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Citations for Stemcell Technologies :
951 - 1000 of 1036 citations for 7 Benzyl 4 chloro 5 6 7 8 tetrahydropyrido 3 4 d pyrimidin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Bioengineering 2023Quote: ... we washed 80-90% confluent cultures with PBS-and dissociated cultures into a single-cell suspension with 5 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Developmental Biology 2023Quote: ... Four-month-old cortical organoids were randomly selected and transferred to a recording chamber kept at 37L°C using a heating platform and a controller (TC-324C, Warner Instruments) in 5% methyl-cellulose in BrainPhys Imaging Optimized Medium (STEMCELL Technologies, #05796). Imaging was performed using a SP-8X microscope with a multiphoton laser ...
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were seeded at 70% confluency on dishes pre-coated with Matrigel and cultured in StemMACS iPS-Brew XF + 5 μM Y-27632 (STEMCELL Technologies 72304). On D1 ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were cultured at 1×105–5×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies, Vancouver, Canada) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell pellet was resuspended into HF buffer (HBSS, 2% fetal bovine serum, 10mM Hepes pH 7.2) and NH4Cl (StemCell Technologies). The cell pellet was recovered after 5 min of centrifugation at 1000rpm and resuspended in organoid medium for organoid culture ...
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bioRxiv - Neuroscience 2020Quote: ... Transduced hiPSCs were selected and expanded in the presence of 2 μM puromycin and cryo-stored in mFreSR (STEMCELL Technologies) or further differentiated to OPCs as described below.
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bioRxiv - Immunology 2019Quote: ... Isolated T cells were cryopreserved at 2×107 T cells per vial in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
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bioRxiv - Neuroscience 2022Quote: ... For comparing to hCO cultured in STEMdiff™ Neural Organoid Basal Medium 2 (hCO + STEMdiff™ NOBM, STEMCELL Technologies, 08620), the remaining organoids in each well were imaged at day 12 on a REVOLVE microscope (ECHO ...
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bioRxiv - Developmental Biology 2019Quote: Placenta cell suspensions were suspended in Iscove’s MDM with 2% fetal bovine serum and cultured using a MethoCult GF M3434 Optimum kit (STEMCELL Technologies). 10,000 cells from each sample were plated in 35-mm culture dishes (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... the iPSC were maintained in StemFlex medium and passaged 1-2 a week using ReLSR (STEMCELL Technologies, Vancouver, BC, Canada) dissociation buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... half of the medium was refreshed weekly with BrainPhys medium (BP) supplemented with 2% NeuroCult SM1 neuronal supplement (STEMCELL Technologies) and 1% penicillin/streptomycin.
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Physiology 2020Quote: ... and maintained at 37 °C with 5% CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver, Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: Primary mouse clonogenic progenitor assays or colony forming units (CFU) assays were performed by plating 5×105 unfractionated nucleated BM cells in methylcellulose M3434 (StemCell Technologies, Vancouver, Canada) in 1 ml duplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5% and 5% (v/v) of the hydrophilic concentrate BMGLS in PneumaCult™ ExPlus or PneumaCult™ ALI medium (Stemcell Technologies, Vancouver, Canada) supplemented with MycoZAP (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were collected by centrifugation at 1300 rpm for 5 minutes and 500 K cells distributed into each well of an AggreWell™800 plate (24 well) (Stem Cell Technologies) as recommended by the manufacturer.
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bioRxiv - Molecular Biology 2023Quote: ... hPSC-derived NPCs were seeded at an initial concentration of 5×105 cells/mL in STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies) and allowed to sit at 37 °C for 48 hrs ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies) or StemMACS HSC Expansion Media (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... microcapsules carrying HUES-8 cells were immersed in E8 medium for 1 h immediately after encapsulation and then transferred into E6 medium (without FGF-2 and TGF-β1, STEMCELL Technologies) for subsequent cultivation (2000 microcapsules per well) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 tumors were extracted and minced with sterile razor blades and incubated for 2 h in the presence of Collagenase (1,000 units per sample) in sterile Epicult media (Stem Cell Technology). Cells were washed with sterile filtered PBS supplemented with 1% bovine serum albumin (PBS-BSA 1% ...
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bioRxiv - Developmental Biology 2022Quote: ... hESCs were plated onto Matrigel at a density of 2×105 cells/cm2 in E8 medium containing 10 µM ROCK inhibitor (STEMCELL Technologies) and cultured overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were cut into 1-2 mm pieces and subsequently incubated in serum-free medium containing collagenase/hyaluronidase (Stem Cell Technologies) at 37 ºC ...
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bioRxiv - Cell Biology 2022Quote: CFU assays in PHZ-treated and retrovirally-infected spleen progenitors were conducted using spleen suspensions at 2 × 104 cells/mL mixed with Methocult M3434 (STEMCELL Technologies) containing Epo ...
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... 550,000 neurons were seeded in 6 well plates coated with 1 mg/mL polyLysine were cultured in conditioned Neurobasal medium supplemented with 2 mM L-glutamine and NeuroCult SM1 Neuronal supplement (STEMCELL Technologies) half renewed every 3 days with 3.4mM AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Cell Biology 2023Quote: ... 2×105 freshly isolated cells from digests of aorta or specified tissue were plated in methylcellulose (MethoCult GF M3434, StemCell Technologies), in duplicate ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow aspirates were diluted 1:1 with PBS (+ 2% FBS) and layered on a Lymphoprep gradient using SepMate tubes (STEMCELL Technologies). After centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells were counted and seeded at a density of 10.4 × 103 cells/cm2 in differentiation media (Neurobasal medium, 2% B27 supplement, 1% GlutaMAX, and 10 μM of all-trans retinoic acid [STEMCELL Technologies]). Differentiation media was replaced every other day until day 9 post-seeding ...
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bioRxiv - Neuroscience 2024Quote: ... media was removed and neural induction media (NIM; Supplementary Table 2) supplemented with small molecules 10 µM SB431542 (StemCell Technologies, #72146) and 0.1 µM LDN193189 (StemCell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... SSI and SSII media are parts of a 2-step suspension culture system with a base medium consisting of StemSpan Serum-Free Expansion Medium II (StemCell technologies), 2 mM of L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2024Quote: Whole blood was collected from 12M and 20M SM/J and BL6 mice via intracardiac puncture and diluted into an equal volume of Dulbecco’s PBS + 2% FBS (StemCell Technologies, 07905). According to the manufacturer’s specifications ...