
bioRxiv - Cancer Biology 2023Quote: ... Further T cells isolation from human PBMCs was done with EasySep™ Human T Cell Isolation Kit (STEMCELL Technologies, 100-0695) according to the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2023Quote: Patterned differentiation of iPSCs toward neural progenitor cells (NPCs) was conducted using dual-SMAD inhibition (STEMdiff SMADi Neural Induction Kit, STEMCELL Technologies). To promote complete and uniform generation of NPCs ...
-
bioRxiv - Molecular Biology 2022Quote: ... density gradient centrifugation and total CD4+ T cells were enriched by negative selection with the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies) to an average purity of 90% ...
-
bioRxiv - Molecular Biology 2023Quote: ... CD4 T cells were isolated from PBMCs by negative selection using EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, #19052). All participants signed informed consent forms approved by the Ethics Committee of the Ghent University Hospital (Belgium ...
-
bioRxiv - Biochemistry 2023Quote: CD8+ T lymphocytes were enriched from lung cell suspensions prepared according to the manufacturer’s instructions with the CD8+ T cell isolation kit (Stem Cell Technologies). Purified CD8+ T cells were incubated in ELISpot plates in the presence of medium or a pool of S1 peptides (1 μg/mL ...
-
bioRxiv - Developmental Biology 2023Quote: ... they were collected and moved to a new 96-well plate containing 150 µl of induction media (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) per well ...
-
bioRxiv - Developmental Biology 2023Quote: ... 70 µl of media were removed from each well and replaced with 150 µl expansion medium (Cerebral organoid kit, cat. n. 08570, StemCell Technologies). On day 10 ...
-
bioRxiv - Cancer Biology 2023Quote: MDSCs were generated from healthy human donors by differentiation of CD14+ monocytes isolated using a RosetteSep human monocyte enrichment kit (STEMCELL Technologies). In order to generate MDSCs ...
-
bioRxiv - Immunology 2023Quote: hESCs were differentiated into hematopoietic progenitors using either an embryoid body culture described previously174 or the STEMdiff Hematopoietic Kit (STEMCELL Technologies) per the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... Human T cells were purified (> 96% purity) by magnetic negative selection using an EasySep Human T Cell Isolation Kit (Cat#17951, Stemcell Technologies) according to the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2023Quote: ... For microglial differentiation iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (05310, Stem cell Technologies). HPCs were either frozen using Bambanker HRM freezing media (BBH01 ...