Labshake search
Citations for Stemcell Technologies :
1051 - 1100 of 1660 citations for Glucagon like peptide 1 receptor GLP1R Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
-
bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
-
bioRxiv - Immunology 2021Quote: Untouched total T or isolated CD4+ or CD8+ T cells were purified from buffy coats or whole blood with RosetteSep Human T or CD4+ or CD8+ T cell enrichment cocktails (STEMCELL Technologies, Cat#: 15061, 15062, 15063). Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... the remaining fresh murine BM cells were purified using human CD45 MicroBeads (Miltenyi Biotech) and cryopreserved in CryoStor® CS5 (STEMCELL Technologies, Cambridge, MA, USA). For secondary transplantation ...
-
bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: The first step consists in a CTC enrichment from whole blood sample by immunomagnetic negative selection using the EasySep™ Direct Human CTC Enrichment Kit (StemCell Technologies®, Vancouver, Canada) according to manufacturer instruction ...
-
bioRxiv - Cell Biology 2024Quote: Primary human T cells were isolated from the peripheral blood of a healthy donor using a negative selection kit (EasySep, STEMCELL Technologies Canada Inc., Vancouver, BC) and used for testing the sensitivity of the lifetime flow system to T cell activation ...
-
bioRxiv - Bioengineering 2024Quote: ... PBMC samples were thawed on the day of activation and T cells were purified via the EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies Inc, cat#19053). Isolated T cells were seeded in the CD3/CD28 precoated well plates at a concentration of 500,000 cells/mL with 1 mL/well in ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies Inc ...
-
bioRxiv - Physiology 2020Quote: ... were then cultured in the complete L15 medium or the stem cell (SC) medium (10% human MammoCult Proliferation Supplements in MammoCult Basal Medium, Stem Cell Technologies, San Diego, CA, USA) for 7 days ...
-
bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
-
bioRxiv - Immunology 2022Quote: ... CD8+ T-cells were negatively isolated from PBMC by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies; average purity 93%, n=16).
-
bioRxiv - Cancer Biology 2023Quote: ... T cells were then isolated using EasySep™ Human T Cell Isolation Kit and EasySep™ Magnet (Stemcell Technologies, Cat. No. 17951 and Cat. No. 18000) following manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... with 1% PS and 1 μg/mL doxycycline (StemCell Technologies 72742). Prior to orthotopic injection of primary PDAC cell lines ...
-
bioRxiv - Microbiology 2024Quote: ... a 1:1 mixture of organoid supplement (Stemcell technologies, 100-0191) and Intesicult™ OGM Human Basal Medium (Stemcell technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... domes diluted 1:1 with advanced DMEM/F12 media (Stemcell Technologies, UK), with 50 μl domes per well ...
-
bioRxiv - Physiology 2022Quote: ... then medium changed to 1:1 cFAD+RI:Pneumacult ALI (Stemcell Technologies, UK) for 2 days to ensure monolayers were 100% confluent ...
-
bioRxiv - Cell Biology 2021Quote: Hepatostellate organoids at day 17 were treated with dissociation buffer, (1:1:1 mixture of 0.25% Trypsin-EDTA, Accutase (Stem cell Tech.), and Collagenase IV (1 mg/mL ...
-
bioRxiv - Immunology 2022Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
-
bioRxiv - Developmental Biology 2022Quote: ... medium was replaced by 1 ml of a 1:1 mixture of UPC medium and reprogramming medium TeSR-E7 (STEMCELL Technologies). From day 4 to day 18-20 ...
-
bioRxiv - Immunology 2021Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
-
bioRxiv - Immunology 2023Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin (R1) and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
-
bioRxiv - Microbiology 2023Quote: ... mixed 1:1 with PneumaCult™-Ex Plus Medium (STEMCELL Technologies cat# 16237380). All cell lines were discarded after passage 15 ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1:10 dilution of 1 mg/ml DNase I (STEMCELL Technologies), triturated vigorously for 2 min ...
-
bioRxiv - Neuroscience 2021Quote: ... These cells were cultured in mTeSR™1 medium (mTeSR™1 Basal Medium with mTeSR™1 5× Supplement (Stemcell Technologies) and 1% penicillin-streptomycin (P/S ...
-
bioRxiv - Immunology 2020Quote: ... Remaining whole blood was diluted 1:1 with 1% RPMI (Table S4) and layered into a SEPMATE tube (STEMCELL Technologies, Cat#85450) pre-loaded with lymphoprep (Alere Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
-
bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... the media was changed to pre-warmed 37°C mesoderm / Endothelial “priming” media (8 uM CHIR99021 +25 ng/ml BMP4, 1× Glutamax, 1× N2, 1× B27, Neurobasal, DMEM/F12, Stem Cell Technologies). One well of seeded cells was left untreated as negative control in StemFlex (data not shown) ...
-
bioRxiv - Cancer Biology 2022Quote: ... all the unwanted cells were labelled with magnet conjugated antibody cocktail which is separated using their EasySep magnetic stand leaving CD4 or CD8 cell in suspension using the vendor supplied EasySep Human CD4/CD8 T Cell Iso Kit (Stem Cell Technologies, 17952 for CD4 and 17953 for CD8). This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals ...
-
bioRxiv - Developmental Biology 2021Quote: ... Purmorphamine (1 µM, STEMCELL Technologies), a small molecule agonist of the hedgehog pathway ...
-
bioRxiv - Genomics 2020Quote: ... 1 µM PD0325901 (Stemcell Technologies), 0.033% BSA solution (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μM hydrocortisone (STEMCELL Technologies), and 200 μg/ml Primocin ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 × NeuroCult SM1 (StemCell Technologies), and 1 × Antibiotic-Antimycotic ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 μM hydrocortisone (StemCell Technologies), 20 ng/ml of IL-3 ...
-
bioRxiv - Microbiology 2020Quote: ... 1% supplement (Stemcell Technologies, 05731), 0.5% N2 supplement (Gibco ...
-
bioRxiv - Molecular Biology 2022Quote: ... with mTeSR-1 (STEMCELL Technologies). Lines used in this manuscript include ...
-
bioRxiv - Neuroscience 2023Quote: ... 1% N2 Supplement (Stemcell Technologies), 1% GlutaMAX (Life Technologies)
-
bioRxiv - Neuroscience 2023Quote: ... DMH-1 (73634, StemCell Technologies), SB-431542 (72234 ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% N2 (07152, STEMCell Technologies), and 1% GlutaMax (Gibco) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µM CHIR99021 (Stemcell Technologies) and placed in a CelVivo Clinostar incubator (Invitro Technologies ...
-
bioRxiv - Immunology 2024Quote: ... + 1 µM BIRB796 (Stemcell Technologies) were added to the culture medium ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 µM UM729 (Stemcell Technologies), 750nM SR1 (Stemcell Technologies) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 100nM IGF-1 (StemCell Technologies), 1x N2 Supplement (Thermofisher Scientific) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1 μM PD0325901 (STEMCELL Technologies)) and passaged as single cells onto fresh feeders using TrypLE Express (Gibco) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1%L-glutamine (STEMCELL Technologies), heparin 0.1 mg/ml (Sigma-Aldrich) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 µM CHIR99021 (StemCell Technologies), 2 µM SB431542 (StemCell Technologies) ...
-
bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
-
bioRxiv - Genomics 2020Quote: ... Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...
-
bioRxiv - Genomics 2021Quote: ... at a 1:100 dilution and in mTeSR™1 Medium (85851, STEMCELL Technologies, Vancouver, Canada) supplemented with 1% Penicillin/Streptomycin (30-002-CI ...
-
bioRxiv - Genomics 2021Quote: Tissues were minced into ~1-2 mm pieces and digested with 1× Collagenase/Hyaluronidase (STEMCELL Technologies) and 100 μg/mL DNase I (Sigma-Aldrich ...