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Citations for Stemcell Technologies :
1001 - 1050 of 1155 citations for 2E 4E 2 4 Octadien 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: HSPCs were plated at a concentration of 1 × 103 cells/ml in a methylcellulose-containing medium (GFH4435, STEMCELL Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Systems Biology 2023Quote: ... TRA-1-60) as well as differentiation into the three germ layers (STEMdiff Trilineage Differentiation kit, #05230, StemCell Technologies).
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended at a concentration of 1×106 cells/mL in neuron media [Brainphys (StemCell Technologies #05790), 1×N2 (ThermoFisher #17502-048) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD11B-FITC (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Cell Biology 2023Quote: ... 1% (v/v) B27™ minus insulin) supplemented with 5 µM CHIR99021 and 33.3 ng Activin A (STEMCELL Technologies). Medium was exchanged daily without CHIR99021 supplementation ...
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bioRxiv - Neuroscience 2024Quote: ... the plating medium was removed and replaced by Neurobasal medium supplemented with SM1 supplement (StemCell Technologies; 1:50 dilution), 0.5 mM glutamine and 0.12 mg/mL gentamycin ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were seeded at a density of 1.6-5.8 × 104 cells/cm2 on plates coated with a 1:30 dilution of Matrigel in mTeSR+ (STEMCELL Technologies) supplemented with 100 U/mL penicillin-streptomycin and 5 µM ROCK inhibitor Y-27632 ...
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bioRxiv - Immunology 2021Quote: ... inguinal lymph nodes from immunized or naïve mice or live LN sections were incubated in 1 mL of digestion solution comprised of RPMI1640 medium containing 0.8 U/mL Dispase (07913, Stemcell Technologies), 0.1 mg/mL Collagenase D (11088858001 ...
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bioRxiv - Cell Biology 2021Quote: ... Rescue of epithelial integrity was induced by threating hiPSCs for 1 day with LPA (StemCell Technologies, ref. 72694, 5 µM). Chemical disruption of epithelial integrity was induced by threating hiPSCs for 1 day with Blebbistatin (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... The yolk sac embryoid bodies (YS-EBs) were generated by treating the YS-EBs with mTeSR 1 media (STEMCELL Technologies) supplemented with bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Cancer Biology 2021Quote: CD34+ cells isolated from MF patients were plated at a density of 500 cells/replicate in 30-mm dishes containing 1 mL of StemSpan SFEM with 1.1% methylcellulose (Cat# H4230, StemCell Technologies), to which SCF ...
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bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Biophysics 2020Quote: ... The cells were resuspended in 1 mL of medium contained 1x Stem Span Cytokine Cocktail CC100 (Stem Cell Technologies, Canada) in 24-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... derived from different mice using a previously described method23) and selected for genomic integration using puromycin (2.5 μg mL-1; StemCell Technologies) using established procedures28 ...
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bioRxiv - Genomics 2020Quote: NSTcPA buffer (1 mL) was pipetted into one well of a 6-well plate (Stem Cell Technologies, cat. no. 38015) on ice ...
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bioRxiv - Immunology 2022Quote: ... and cells from wells with reactive supernatants were expanded to 48-well plates for 1 week in 0.5 mL of StemCell medium E (StemCell Technologies), before being screened again by ELISA ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies) and cultured in NbM supplemented with 0.1 µM Retinoic Acid (RA ...
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bioRxiv - Neuroscience 2024Quote: ... The yolk sac embryoid bodies (YS-EBs) were generated by treating the YS-EBs with mTeSR 1 media (STEMCELL Technologies) supplemented with bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 of sorted lentivirally transduced CD34+ HSPCs were seeded into 1 mL of MethoCultTM H4434 Classic media (STEMCELL Technologies #04434) supplemented with StemSpan™ CC100 with antibiotics ...
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bioRxiv - Immunology 2024Quote: ... Freshly harvested organ tissue from male and female bats was washed in PBS and cut into 3-5 mm small pieces before placing up to four individual pieces into a 1 ml cryostorage tube containing 0.5ml-1ml of Cryostore10 freezing medium (Stemcell Technologies). Vials were immediately transferred to a cryostorage container (Mr ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD34-PE (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... FITC-conjugated mouse IgG2b anti-human CD32 Ab (clone IV.3, cat. no. 60012Fl.1, StemCell Technologies, Vancouver, BC, Canada). Mouse isotype controls APC-conjugated mouse IgG2a ...
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bioRxiv - Cell Biology 2024Quote: ... CS-001) were cultured on Matrigel-coated plates in feeder-free conditions in mTeSR-1 medium (Stemcell Technologies, Vancouver, Canada). As schematized in Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... or 1 mM db-cAMP (from 500 mM stock in 50/50 in Ethanol/PBS) (STEMCELL Technologies, Vancouver, BC, Canada), 0.5 mM 3-Isobutyl-1-methylxanthine ...
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bioRxiv - Cancer Biology 2024Quote: ... on Vitronectin (GibcoTM Cat#A14700, VTN-N) coated dishes and in mTESR1 (Stem Cell Technologies Cat#85850, mTeSR™1) on Matrigel (Corning Cat#354277 ...
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bioRxiv - Cancer Biology 2021Quote: ... Patient sample mononuclear cells were cultured in RPMI with 10% FBS and 50% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 uM UM729 (StemCell Technologies).
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bioRxiv - Genomics 2021Quote: ... they were seeded at approximately 30% confluency in Matrigel-coated culture dishes and cultured in mTeSR™1 (85851, STEMCELL Technologies) until cells fully adhered to the plate (at least 3 hours) ...
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bioRxiv - Genomics 2020Quote: ... Lymph nodes were harvested from a 6-week-old GFP OT-1 mouse and CD8 T cells were isolated using an EasySep mouse CD8 T cell negative selection kit (StemCell Technologies). 14 days post tumor injection ...
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bioRxiv - Genomics 2020Quote: Unsorted floating cells were taken from d2-3 of the blast culture and 5×103 cells were seeded in 1 ml MethoCult (M3434 STEMCELL Technologies) per dish ...
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bioRxiv - Neuroscience 2020Quote: ... carrying genes for tdTomato and the murine cell-surface protein CD90.2/Thy1.2 driven by the endogenous POU4F2 (BRN3B) promoter were clonally propagated in mTeSR-1 media (StemCell Technologies, Cambridge, MA) on growth factor-reduced Matrigel substrate (Corning ...
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bioRxiv - Cancer Biology 2020Quote: ... at 2.5 × 105 cells/well and topped off with 1 mL of either EpiCult™ Medium (StemCell Technologies, cat. no. 05610) supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Lin−Sca-1+c-Kit+CD150+CD48− (HSC) or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) populations were cultured in Myelocult M5300 (STEMCELL Technologies) with 100 µg/ml Primocin (Invivogen ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting single cell suspension was than washed with 10 mL of cold RPMI and resuspended in 1 mL of Robosep buffer (STEMCELL technologies) prior to the negative selection of B- or T-cells ...
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bioRxiv - Cancer Biology 2021Quote: ... and were plated in a concentration of 9,000 cells/well into an ultra-low-binding 96-well plate in mTeSR™1 medium (Stemcell technologies) containing Rho-associated protein kinase (ROCK ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation of the cells with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were allowed to recover in antibiotic-free medium containing 1 µg/mL DNase I solution (STEMCELL Technologies, Cambridge, MA) at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... AP positive colonies were then manually picked and seeded for further expansion onto Matrigel-coated wells of 24-well plates with mTeSR-1 (STEMCELL Technologies) maintenance medium with 10 µM Y-27632 ROCK inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... Day 8 differentiated Ngn2 neurons were re-seeded at a density of 1×105 cells per 100 µL droplet in CM2 media consisting of BrainPhys (STEMCELL Technologies), 1x penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were re-plated in mTeSR1 medium supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific) and 10 mM Rho-associated protein kinase (ROCK) inhibitor (Stemolecule Y-27632, STEMCELL Technologies) for 24 hr ...
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bioRxiv - Neuroscience 2020Quote: ... when they reached high confluency and re-plated in mTeSR 1 with RHO/ROCK Pathway Inhibitor Y27632 (Stem Cell Technologies, 72302). Patient donors are identified with a code with one numeral (DEL_1 ...
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bioRxiv - Microbiology 2020Quote: ... ectoderm differentiation was performed with basal Medium (DMEM/F12, 10% knockout serum replacement (KOSR) and 1% Glutamax) supplemented with 10 μM SB431542 (STEMCELL Technologies) and 200 nM Noggin (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... larvae were tricaine-anesthetized and embedded within optically clear glass imaging cubes (Mizar Imaging) in embryo medium with 1% low melt agarose and 20% OptiPrep (Stemcell Technologies, Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... Medium was replaced every other day and cells were passaged ~1:10 onto fresh Matrigel-coated vessels using ReLeSR (StemCell Technologies) when 90% confluent.
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bioRxiv - Cancer Biology 2022Quote: ... Patient samples were cultured in RPMI with 10% FBS and 10% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 µM UM729 (StemCell Technologies).
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bioRxiv - Neuroscience 2024Quote: ... In vitro differentiation was achieved by sequential culture of the cells in the following media: Step 1 (mTeSR (Stem Cell Tech) + 80 ng/ml BMP4 (PeProtech ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...