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Citations for Stemcell Technologies :
1001 - 1050 of 1749 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies) for definitive endoderm induction ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies). Media was replaced with RPMI 1640 medium ...
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bioRxiv - Neuroscience 2024Quote: ... hESCs were fed every two days and split every 4–7 days using ReLeSR (StemCell Technologies, 100-0483) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μM IWR-1-endo (StemCell Technologies, 72562), 5 μM SB-431542 (StemCell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: CFC assays from ES and iPS cells from EB or BL-CFC were performed by plating 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies) in triplicate ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP positive cells with a moderate GFP signal were seeded to Matrigel-coated 96-wells in mTeSR Plus medium supplemented for 3 days with CloneR (both StemCell Technologies). After 3 days ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: CFC assays from ES and iPS cells from EB or BL-CFC were plated at 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies). Hematopoietic CFC was counted at day 19.
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bioRxiv - Immunology 2024Quote: ... 0.6 μg of a rat anti-mouse F4/80-PE antibody was incubated for 15 min at room temperature and samples were washed with 3 mL of EasySepTM buffer (ESB) (cat. no. 20144, STEMCELL Technologies), centrifuged ...
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bioRxiv - Cell Biology 2024Quote: ... IL-3/IL-6 and SCF cultures:3 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... CD34+ cells were plated at a density of approximately 25,000-100,000 cells per well and spun down at 250 RPM for 3 minutes in StemSpan SFEM II media (STEMCELL Technologies, 09655). Pre-warmed media was used to resuspend the CD34+ cells ...
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bioRxiv - Immunology 2024Quote: ... splenic murine T cells were freshly isolated from C57BL/6 mice using the EasySep™ Mouse T Cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada; Cat. No. 19851) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... NPCM is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 without retinoic acid (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... and maintained in culture medium containing mTeSR plus basal medium and 5 x supplement (Stem Cell Technologies). Thiazovivin (2 μM ...
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bioRxiv - Neuroscience 2021Quote: ... Cultures were fed every other day and passaged every 5–7 days by ReLeSR™ (STEMCELL Technologies).
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bioRxiv - Bioengineering 2023Quote: ... The iPSCs were routinely passaged every 5-7 days using ReLeSR™ Passaging Reagent (Stemcell Technologies, Canada). The iPSCs were then differentiated into SCPCs based on a modified protocol to generate spinal motor neurons (Hor et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The EBs were cultured in the plate for 5 days with neural induction medium (Stem cell technologies) to initiate neural induction ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... transduced AML cells were cultured on MS-5 cells (DSMZ) in Myelocult media H5100 (StemCell Technologies Inc.) with rhIL-6 ...
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bioRxiv - Immunology 2024Quote: ... In short, PBMCs were pelleted (300g, 5 min) and resuspended in EasySep™ Buffer (20144; Stemcell Technologies) at a concentration of 1 x 108 cells/mL and incubated with 100 µL selection antibody cocktail per mL of sample for 10 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... the medium was replaced with RPMI 1640 containing 5 μM XAV-939 (Stem Cell Technologies, Cat.#72674), 500 μg/mL BSA ...
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bioRxiv - Cell Biology 2024Quote: ... for the first day after passages and subcultured every 5–7 days using ReLeSR reagent (STEMCELL Technologies). The pluripotency of iPS cells were characterized by immunostaining with OCT3/4 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were cultured DIV 0 to DIV 4 in Neurocult™ Neuronal Plating Medium (Stemcell Technologies, catalog no. 05713) with SM1 neuronal supplement (Stemcell Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 µM DMH-1 (STEMCELL Technologies, cat# 73632)13 ...
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bioRxiv - Genomics 2021Quote: ... diluted 1:40 in mTeSR-1 medium (StemCell Technologies). hiPSCs were passaged upon treatment with Accutase (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mg/mL DNase 1 (Stem Cell Technologies). The samples were digested for 1 hour at 37°C with constant low agitation using a thermomixer (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... Tra-1-60 (#60064, STEMCell Technologies at 1:200), stage-specific embryonic antigen-4 (SSEA-4 ...
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bioRxiv - Molecular Biology 2023Quote: ... NeuroDiff medium is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... YS suspension or sorted cells were plated in triplicate (3×103 or 100-150 cells/mL) in Methocult® M3234 (StemCell Technologies) always supplemented the cytokines listed in supplemental table 5 ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IL-15 and incubated for 3 days before actively infected cells (Thy1.2+) were isolated by magnetic positive selection (Stem Cell Technologies; 18951). Infected cells (Thy1.2+ ...
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bioRxiv - Neuroscience 2021Quote: ... at 5% CO2/95% air condition at 37°C and were passaged using ReLeSR™ (Stem Cell Technologies). Karyotype analysis of G-banded metaphase chromosomes has been performed to confirm chromosomal integrity ...
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bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
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bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/ml bFGF and 5 ng/ml BMP4 and plated in ultra-low attachment plates (Stem Cell Technologies). Two days later ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... iPSCs were maintained at 37 °C and 5% CO2 in mTeSR or mTeSR plus medium (Stem Cell Technologies) on Geltrex-coated (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Purified human CB CD34+ HSPC were isolated and cultured at 5×105/mL in StemSpan SFEMII (Stemcell Technologies) supplemented with human stem cell factor (150ng/mL) ...