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Citations for Stemcell Technologies :
1201 - 1250 of 2404 citations for Human Probable fructose 2 6 bisphosphatase TIGAR TIGAR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Mammary organoids from eutherian species were cultured in Intesticult human organoid growth media (StemCell Technologies, Vancouver, Canada, Cat# 06010). Mammary organoids of opossum were cultured in either Intesticult ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured on recombinant human VTN (rhVTN) coated 60mm plates in StemFlex media (Stem Cell Technologies, cat. A3349401) and passaged using CTS EDTA Versene Solution (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells from healthy donor and HSCT recipients were labeled with Rosettesep human monocyte enrichment cocktail (Stemcell Technologies, Cambridge, MA) and centrifuged through Histopaque density gradient medium (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Human pan T cells were purified from frozen peripheral blood mononuclear cells (PBMCs, STEMCELL Technologies, Cat #70025.2. Lot 201671701C) using a negative selection human Pan T Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... were seeded (70,000 cells each) in medium for long-term culture of human cells (catalogue no. 05150; STEMCELL Technologies) supplemented with hydrocortisone in collagen-coated plates ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Microbiology 2023Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
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bioRxiv - Neuroscience 2023Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Human embryonic or induced pluripotent stem cells were cultured in the commercially defined pluripotency maintaining media mTeSR1 (StemCell Technologies) on Cultrex (R&D Systems)-coated tissue culture plates ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
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bioRxiv - Cell Biology 2024Quote: ... H1 human embryonic stem cells (WiCell, WA01) were routinely cultured as colonies in mTeSR Plus (STEMCELL Technologies, 100-0276) with 0.2% penicillin/streptomycin (BioConcept ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected with 2 mL mTeSRplus (Stem Cell Technologies, 05825), span down and resuspended in 1 mL mTeSRplus ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellet is resuspended in 2-5ml accutase™ (StemCell Technologies, 07920), depending on the sample amount ...
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bioRxiv - Cancer Biology 2022Quote: ... PHA was washed and IL-2 (50 U/ml, StemCell Technology) was added to the medium and replaced every 2 days for a time period of 5 days.
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bioRxiv - Neuroscience 2023Quote: ... Each plate was then split 1:2 using ReLeSR (StemCell Technologies), with half of the cells used for DNA extraction for Sanger sequencing ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... 2% BSA (Miltenyi) and 10 uM ROCK inhibitor (StemCell technologies 72302). For all wash steps ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% Neurocult SM1 Neuronal Supplement (StemCell Technologies; cat. #05711), 1% horse serum (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
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bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
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bioRxiv - Molecular Biology 2023Quote: The 4th mammary glands were harvested from 6-8-week-old female mice from desired genotype and incubated in Collagenase/Hyaluronidase medium (Stem Cell Technologies, #07912) and shaken at 37°C for 2h ...
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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bioRxiv - Bioengineering 2023Quote: ... Thawed PMBCs from a vial containing around 1 × 107 cells were pre-cultured in one well of low attachment 6-well plate including 4 mL of StemSpan-AOF (Cat#ST100-0130, STEMCELL Technologies, Vancouver, Canada) supplemented with recombinant human IL-6 (100 ng/mL) ...
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bioRxiv - Bioengineering 2024Quote: Two days post-electroporation 500 HSPCs were plated in each well of a SmartDish 6-well plate (STEMCELL Technologies, Vancouver, Canada, cat.: 27370) containing MethoCult H4434 Classic (STEMCELL Technologies ...
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bioRxiv - Genetics 2023Quote: ... The remaining positive cells were bulk sorted in a single well of a Vitronectin coated 6-well plate containing Essential 8 medium and ROCK inhibitor (STEMCELL Technologies, Cat#72304) for the purpose of measuring editing efficiency.
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bioRxiv - Developmental Biology 2024Quote: ... the cells were plated in 6-well plates using mTeSR media supplemented with 10 µM Y-27632 (Stem Cell Technologies, catalog no. 72304) for 48 hours ...
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bioRxiv - Genomics 2024Quote: ... for 6 days for patterning into neuronal precursors and then placed in BrainPhys Neuronal Medium + Maturation supplement (STEMCELL Tech, catalog # 05797 + catalog # 08606) for up to 43 days ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at a high density of (250kcells/cm2 in 6-well plates) as a single-cell suspension in StemDiff Neural Induction Media with Dual SMAD Inhibition (STEMCELL Technologies cat #08581), supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies cat #72304) ...
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bioRxiv - Synthetic Biology 2024Quote: ... genome-edited iPSCs were plated at a density of 250 cells per well of a 6-well plate in mTeSR1 medium supplemented with 1x CloneR2 reagent (cat.: 100-0691; STEMCELL Technologies, Vancouver, Canada). After 48h ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Cancer Biology 2022Quote: ... DNTs were enriched by depleting CD4+ and CD8+ cells using RosetteSep™ human CD4- and CD8-depletion cocktails (Stemcell Technologies). The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3 ...
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bioRxiv - Microbiology 2022Quote: ... Monocytes were isolated from whole blood by negative selection using RosetteSep human monocyte enrichment cocktail following the manufacturer’s protocol (StemCell Technologies). Using density gradient medium (Lymphoprep ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... human iPSCs were seeded on a matrigel coated dish and cultured in the STEMdiff SMADi Neural Induction medium (STEMCELL Technologies) for 7 days34 ...
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bioRxiv - Cell Biology 2020Quote: For structured illumination microscopy primary NK cells were enriched from peripheral blood of healthy human donors using RosetteSep (Stemcell Technologies). Freshly isolated cells were pre-incubated with anti-CD56 Alexa Fluor 647 (clone HCD56 ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
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bioRxiv - Cell Biology 2022Quote: hematopoietic progenitors (CD34+CD43+ for iPSC and CD34+ for primary cells) were plated in human methylcellulose medium H4434 (STEMCELL technologies), containing recombinant human cytokines and scored for the presence of colonies 14 days after.
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Cancer Biology 2024Quote: Freshly isolated human HSPCs were plated at 200,000 cells/ml and cultured in IMDM medium supplemented with 25% BIT9500 (StemCell Technologies), 1% penicillin/streptomycin (P/S) ...
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in a 1:1 ratio of DMEM-F12 and NeuroCult NS-A Basal Medium (Human) (Stem Cell Technologies) with 1X N-2 supplement (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from human donor leukopaks by gradient centrifugation on lymphoprep (cat#07861, Stemcell Technologies). Thereafter ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Developmental Biology 2023Quote: ... HUCB CD34+ cells harvested from Matrigel Matrix were subsequently enriched using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). Enriched CD34+ were then either used for FACS ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...