Labshake search
Citations for Stemcell Technologies :
1151 - 1200 of 1553 citations for Mouse Anti Human IgG Fab Alexa Fluor 594 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2024Quote: ... mouse NGN2 at the AAVS1 locus and pC13N-dCas9-BFP- KRAB at human CLYBL intragenic safe harbor locus (between exons 2 and 3) were cultured in mTeSR Medium (Stemcell Technologies; Cat. No. 85850) on Corning Tissue Culture Treated Dishes (VWR International LLC ...
-
bioRxiv - Microbiology 2024Quote: ... Monolayer cultures were seeded into 96-well plates using a 1:1 ratio of IntestiCult™ Organoid Growth Medium Human Basal Medium (StemCell Technologies, 100-0190) and Organoid Supplement (StemCell Technologies ...
-
bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
-
bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
-
bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
-
bioRxiv - Cancer Biology 2024Quote: Primary human CD4 and CD8 T cells were isolated from PBMCs by negative selection using STEMCELL EasySep isolation kits (Stemcell Technologies, 17952 and 17953), and mixed 1:1 ...
-
bioRxiv - Biochemistry 2024Quote: ... PBMCs were isolated from the blood of healthy volunteer and enriched for CD3+ Tcells using EasySep™ human T-cell isolation kit (Stemcell Technologies, Vancouver,Canada) as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Hematopoietic stem cells were enriched using the EasySep Mouse Hematopoietic Progenitor Cell Enrichment kit (Stemcell Technologies) and 1*106 HSCs were administered via tail vein injection to recipient mice ...
-
bioRxiv - Immunology 2020Quote: Isolated IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were isolated using the EasySep Mouse CD4+ T-cell isolation kit (Stemcell Technologies). The CD4+ T cells were then added in a 1:1 ratio to cultured CMT167 cells ...
-
bioRxiv - Immunology 2021Quote: ... S3d) for the studies involving glucose exposure using EasySep™ Mouse Neutrophil Enrichment Kit (STEMCELL Technologies), as per manufacture’s protocol and as described previously (25 ...
-
bioRxiv - Genomics 2019Quote: Mouse bone marrow cells (2.5×104) suspended in methylcellulose semisolid medium (Methocult M3231) (Stem Cell Technologies) were plated in 35-mm culture dishes in the presence of 0.5 % FBS ...
-
bioRxiv - Immunology 2021Quote: ... Using EasySep mouse B cell enrichment kit and manufacturer’s protocol (StemCell Technologies Inc., Tukwila, WA, USA), B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum ...
-
bioRxiv - Immunology 2022Quote: NK cells from spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... All CD19+ B cells were isolated (STEMCELL Technologies EasySep Mouse B Cell Isolation kit, STEMCELL Technologies) and mRNA was extracted and purified (PureLink RNA Mini Kit ...
-
bioRxiv - Immunology 2021Quote: ... EasySep Mouse Naive CD4+ T cell and CD8+ T cell Isolation Kit (StemCell Technologies, Vancouer, Canada) were purchased.
-
bioRxiv - Immunology 2022Quote: B cells from spleen were sorted with EASYSEP MOUSE B cell isolation kit (Stem Cell Technologies) and lysed using RIPA buffer (Santa Cruz ...
-
bioRxiv - Immunology 2023Quote: ... Dendritic cells were isolated with the EasySepTM Mouse Pan-DC Enrichment Kit II (Stem Cell Technologies). T Cells were isolated with the EasySepTM Mouse T Cell Isolation Kit (Stem Cell Technologies) ...
-
bioRxiv - Immunology 2024Quote: ... CD8+ T cells were isolated using the EasySep Mouse CD8+ T cell isolation kit (StemCell Technologies) and resuspended at a concentration of 106 cells/mL in complete RPMI supplemented with 1X sodium pyruvate (ThermoFisher) ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... then DCs were isolated using the EasySep™ Mouse CD11c Positive Selection Kit II (STEMCELL Technologies). While adding the CD11c selection antibody cocktail to the cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lineage-negative cells were enriched using EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s protocol prior to sorting ...
-
bioRxiv - Cancer Biology 2023Quote: ... and T lymphocytes purified using Easy SepTM Mouse T cell isolation kit (STEMCELL Technologies, Cat# 19851A), as we described83 ...
-
bioRxiv - Cancer Biology 2023Quote: ... HSCs were enriched using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Total CD4 T cells were isolated using EasySep mouse CD4+ T cell isolation kit (Stemcell technologies) according to manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2024Quote: ... then dissociated and Cd4+/CD8+ T cells were isolated using EasySepTM Mouse isolation kit (Stemcell Technologies) according to manufacturer’s instructions.
-
bioRxiv - Immunology 2024Quote: ... and polarized following the manufacturer’s protocol for Immunocult Mouse Th1 or Th2 differentiation supplements (STEMCELL Technologies). For Th17 polarization ...
-
bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: Flow cytometer-sorted murine or human HSCs were resuspended in 4 mL complete MethoCult GF M3434 medium or MethoCult™ H4435 Enriched (STEMCELL Technologies, 03434 and 04435) thawed at RT and 1 mL of the suspension was placed into 35 mm dishes in triplicates ...
-
bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
-
bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
-
bioRxiv - Immunology 2021Quote: Untouched total T or isolated CD4+ or CD8+ T cells were purified from buffy coats or whole blood with RosetteSep Human T or CD4+ or CD8+ T cell enrichment cocktails (STEMCELL Technologies, Cat#: 15061, 15062, 15063). Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... the remaining fresh murine BM cells were purified using human CD45 MicroBeads (Miltenyi Biotech) and cryopreserved in CryoStor® CS5 (STEMCELL Technologies, Cambridge, MA, USA). For secondary transplantation ...
-
bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: The first step consists in a CTC enrichment from whole blood sample by immunomagnetic negative selection using the EasySep™ Direct Human CTC Enrichment Kit (StemCell Technologies®, Vancouver, Canada) according to manufacturer instruction ...
-
bioRxiv - Bioengineering 2024Quote: ... PBMC samples were thawed on the day of activation and T cells were purified via the EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies Inc, cat#19053). Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc ...
-
bioRxiv - Cell Biology 2024Quote: Primary human T cells were isolated from the peripheral blood of a healthy donor using a negative selection kit (EasySep, STEMCELL Technologies Canada Inc., Vancouver, BC) and used for testing the sensitivity of the lifetime flow system to T cell activation ...
-
bioRxiv - Immunology 2021Quote: ... isolated T-IEL were further enriched using an EasySep™ Mouse CD8α positive selection kit (STEMCELL technologies) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... B cells were purified using the EasySep negative selection mouse B cell enrichment kit (Stem Cell Technologies). B cell purity was assessed by flow cytometry using anti CD19 or B220 antibodies (we routinely observed over 98% purity) ...
-
bioRxiv - Immunology 2019Quote: ... hMT and s-hMT mice were purified with the EasySep Mouse B Cell Isolation Kit (Stemcell Technologies). B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
-
bioRxiv - Genomics 2020Quote: ... and lineage negative cells were isolated using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies).
-
bioRxiv - Immunology 2019Quote: ... and NK cells were purified by negative selection using EasySep Mouse NK Cell Isolation Kit (STEMCELL TECHNOLOGIES). Normalized NK-cell numbers (1–3 × 106 ...
-
bioRxiv - Immunology 2021Quote: T cells were isolated from disaggregated synovium with the EasySep Mouse T cell isolation kit (STEMCELL Tech) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... T cells were purified by negative selection with the EasySep Mouse T cell isolation kit (STEMCELL Tech) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: NK cells from the spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Our murine neurosphere media consisted of 45 ml Neurocult Mouse Basal media (Stem Cell Technologies, Catalog# 05700), 5 ml of mouse Neurocult Proliferation Supplement (Stem Cell Technologies ...