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Citations for Stemcell Technologies :
1101 - 1150 of 4243 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were enriched using the EasySep Human CD4+ T Cell Enrichment Kit (STEMCELL Technologies, Cat. No. 19052) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Differentiation of iPSCs to hematopoietic progenitor cells (HPCs) was done using Stem Cell Technologies STEMdiff Hematopoietic Kit (StemCell Technologies). Upon reaching 80 % confluency ...
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bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 50,000 cells/cm2 and passaged at 90% confluency by AccutaseTM (Stem Cell Tech cat#07920) dissociation as necessary ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Genetics 2023Quote: ... cells were plated into stem cell media without penicillin-streptomycin supplemented with CloneR2 (STEMCELL Technologies; cat. no. 100-0691) and 10 μM ROCK inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... at a density of 30 x 104 cells/well and cultured in NeuroCult neuronal plating medium (Stem Cell Technologies) supplemented with B-27 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
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bioRxiv - Immunology 2023Quote: NK Cells were enriched from freshly isolated PBMC using the EasySep™ Human NK Cell Isolation Kit (STEMCELL Technologies). NK cells were then counted and resuspended at 1M/mL in RPMI-1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining pellet was gently resuspended in enteric glial cell media supplemented with 1x CloneR (Stem Cell Technologies, 5889) and 50ug/mL primocin (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged twice a week after reaching approximately 80% confluency using PBS/EDTA or ReLeSRTM (Stem Cell Technologies) as a dissociation reagent ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from naïve splenocytes using the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies) and plated at 3x105 cells per well in a flat bottom 96 well plate ...
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bioRxiv - Biophysics 2023Quote: ... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
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bioRxiv - Bioengineering 2023Quote: ... Human pan T cells were purified from frozen peripheral blood mononuclear cells (PBMCs, STEMCELL Technologies, Cat #70025.2. Lot 201671701C) using a negative selection human Pan T Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... were seeded (70,000 cells each) in medium for long-term culture of human cells (catalogue no. 05150; STEMCELL Technologies) supplemented with hydrocortisone in collagen-coated plates ...
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bioRxiv - Immunology 2023Quote: ... B cells were extracted by negative selection with the EasySep™ Human B Cell Isolation Kit (StemCell Technologies, #17954). EBV-containing supernatants from B95-8 cells were added to purified B cells supported by 30 µg mL-1 holo-transferrin and 2.5 µg mL-1 CpG (ODN2006) ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in the presence of TGFβ and BMP inhibitors (LDN193189, #72142, Stem Cell Technologies; SB431542, #1614, Tocris) to trigger cortical precursors for cortical neuron differentiation ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were cultured in collagen-I gels in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, Cat# 10981) with the addition of Human Recombinant Interleukin 2 (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... The OSCs were produced in multiple batches and cryopreserved in vials of 120,000 to 150,000 live cells and stored in liquid nitrogen in CryoStor CS10 Cell Freezing Medium (StemCell Technologies). For the purposes of this study ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ T cells were activated on-chip by adding ImmunoCult™ Human CD3/CD28 T Cell Activator (StemCell Technologies) to the culture media ...
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bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated by magnetic sorting (EasySep™ Mouse CD8+ T Cell Isolation Kit, StemCell Technologies), followed by tetramer E7 and TRM marker staining ...
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bioRxiv - Developmental Biology 2023Quote: ... For stem cell maintenance N2B27 medium was supplemented with 3 μM CHIR99021 (Chir) (Catalog no. 72054, Stem Cell Technologies), 1 μM PD0305901 (Catalog no ...
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bioRxiv - Immunology 2023Quote: ... CVF was enriched for CD45+ cells using the EasySep™ Release Human CD45 Positive Selection Kit (Stem Cell Technologies) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using EasySep Human T Cell Isolation Kit (StemCell Technologies, Cat# 17951, lOT #1000153615). CD3+ T-cells were thawed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The OSCs were produced in multiple batches and cryopreserved in vials of 120,000-150,000 live cells and stored in liquid nitrogen in CryoStor CS10 Cell Freezing Medium (StemCell Technologies). The morning of oocyte retrieval ...
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bioRxiv - Immunology 2023Quote: ... dendritic cells (DCs) were enriched by magnetic isolation using the EasySepTM Mouse Pan-DC Enrichment Kit (Stem Cell Technologies). DCs were plated into 96-well U-bottom plates (100 × 106 cells/well) ...
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bioRxiv - Cancer Biology 2024Quote: 500 transduced MDA-MB-436 cells were mixed with 1 ml of methylcellulose (MethoCult H4034 Optimum, Stem Cell Technologies) in a 6-well cell culture plate and cultured at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Bioengineering 2024Quote: ... 600000 single stem cells were seeded per well (500 cells/microwell) of AggreWell™400 (STEMCELL Technologies, Vancouver, Canada) plates in aggregation media with 50 μM Y27632 (STEMCELL Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... iPSCs were washed 1x in PBS and dissociated into single-cell suspensions using Gentle Cell Dissociation Reagent (Stemcell Technologies) for 10 min at 37° C and seeded on to Geltrex-coated 12-well plates at a density of 700,000 cells in neural induction medium + 10 uM Y-27632 (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... CD3 T cells were isolated by negative selection following manufacturer’s protocol (RosetteSep Human T cell enrichment cocktail, STEMCELL Technologies). Following isolation ...
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bioRxiv - Immunology 2024Quote: ... Effector CD4+ T cells were isolated by magnetic enrichment using EasySep mouse CD4+ T cell Isolation Kit (STEMCELL Technologies) with addition of 10 µL of biotinylated anti-CD62L (BioLegend ...
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bioRxiv - Genomics 2024Quote: ... mouse NIH-3T3 cells were washed once with PBS and dissociated with Accutase (Stem Cell Technologies, catalog no. 07920). Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated from the samples using RosetteSep Human B cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... T cells were obtained from whole blood using the RosetteSep™ Human T Cell Enrichment Cocktail (StemCell Technologies, 15061), followed by density gradient separation using Ficoll® Paque Plus (Cytiva Life Sciences ...
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bioRxiv - Immunology 2023Quote: iPSc cell lines were furnished by the Nantes iPSc platform and were maintained on Matrigel (Stem Cell Technologies, 354277) coated 6-well plates in mTESR1 medium (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 2% FBS and 1.5ug/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies). Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70 ...
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bioRxiv - Immunology 2023Quote: ... Basophils were purified to >99% using negative selection antibodies and beads (STEMCELL Technologies, Cambridge, MA, USA).22 The pDC were prepared from the BDC suspension using BDCA-4+ selection after conducting 2 cycles of CD14+ selection to first remove monocytes.23
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bioRxiv - Cancer Biology 2021Quote: ... naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) and stimulated with plate-bound α-CD3 (5 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: Naïve (CD44loCD62Lhi) CD8+ T cells were purified using the EasySep™ Mouse Naïve CD8+ T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Peripheral blood mononuclear cells were cultured for 8 days in erythroblast expansion medium (StemSpan™ SFEM medium (Stem Cell Technologies) supplemented with Epo (2 U/mL) ...
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bioRxiv - Immunology 2021Quote: ... NK cell isolation was done by using the negative selection kit “RosetteSep™ Human NK Cell Enrichment Cocktail” (STEMCELL Technologies) according to the company’s protocol ...
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using SepMate-50 Isolation tubes (Stem Cell Technologies, Vancouver, Canada) per the manufacturer’s protocol ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... and T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were grown on either Corning or Eppendorf tissue culture-treated plates coated with Vitronectin XF (Stem Cell Technologies #7180) and passaged using either TrypLE Express Enzyme (Gibco ...