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Citations for Stemcell Technologies :
1101 - 1150 of 1341 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Neurons were cultured DIV 0 to DIV 4 in Neurocult™ Neuronal Plating Medium (Stemcell Technologies, catalog no. 05713) with SM1 neuronal supplement (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... hNPCs were grown on Matrigel-coated culture plates in Neural Progenitor Medium 2 (StemCell Technologies) up to 95-100% confluency ...
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bioRxiv - Cancer Biology 2021Quote: ... Hu Recom IL-2 (CHO Expr.) were purchased from Stem Cell Technologies (Vancouver, BC, Canada). The ATPLite Luminescence Assay System and 96-well white ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL Rho-kinase inhibitor (RockI; Y-27632 STEMCELL Technologies, cat. 72304). To prepare the differentiation experiments ...
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bioRxiv - Bioengineering 2023Quote: ... media was replaced with 2 mL Hematopoietic Media A (STEMdiff Hematopoietic Basal Media (StemCell Technologies) with STEMdiff Hematopoietic Supplement A (200x ...
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bioRxiv - Neuroscience 2023Quote: ... that was coated with 2 ml of anti-adherence rinsing solution (Stem cell technologies: 07010) for 5 mins before plating the cells ...
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bioRxiv - Developmental Biology 2021Quote: ... YS suspension or sorted cells were plated in triplicate (3×103 or 100-150 cells/mL) in Methocult® M3234 (StemCell Technologies) always supplemented the cytokines listed in supplemental table 5 ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IL-15 and incubated for 3 days before actively infected cells (Thy1.2+) were isolated by magnetic positive selection (Stem Cell Technologies; 18951). Infected cells (Thy1.2+ ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
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bioRxiv - Neuroscience 2023Quote: ... Daily media changes of E7™ Reprogramming Media followed until the emergence of iPSC colonies between day 21-28 when iPSC colonies were picked and transferred to Geltrex™ coated 12-well plates containing 1 mL TeSR™-E8™ (STEMCELL Technologies) supplemented with 10 μM ROCK Inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... at 5% CO2/95% air condition at 37°C and were passaged using ReLeSR™ (Stem Cell Technologies). Karyotype analysis of G-banded metaphase chromosomes has been performed to confirm chromosomal integrity ...
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bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/ml bFGF and 5 ng/ml BMP4 and plated in ultra-low attachment plates (Stem Cell Technologies). Two days later ...
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bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...
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bioRxiv - Genomics 2021Quote: ... Cells were split every 3 or 4 days with TrypLE Select (Fisher) and seeded at 1.5-4×10e5 in a Matrigel-coated p35 plate containing 5μM Y27632 (Stem Cell Technologies) (mTeSR+Y ...
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... for 4 days to induce iMSCs before the medium was changed to Complete MesenCult™-ACF Plus Medium (STEMCELL Technologies) for an additional 2 days and then the cells were transferred into a new 24-well plate without an MEF feeder layer ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Dual SMAD inhibitors (2μM Dorsomorphin; StemCell Tech; #72102, and 2 μM A83-01; StemCell Tech; #72022) until day 5 ...
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bioRxiv - Neuroscience 2021Quote: ... with the addition of the following supplements 2% (v/v) NeuroCultTM SM1 Neuronal Supplement (StemCell Technologies), 1% (v/v ...
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bioRxiv - Genetics 2022Quote: ... neuronal differentiation media was switched after 2 weeks to BrainPhys™ Neuronal Medium (Stem Cell Technologies) supplemented with the same factors to promote optimal neuronal activity and maturation.
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bioRxiv - Developmental Biology 2022Quote: ... Half well equivalent was seeded into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Neuroscience 2022Quote: ... were maintained in serum-free STEMdiff™ Neural Progenitor Medium 2 (Stem Cell Tech cat#08560) on Corning® Matrigel® hESC-qualified Matrix (Corning cat#354277 ...
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bioRxiv - Cell Biology 2020Quote: ... C3H10T1/2 cells and MSCs were treated with Mesencult ™ Osteogenic Stimulatory Kit (STEMCELL TECHNOLOGIES, #05504).
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bioRxiv - Developmental Biology 2023Quote: ... with 6 μM CHIR99021 in 1.2 mL STEMdiff™ APEL™2 medium (STEMCELL Technologies, # 05270) at the bottom of the chamber for 1-hour ...
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bioRxiv - Genetics 2022Quote: ... HUDEP-2 cells were expanded in a basal medium composed of StemSpan SFEM (Stem Cell Technologies) supplemented with 10−6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day the medium was replaced with STEMdiff™ APEL™2 (StemCell Technologies, #05270), containing 8 μM CHIR-99021 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... medium was changed to 2 mL of complete STEMdiffTM Astrocyte Differentiation Medium (ADM) (Stemcell Technologies, #08540). On day7 and day 14 ...
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bioRxiv - Cell Biology 2019Quote: ... Differentiation was initiated by changing to RPMI1640 medium containing B-27 (minus insulin) and the glycogen synthase kinase-3 (GSK3) inhibitor CHIR99021 (STEMCELL Technologies, cat. 72052) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids still inside the BME were washed 3 times with cold HBSS followed by a 20 min incubation with Dispase solution (StemCell Technologies, Cambridge, UK) at 37°C and 5% v/v CO2 to remove the BME around the tubuloids ...
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bioRxiv - Cancer Biology 2022Quote: ... endothelial and haematopoietic lineages in a 3-stage process as described in Figure 1A and the main manuscript text using APEL2 (StemCell Technologies Cat#05275)(61 ...
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bioRxiv - Microbiology 2021Quote: ... A nasal brushing of the turbinate was acquired using 3-mm bronchial cytology brush and placing the biopsy into warm PneumaCult-Ex Plus Medium (STEMCELL Technologies, Cambridge, UK). The cells were dissociated from the brush by gentle agitation before seeding into a single well of a collagen (PureCol from Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting ...
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bioRxiv - Cell Biology 2023Quote: ... 3.3 x 105 cells and 2.6 x 105 cells per ml were seeded in 3 ml E8 medium supplemented with 10 µM Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (RI, STEMCELL Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Immunology 2021Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×10^6 T cells/ml in serum-free StemCell Immunocult™-XF media (StemCell Technologies, Vancouver, Canada) with clinical grade 20U/ml human IL-2 (Novartis)