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Citations for Stemcell Technologies :
1051 - 1100 of 2099 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... media was switched to ‘ACL’ (100ng/ml Activin-A, Qk001, QKINE, 3μM CHIR99021, 72052, STEMCELL Technologies, and 10ng/ml human LIF) for hypoblast induction or ‘PA’ (1μM PD0325901 and 1μM A83-01 ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC from a previously characterized healthy control (line 2135 or C3 in 110) were cultured in mTeSR1 media (Stemcell Technologies) on vitronectin coated dishes ...
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bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
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bioRxiv - Bioengineering 2023Quote: ... ∼80% confluent human induced pluripotent stem cells (hPSCs) were dissociated into single cells with the Gentle Cell Dissociation Reagent (STEMCELL Technologies). Bioink consisting of 1.5mg/mL collagen with 5% Geltrex and high-density hPSCs (4×107 cells/mL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... The t(8;21)-positive patient primary cells were co-cultured on MSCs feeders and cultivated with SFEM II containing 1X Human Myeloid Expansion Supplement II (StemCell Technologies).
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Immunology 2022Quote: Monocytes isolated from BCs were obtained first by enrichment of the BC with 15µL of RosetteSep human monocyte enrichment cocktail (Stemcell Technologies, Sweden) per mL of BC ...
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bioRxiv - Immunology 2022Quote: ... 12 day-expanded cells were further expanded for 7 days with ImmunoCult Human CD3/CD28/CD2 T Cell Activator (25 μl/ml; STEMCELL Technologies), then rested overnight with reduced IL-2 as above (total 20-day culture) ...
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bioRxiv - Genomics 2022Quote: ... 1000 cells of each sorted population were seeded in 1 mL of methylcellulose medium supplemented with recombinant murine SCF and IL-3 with recombinant human IL-6 and Epo (M3434, STEMCELL Technologies) in duplicate ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Bioengineering 2024Quote: Isolated B cells were plated in ImmunoCult-XF B Cell Base medium supplemented with Human B Cell Expansion Supplement (StemCell Technology), seeding at 2.5 x 105 cells/mL in an appropriate format to provide media at a depth of 2-5 mm ...
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bioRxiv - Cancer Biology 2024Quote: Purified HSPCs were plated in 96 well plates containing megakaryocyte differentiation medium (StemSpan SFEM II with human low-density lipoproteins (STEMCELL Technologies) and Megakaryocyte Expansion Supplement (STEMCELL Technologies)) ...
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bioRxiv - Cell Biology 2024Quote: ... In some experiments we also used an additional commercially available ENR medium (here denoted cENR; IntestiCult Human organoid differentiation medium; #100-0214, STEMCELL Technologies) with 100U/ml penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then stimulated with 12 μL/mL ImmunoCult™(TM) Human CD3/CD28 T Cell Activator (STEMCELL Technologies, cat. #10971).
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bioRxiv - Microbiology 2024Quote: ... We initiated the organoid development by plating hiPSCs at density 20- 30% in TeSR1 medium on a 6-well plate coated with human embryonic stem cell (hESC)-qualified basement membrane matrix (STEMCELL Technologies). The next day ...
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bioRxiv - Neuroscience 2024Quote: Human induced pluripotent stem cell (hiPSCs) were obtained from WiCell (IMR90-4/WISCi004, WiCell) and cultured in mTeSR+ medium (STEMCELL Technologies) supplemented with 1% (v/v ...
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bioRxiv - Immunology 2024Quote: ... Blood monocytes and T cells were enriched and obtained by counterflow centrifugal elutriation using RosetteSep human monocyte and T cell enrichment cocktail (StemCell Technologies), respectively ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from peripheral blood of healthy donors under a University of Wisconsin-Madison IRB-approved protocol (2017-1070) using the RosetteSep Human T Cell Enrichment Cocktail (cat. no. 15021, 15061; STEMCELL Technologies). The sequence for a third generation GD2-CD28-OX40-CD3ζ construct was generously provided by Malcolm Brenner (Baylor College of Medicine) ...
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bioRxiv - Immunology 2024Quote: ... at the final concentration of 0.3×106 cells/ml and stimulated with ImmunoCult™ Human CD2/CD3/CD28 T Cell Activator (Stemcell Technologies). In the next step ...
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bioRxiv - Neuroscience 2024Quote: ... the NPM was exchanged for lateral ganglionic eminence (LGE) pattering medium (N2B27 supplemented with 25 ng/mL recombinant human activin A) (STEMCELL Technologies). The medium was changed every day until day 10 when the LGE progenitors were passaged for terminal differentiation into MSNs ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purified from healthy donor blood samples using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies). For cloning ...
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bioRxiv - Immunology 2024Quote: Monocytes were isolated from healthy human donor buffy coat (Department of Transfusion & Tissue Medicine of the Brno University Hospital) using RosetteSepTM Human Monocyte Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: When human iPSCs reached a confluency of approximately 95% they were passaged with AccutaseTM Cell Dissociation Reagent (Cat #07920, StemCell Technologies) and resuspended as single cells in mTesRTM1 medium with 10 µM Y-27632 (Tocris Bioscience) ...
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bioRxiv - Cancer Biology 2024Quote: ... Human peripheral blood mononuclear cells from healthy donors were thawed from cryostorage in ImmunoCult-XF medium (Stem Cell Technologies # 100-0956) containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970 ...
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bioRxiv - Bioengineering 2024Quote: Patient-derived glioblastoma stem cell lines SD2 and SD3 19 were transduced with GFP-expressing lentivirus or were genetically engineered with a doxycycline-inducible H2B-GFP reporter 19 and cultured in laminin-coated tissue culture flasks in complete NeuroCult NS-A proliferation medium for human cells (Stemcell Technologies). U87MG cells (ATCC ...
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bioRxiv - Cancer Biology 2024Quote: Patient-derived colorectal cancer organoids were established and cultured in our Pathology Department using IntestiCult™ Organoid Growth Medium (Human) following the manufacturer’s protocol (Stemcell Technologies)18 ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF human iPSCs were seeded in a Matrigel-coated T25 flask in mTeSR1 medium (Stem Cell Technologies, #85870) with ROCK inhibitor ...
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bioRxiv - Bioengineering 2021Quote: ... CFU-E and CFU-GEMM Colony identification was performed 14 days after seeding in MethoCult media according to the Atlas of Human Hematopoietic Colonies (StemCell Technologies, 28700).
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coat (Research Blood Components) using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The crypts were embedded in 30 μl of Matrigel and cultured with Human IntestiCult™ Organoid Growth Medium (OGM) (Basal Medium and Organoid Supplement, Stemcell Technologies) supplemented with 100 IU Penicillin and 100 μg/ml Streptomycin (Corning ...
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bioRxiv - Cancer Biology 2022Quote: Differentiated human and murine H3K27M glioma cells were generated by dissociating the respective gliomaspheres into single cells with Accutase (Stemcell Technologies; # 07920) and subsequently cultured and maintained in the respective base media supplemented with 10% FBS for 14 days to generate a monolayer adherent culture ...
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bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Immunology 2020Quote: ... Primary human airway epithelial cells isolated via bronchial brushings from consented healthy individuals were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from de-identified human leukapheresis-processed blood (New York Biologics, Inc) by centrifugation through Lymphoprep (Stem Cell Technologies.) density gradient ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: Culture of isolated human CB-derived CD34+ cells was performed in HSPC expansion media comprised of Stem Span serum-free media (StemCell Technologies #09855) supplemented with Glutamax (Invitrogen #35050061) ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from de-identified human leukapheresis-processed blood (New York Biologics, Inc) by centrifugation through Lymphoprep (Stem Cell Technologies.) density gradient ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Developmental Biology 2023Quote: CD34+ cells were enriched from de novo progenitor cells collected from each respective 3D hydrogel tissue explant culture using the EasySep Human CD34 Selection (StemCell Technologies, #17856). HUCB CD34+ cells were isolated from whole blood using Lymphoprep (StemCell Technologies ...