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Citations for Stemcell Technologies :
951 - 1000 of 2070 citations for Human Endothelin 2 EDN2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Each plate was then split 1:2 using ReLeSR (StemCell Technologies), with half of the cells used for DNA extraction for Sanger sequencing ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... 2% BSA (Miltenyi) and 10 uM ROCK inhibitor (StemCell technologies 72302). For all wash steps ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Cancer Biology 2022Quote: ... DNTs were enriched by depleting CD4+ and CD8+ cells using RosetteSep™ human CD4- and CD8-depletion cocktails (Stemcell Technologies). The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3 ...
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bioRxiv - Microbiology 2022Quote: ... Monocytes were isolated from whole blood by negative selection using RosetteSep human monocyte enrichment cocktail following the manufacturer’s protocol (StemCell Technologies). Using density gradient medium (Lymphoprep ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... human iPSCs were seeded on a matrigel coated dish and cultured in the STEMdiff SMADi Neural Induction medium (STEMCELL Technologies) for 7 days34 ...
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bioRxiv - Cell Biology 2020Quote: For structured illumination microscopy primary NK cells were enriched from peripheral blood of healthy human donors using RosetteSep (Stemcell Technologies). Freshly isolated cells were pre-incubated with anti-CD56 Alexa Fluor 647 (clone HCD56 ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
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bioRxiv - Cell Biology 2022Quote: hematopoietic progenitors (CD34+CD43+ for iPSC and CD34+ for primary cells) were plated in human methylcellulose medium H4434 (STEMCELL technologies), containing recombinant human cytokines and scored for the presence of colonies 14 days after.
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in a 1:1 ratio of DMEM-F12 and NeuroCult NS-A Basal Medium (Human) (Stem Cell Technologies) with 1X N-2 supplement (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... HUCB CD34+ cells harvested from Matrigel Matrix were subsequently enriched using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). Enriched CD34+ were then either used for FACS ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Primary Human Bronchial Epithelial Cells (HBEC) were expanded and differentiated in ALI culture following the protocol given by StemCell Technologies. Briefly ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies # 15061) or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953 ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary NK cells were isolated from peripheral blood by negative selection using RosetteSep human NK enrichment cocktail (Stemcell Technologies, 15065). Blood was layered onto a Ficoll-Paque (Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from human donor leukopaks by gradient centrifugation on lymphoprep (cat#07861, Stemcell Technologies). Thereafter ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Cancer Biology 2024Quote: Freshly isolated human HSPCs were plated at 200,000 cells/ml and cultured in IMDM medium supplemented with 25% BIT9500 (StemCell Technologies), 1% penicillin/streptomycin (P/S) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Developmental Biology 2020Quote: ... We used the commercial kit (STEMdiff™ Cerebral Organoid Kit, StemCell Technologies 08570) with previously described modifications to increase embryoid body surface area75 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 million cells were passaged per each well using Accutase (StemCell Technologies) into the STEMdiff SMADi neural induction medium (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml basic-FGF and 2 μg/ml Heparin (StemCell Technologies). L0125 and L0512 GBM tumorspheres were kindly provided by Dr ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml basic-FGF and 2 μg/ml Heparin (StemCell Technologies). S24 Tdtomato/GFP cells were kindly gifted by Dr ...
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bioRxiv - Neuroscience 2022Quote: ... Heparin solution 2 μg/ml (cat# 07980, Stem Cell Technologies, Vancouver, Canada), EGF 20 ng/ml (Cat#315-09 ...
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bioRxiv - Molecular Biology 2023Quote: HUDEP-2 cells were grown in StemSpan SFEM (STEMCELL Technologies, Vancouver, Canada) supplemented with 50ng/mL stem cell factor ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µM (for iPSC) or 5 µM (for ESC) XAV939 (Stemcell Technologies), and 10 µM Y27632 ...
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bioRxiv - Molecular Biology 2023Quote: ... HUDEP 2 cells were cultured in Stemspan Media (SFEM II - STEMCELL Technologies) with 50ng/ml SCF (ImmunoTools) ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 for 2 weeks in supplemented mTeSR medium (STEMCELL Technologies #85850). For KO of C3orf58 or SLC39A9 in iPSC ...
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bioRxiv - Cancer Biology 2023Quote: ... and fed IL-2 (50 U/mL; Stem Cell Technologies, Vancouver, Canada) every 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged 1-2 times per week with ReLeSR (STEMCELL Technologies) when they reached 80-90% confluence ...
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bioRxiv - Cell Biology 2020Quote: ... To inhibit these pathways we used Recombinant Human Noggin Protein for BMP4 inhibition and/or XAV-939 for WNT inhibition (StemCell Technologies).
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bioRxiv - Cell Biology 2019Quote: ... Primary CD4+ T cells were purified from the blood of healthy donors by negative selection using the Human CD4+ T Cell Enrichment cocktail (StemCell Technologies). These primary cells were cultured in complete RPMI 1640 medium.
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bioRxiv - Neuroscience 2021Quote: ... integrated and inducible pure human cortical-like glutamatergic neurons (i3Ns) was completed in two weeks in BrainPhys media (STEMCELL Technologies, 05790) (Figure ...
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bioRxiv - Developmental Biology 2019Quote: Human iPSC line WTC11 (a kind gift from Bruce Conklin, Gladstone Institute of Cardiovascular Disease) was maintained in mTeSR1 (Stemcell Technologies) on Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were isolated from peripheral blood of donor 2395 via negative selection using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human CD4+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 and 15023). T cells were cryopreserved in RPMI-1640 (Corning #10-040-CV ...
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bioRxiv - Immunology 2021Quote: ... cells were preactivated for 5 days with IL-2 and either 1:1 aAPCs or 25 μl/ml CD3/CD28/CD2 tetramer (ImmunoCult Human CD3/CD28/CD2 T Cell Activator, STEMCELL Technologies). L cells (ATCC CRL-2648 ...
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bioRxiv - Bioengineering 2021Quote: ... and 25 ng/mL BMP4 with 50 ng/mL human recombinant Vascular Endothelial Growth Factor 165 (VEGF-165, STEMCELL Technologies, 78073) for 2 days ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... between 50-250*103 cells per 200µl of the following culture media: Human LT-HSCs (LIN−/CD38lo/CD34+/CD45RA−/CD90+) in StemSpan Serum-free expansion medium (SFEM; Stemcell Technologies) supplemented with 100ng/ml human SCF ...
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bioRxiv - Microbiology 2022Quote: ... HNAE cells were collected from healthy donors (human ethics approval GLY/01/15/HREC) and expanded using Pneumacult Ex+ (Stemcell technologies). HNAE cells were differentiated at air-liquid interface in 6.5 mm transwell with a 0.4μm polyester 156 membrane (Corning ...