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Citations for Stemcell Technologies :
1201 - 1250 of 1366 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 1 million cells were cultured for 8 days in StemSpanTM SFEM II medium (Stem Cell Technologies Catalog #09605) supplemented with 1X StemSpanTM Erythroid Expansion Supplement (Stem Cell Technologies Catalog #02692).
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bioRxiv - Bioengineering 2024Quote: ... 500 and 1500 CD34+ cells were added to 1 mL of MethoCult™ H4034 Optimum medium (StemCell Technologies) and placed into one well of a 6-well plate ...
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Matrigel-coated dishes using either mTeSR-1 or TeSR-E8 medium (Stem Cell Technologies). The medium was replaced daily until cells get 70-80% confluence ...
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies) followed by Trypsin-EDTA (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 1×10^5 cell per ml were plated in triplicate into M3234 incomplete methylcellulose medium (StemCell Technologies) supplemented with 0.1 ng/mL GM-CSF (Peprotech) ...
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bioRxiv - Physiology 2024Quote: ... and seeded onto Matrigel (Corning)-coated p60 dishes at a density of ∼1 × 106 cells/dish in mTeSR™ plus medium (STEMCELL Technologies) supplemented with 10 μM ROCK inhibitor Y-27632 (Selleckchem) ...
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bioRxiv - Bioengineering 2024Quote: hESCs were diluted to 1 cell/100 µL in MTeSR Plus media supplemented with 1x CloneR (STEMCELL Technologies) and plated into two 96-well plates per sample ...
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bioRxiv - Cell Biology 2024Quote: ... Long-term feeder free culture of ERCi003-A iPSCs was conducted in mTeSR™1 (Stem Cell Technologies) under 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the culture medium was supplemented with 1 mM EDTA and 10 μM Y-27632 (StemCell Technologies, Vancouver, Canada), and cells were allowed to incubate for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... HSPCs (Figure 1 – figure supplement 1a) were sorted and transduced with the barcode library in StemSpanMedium SFEM (STEMCELL Technologies) with 50 ng/ml mSCF (STEMCELL Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... In short, TJP1-GFP(ZO-1-GFP) WTCs (Allen Institute, Seattle, WA) were plated in TeSR (Stem Cell Technologies) at 200,000 cells/well of Matrigel-coated 24-well plates on day (−1) ...
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bioRxiv - Neuroscience 2020Quote: Differentiation into cerebral organoids was performed with a commercially available differentiation kit from Stemcell Technologies (Catalog number 08570-1) based on previously reported protocols [8 ...
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bioRxiv - Microbiology 2020Quote: Human iPSC (XCL-1)98-derived neural progenitor cells (hNPCs) were purchased commercially (StemCell Technologies, Cat #70901, Lot #17080) and maintained up to ten passages according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Cancer Biology 2021Quote: ... Following enzymatic digestion of tumor tissues using 1x Collagenase/Hyaluronidase and DNase-1 (Cat # 07912 & 07900; Stem cell technologies), dissociated tumor cells were collected in a different tube and filtered through 40 µ strainer ...
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bioRxiv - Systems Biology 2023Quote: ... TRA-1-60) as well as differentiation into the three germ layers (STEMdiff Trilineage Differentiation kit, #05230, StemCell Technologies).
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended at a concentration of 1×106 cells/mL in neuron media [Brainphys (StemCell Technologies #05790), 1×N2 (ThermoFisher #17502-048) ...
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bioRxiv - Cell Biology 2023Quote: ... 1% (v/v) B27™ minus insulin) supplemented with 5 µM CHIR99021 and 33.3 ng Activin A (STEMCELL Technologies). Medium was exchanged daily without CHIR99021 supplementation ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD11B-FITC (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced with 1 mL of BrainPhys neuronal medium containing SM1 neuronal supplement (ST-05792, STEMCELL technologies) and Human iPSC-derived mature astrocytes (XCL-1 mature astrocytes ...
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bioRxiv - Bioengineering 2024Quote: HSPCs were plated at a concentration of 1 × 103 cells/ml in a methylcellulose-containing medium (GFH4435, STEMCELL Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Immunology 2024Quote: ... T cells were plated at 1 million cells/mL in either ImmunoCult XF T cell Expansion Medium (STEMCELL Technologies) supplemented with 200U/mL IL-2 (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... The Plating medium was consisted of 1 × BrainPhys Neuronal Medium/N2-A/SM1 kit (BrainPhys; Stem Cell Technologies, 05793) supplemented with 10 μM Y27632 ...
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bioRxiv - Neuroscience 2024Quote: ... the plating medium was removed and replaced by Neurobasal medium supplemented with SM1 supplement (StemCell Technologies; 1:50 dilution), 0.5 mM glutamine and 0.12 mg/mL gentamycin ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were seeded at a density of 1.6-5.8 × 104 cells/cm2 on plates coated with a 1:30 dilution of Matrigel in mTeSR+ (STEMCELL Technologies) supplemented with 100 U/mL penicillin-streptomycin and 5 µM ROCK inhibitor Y-27632 ...
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bioRxiv - Immunology 2021Quote: ... inguinal lymph nodes from immunized or naïve mice or live LN sections were incubated in 1 mL of digestion solution comprised of RPMI1640 medium containing 0.8 U/mL Dispase (07913, Stemcell Technologies), 0.1 mg/mL Collagenase D (11088858001 ...
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bioRxiv - Cell Biology 2021Quote: ... Rescue of epithelial integrity was induced by threating hiPSCs for 1 day with LPA (StemCell Technologies, ref. 72694, 5 µM). Chemical disruption of epithelial integrity was induced by threating hiPSCs for 1 day with Blebbistatin (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... The yolk sac embryoid bodies (YS-EBs) were generated by treating the YS-EBs with mTeSR 1 media (STEMCELL Technologies) supplemented with bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Cancer Biology 2021Quote: CD34+ cells isolated from MF patients were plated at a density of 500 cells/replicate in 30-mm dishes containing 1 mL of StemSpan SFEM with 1.1% methylcellulose (Cat# H4230, StemCell Technologies), to which SCF ...
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bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... derived from different mice using a previously described method23) and selected for genomic integration using puromycin (2.5 μg mL-1; StemCell Technologies) using established procedures28 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD34-PE (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Immunology 2024Quote: ... Freshly harvested organ tissue from male and female bats was washed in PBS and cut into 3-5 mm small pieces before placing up to four individual pieces into a 1 ml cryostorage tube containing 0.5ml-1ml of Cryostore10 freezing medium (Stemcell Technologies). Vials were immediately transferred to a cryostorage container (Mr ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 of sorted lentivirally transduced CD34+ HSPCs were seeded into 1 mL of MethoCultTM H4434 Classic media (STEMCELL Technologies #04434) supplemented with StemSpan™ CC100 with antibiotics ...
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bioRxiv - Neuroscience 2024Quote: ... The yolk sac embryoid bodies (YS-EBs) were generated by treating the YS-EBs with mTeSR 1 media (STEMCELL Technologies) supplemented with bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Immunology 2022Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... FITC-conjugated mouse IgG2b anti-human CD32 Ab (clone IV.3, cat. no. 60012Fl.1, StemCell Technologies, Vancouver, BC, Canada). Mouse isotype controls APC-conjugated mouse IgG2a ...
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bioRxiv - Cell Biology 2024Quote: ... CS-001) were cultured on Matrigel-coated plates in feeder-free conditions in mTeSR-1 medium (Stemcell Technologies, Vancouver, Canada). As schematized in Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... on Vitronectin (GibcoTM Cat#A14700, VTN-N) coated dishes and in mTESR1 (Stem Cell Technologies Cat#85850, mTeSR™1) on Matrigel (Corning Cat#354277 ...
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bioRxiv - Cancer Biology 2024Quote: ... or 1 mM db-cAMP (from 500 mM stock in 50/50 in Ethanol/PBS) (STEMCELL Technologies, Vancouver, BC, Canada), 0.5 mM 3-Isobutyl-1-methylxanthine ...
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bioRxiv - Cancer Biology 2021Quote: ... Patient sample mononuclear cells were cultured in RPMI with 10% FBS and 50% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 uM UM729 (StemCell Technologies).
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bioRxiv - Genomics 2021Quote: ... they were seeded at approximately 30% confluency in Matrigel-coated culture dishes and cultured in mTeSR™1 (85851, STEMCELL Technologies) until cells fully adhered to the plate (at least 3 hours) ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...