Labshake search
Citations for Stemcell Technologies :
1101 - 1150 of 1444 citations for Recombinant Human TIMP Metallopeptidase Inhibitor 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
-
bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
-
bioRxiv - Biophysics 2024Quote: NK cells were obtained from anonymous buffy coats (Karolinska Hospital, Huddinge, Sweden) by negative selection using EasySep Direct Human NK cell isolation kit (Stemcell Technology) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
-
bioRxiv - Genomics 2019Quote: ... iPSCs were dissociated with 1:3 Accutase and plated onto 24-well plates pre-coated with 0.5% GelTrex in mTeSR1 supplemented with 10uM Y-27632 ROCK Inhibitor (STEMCELL Technologies). 24 hours later another layer of GelTrex at 1.5% was added in mTeSR1 media ...
-
bioRxiv - Developmental Biology 2021Quote: ... colonies were disaggregated into single cells using Accutase and plated in the presence of Rock Inhibitor (StemCell Technologies Y-27632) on matrigel-coated plates ...
-
bioRxiv - Developmental Biology 2022Quote: ... cultured in either H9 hESC or DEF-CS media for 48 hours with 10µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for first 24 hours and maintained in 20% O2/5% CO2/37°C ...
-
bioRxiv - Genomics 2019Quote: ... and quenched with complete STEMflex media containing 10-µM RHO/ROCK pathway inhibitor (Ri) Y-276932 (STEMCELL Technologies Cat#72304). Cells were subsequently spun down at 300 rcf for 5 minutes and resuspended in media supplemented with Ri ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1x penicillin-streptomycin and 10ng/ml FGF2 (Department of Biochemistry, University of Cambridge) and 10µM ROCK inhibitor Y-27632 (72304, STEMCELL Technologies). For RSeT cells ...
-
bioRxiv - Bioengineering 2023Quote: ... Embryoid bodies formed via hanging drop approach with each droplet containing 3-4×104 human single PSCs in 20 μl were cultured hanging on TESR-E8 supplemented with Polyvinyl Alcohol (PVA) at 4mg/ml (SigmaAldrich) and rock inhibitor (Y-27632) at 10μM (StemCell Technologies) at the lid of Petri dishes ...
-
A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... counted and added at 1.2 million cells per microwell in AggreWell media containing 10 μM ROCK inhibitor (StemCell technologies 72302). Media was carefully changed the following day to remove ROCK inhibition ...
-
bioRxiv - Genomics 2023Quote: ... resuspending cell pellet in mTeSR Plus Basal Medium supplemented with 0.1% dihydrochloride ROCK Inhibitor (Stemcell technologies; Cat. No. Y-27632), counting and plating onto Geltrex-coated plates at the desired number.
-
bioRxiv - Neuroscience 2023Quote: ... was pipetted into the cuvette and incubated for 10 minutes at 37°C and then transferred to a fresh well of a Matrigel coated 6-well plate with 2ml of mTeSR1 supplemented with ROCK-inhibitor (10μM, StemCell Technologies). Single GFP-positive cells were sorted in a 96-well plate with mTeSR plus CloneR (StemCell Technologies) ...
-
bioRxiv - Cell Biology 2019Quote: ... In either situation cells were resuspended in APEL 2 medium (Stem Cell Technologies) containing 10µM ROCKi ...
-
bioRxiv - Microbiology 2021Quote: ... About 2×105 cells were washed twice in Robosep buffer (#20104, Stemcell Technologies) and fixed in 300 μl CellFix buffer 1X (#340181 ...
-
bioRxiv - Neuroscience 2021Quote: ... medium was replaced with 2 mL/well iHSC Medium B (StemCell Technologies #05310). An additional 1 mL/well of iHSC Medium B was added every other day ...
-
bioRxiv - Cancer Biology 2020Quote: ... thawed organoids were rinsed with 2% fetal bovine serum (FBS, from STEMCELL Technologies) in Hank’s Balanced Salt Solution (HF) ...
-
bioRxiv - Genetics 2020Quote: ... Expansion phase medium for HUDEP-2 cells consists of SFEM (Stemcell Technologies #09650) base medium supplemented with 50 ng/ml recombinant human SCF (R&D systems #255-SC) ...
-
bioRxiv - Genomics 2020Quote: HUDEP-2 cell lines were cultured in StemSpan™ SFEM II (STEMCELL Technologies) along with 50 ng/ml SCF (ImmunoTools) ...
-
bioRxiv - Genomics 2021Quote: ... 2×107 T cells were aliquoted into CryoStor cryopreservation media (Stem Cell technologies) for long-term storage.
-
bioRxiv - Neuroscience 2023Quote: ... and PINK1 p.I368N-2# (1067#) were cultured using mTeSR (STEMCELL Technologies, Catlog #85850) media on Matrigel (Corning ...
-
bioRxiv - Immunology 2023Quote: ... Isolated cells were cultured for 1-2 weeks in StemSpan Medium (Stemcell Technologies) supplemented with 10 ng/ml interleukin (IL)-3 ...
-
bioRxiv - Cancer Biology 2023Quote: ... the media was supplemented with 2 μg/ml heparin (STEMCELL Technologies; Cat #07980). When cells reached confluency ...
-
bioRxiv - Neuroscience 2023Quote: ... with 2 % (v/v) of SM1 Neuronal Supplement (5711, STEMCELL Technologies Inc., Germany) and 0,5 % (v/v ...
-
bioRxiv - Neuroscience 2024Quote: ... Wash Solution 2 was composed of 96% BrainPhys Neuronal Medium (StemCell Technologies: 5790), 1X N2 (ThermoFisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
-
bioRxiv - Immunology 2021Quote: ... CECs were isolated from human bone marrow aspirates or PBMC derived CECs culture using EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
-
bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
-
bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
-
bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
-
bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF human iPSCs were seeded in a Matrigel-coated T25 flask in mTeSR1 medium (Stem Cell Technologies, #85870) with ROCK inhibitor ...
-
bioRxiv - Bioengineering 2021Quote: ... CFU-E and CFU-GEMM Colony identification was performed 14 days after seeding in MethoCult media according to the Atlas of Human Hematopoietic Colonies (StemCell Technologies, 28700).
-
bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coat (Research Blood Components) using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
-
bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
-
bioRxiv - Cancer Biology 2021Quote: CD138+ myeloma cells were isolated from patient peripheral blood using a magnetic bead-based approach (EasySep Human CD138 Positive Selection Kit, StemCell Technologies 17877). The CD138+-enriched sample was titrated into 2.5e7 ...
-
bioRxiv - Immunology 2022Quote: ... the Negative Selection EasySep CD4+ T cell kit (17951) or RosetteSep Human CD4+ Cell Isolation Kit (15062) from Stemcell Technologies (Vancouver, Canada) according to the manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... The crypts were embedded in 30 μl of Matrigel and cultured with Human IntestiCult™ Organoid Growth Medium (OGM) (Basal Medium and Organoid Supplement, Stemcell Technologies) supplemented with 100 IU Penicillin and 100 μg/ml Streptomycin (Corning ...
-
bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
-
bioRxiv - Cancer Biology 2022Quote: Differentiated human and murine H3K27M glioma cells were generated by dissociating the respective gliomaspheres into single cells with Accutase (Stemcell Technologies; # 07920) and subsequently cultured and maintained in the respective base media supplemented with 10% FBS for 14 days to generate a monolayer adherent culture ...
-
bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: CD4 T cells were isolated from PBMC by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, 19052). Purity was typically >98%.