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Citations for Stemcell Technologies :
51 - 100 of 131 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... c-Kit+) from neonatal wild-type mice were FACS sorted and cultured in MethoCultTm medium (GF M3434, Stem cell technologies) in 35mm dishes ...
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bioRxiv - Developmental Biology 2022Quote: Day 15 H9 hESC-derived enteric crestospheres were dissociated into single cells (accutase, Stemcell Technologies, 07920, 30 min, 37 °C), resuspended in ENC medium and were transferred into 384-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... sorted 250 cells/well were plated in a 6 well plate of MegaCult C Medium plus Lipids (Stem Cell Technologies) with rhEPO (recombinant human erythropoietin) ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
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bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
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bioRxiv - Cancer Biology 2024Quote: ... isolated glands were minced and digested for 16-20 hr at 37 °C in DMEM F-12 (StemCell Technologies, 36254) containing 2% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Physiology 2024Quote: ... coated with GelTrex (Fisher cat. A1413302; 1h at 37°C) and maintained in mTeSR media (Stem Cell Technologies cat. 85850). Rock inhibitor 10μM Y-27632 was added at seeding and after passages.
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bioRxiv - Immunology 2024Quote: ... Lin-c-Kitint/loFlt3+ progenitors were enriched by using an EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Stemcell Technologies), biotin-conjugated anti-Thy1.2 (53-2.1) ...
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bioRxiv - Immunology 2024Quote: ... 1x108 cells/ml were incubated with 20μl/ml EasySepTM mouse Hematopoietic Progenitor Cell Isolation Cocktail (15min, 4°C, StemCell technologies), RapidSpheres (50μl/ml ...
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bioRxiv - Cancer Biology 2024Quote: Cryopreserved PBSCs were carefully thawed and then cultured at 37°C using MethoCult™ H4435 Enriched Medium (STEMCELL Technologies Inc.), adhering to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were carefully minced with a blade and incubated overnight with gentle shaking (130 rpm) at +37 °C in Mammocult basal medium (StemCell Technologies) containing 0.2% Collagenase A (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were initially dissociated into single cell suspensions by accutase treatment (Stemcell Technologies, 07920, 30-60 min, 37 °C, 5% CO2) and then fixed and permeabilized using fixation/permeabilization buffers (Foxp3/Transcription Factor Staining Buffer Set ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Human pluripotent stem cell (hPSC)-derived cerebral organoids were grown at 37°C with 5% CO2 in STEMdiff Cerebral Organoid Kit cell culture media from STEMCELL Technologies Inc ...
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bioRxiv - Microbiology 2020Quote: ... PEG solution was aspirated and cells resuspended initially in ClonaCell-HY Medium B thereafter in ClonaCell-HY Medium C (StemCell technologies) and incubated overnight (37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... The Geltrex was allowed to set for 40 minutes in a 37°C humidified incubator with 5% CO2 before being submerged in IntestiCult media (STEMCELL technologies).
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bioRxiv - Cell Biology 2021Quote: ... Lin−Sca-1+c-Kit+CD150+CD48− (HSC) or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) populations were cultured in Myelocult M5300 (STEMCELL Technologies) with 100 µg/ml Primocin (Invivogen ...
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bioRxiv - Biochemistry 2022Quote: ... NPC cells were cultured on a poly-l-ornithine- and laminin-coated six-well plate (Falcon) at 37°C in neural expansion media (STEMCELL Technologies, 70% DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... at 5×105 LSK/mL and cultured for 2 h at 5% CO2 and 37 °C in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Developmental Biology 2021Quote: ... approximately half of the neuruloids on each coverslip were scraped off and treated for 10 min at 37 °C with Accutase (Stemcell Technologies). The remaining neuruloids were fixed for immunofluorescence analysis as a quality control ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured in a Cytomat 24 (Thermo Fischer Scientific) at 37°C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies), supplemented with 1% penicillin-streptomycin (Thermo Fischer Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... WT or Rnf5−/– Lin−Sca1+c-Kit+ cells transformed with GFP-MLL-AF9 were resuspended in methylcellulose M3234 (Stem Cell Technologies) supplemented with 6 ng/mL IL-3 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Cell Biology 2024Quote: ... were washed three times with PBS and incubated for 30 minutes at 37 °C with 60 ng/mL DNAse I (Stemcell technologies) in DNAse buffer solution (Table 1) ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were maintained in a 37°C/5% CO2 incubator and cells were fed every four days with neural maturation medium (BrainPhys plus SM1 supplement (Stemcell Technologies) supplemented with 10ng/ml of BDNF ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Neurons were grown at 37°C/5% CO2 and fed via half-media exchange every 3rd day with astrocyte conditioned BrainPhys media (StemCell Technologies) supplemented with 1% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... GBOs cultured for more than one month were dissociated into a single cell with accutase at 37 °C for 30 min and seeded into aggrewell 800 (Stemcell technologies) at a density of 9 × 105 cells/mL with 1 mL of GBO medium supplemented with 10 µM Y27632 ...
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Cancer Biology 2022Quote: ... glands were first digested overnight (∼16 hours) with gentle agitation at 37 °C in 250 μL Gentle Collagenase (Stemcell Technologies #07919) diluted in 2.25 mL of complete Basal Epicult media formulated according to manufacture instructions (Epicult Basal Medium Stemcell Technologies #05610 ...
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bioRxiv - Immunology 2022Quote: Vials of cryopreserved PBMCs were thawed at 37°C and washed in ClonaCell™-HY Medium A (STEMCELL Technologies, cat. #03801). Epstein-Barr virus (EBV ...
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bioRxiv - Genetics 2022Quote: ... Human ESCs (wild type and ADAR1 KO, gifts from Dr. C. Rice, the Rockefeller University) were maintained in complete mTeSR1 (StemCell Technologies) and the differentiated neural progenitor cells were maintained in STEMdiff Neural Progenitor Medium (StemCell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with Dulbecco’s Modified Eagle Medium (DMEM/F12) and incubated for 70 min (37°C, 5 % CO2) in STEMdiff Neural Rosette Selection Reagent (StemCell Technologies). Rosettes were lifted by gently dispensing DMEM/F12 onto the colonies ...
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bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
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bioRxiv - Developmental Biology 2023Quote: ... A single-cell suspension was obtained by incubating the dissected embryos at 37°C in a dissociation solution consisting of accutase (Stemcell Technologies) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were cultured at 37°C with 5% CO2 and fed every third day by half-media exchange using astrocyte-conditioned BrainPhys media (StemCell Technologies) supplemented with 1% FBS ...
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bioRxiv - Genomics 2024Quote: ... and 100% humidity) in E8 Flex medium (BIONi010-C and BIONi037-A) and mTeSR plus (STEMCELL Technologies, Cat. no 100-0276) (Gibco ...
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bioRxiv - Microbiology 2024Quote: WA09 embryonic stem cells (WiCell, Madison, WI, USA) were expanded and maintained at 37°C/5% CO2 in mTeSR-E8 culture medium (STEMCELL Technologies) on Nunclon cultureware coated with LDEV-free Geltrex (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Undifferentiated iPSCs and iE cells were incubated at 37°C in Gentle Cell Dissociation Reagent (Stem Cell Technologies CAT#100-0485) for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 40 µL of Matrigel containing cells were placed in each well and the plate was incubated at 37 °C for 30 min before adding 500 µL of HepatiCult Organoid Initiation Media (StemCell Technologies). The medium was changed every 2-3 days until the organoid cultures were ready for passaging (∼ 14 days) ...
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bioRxiv - Genetics 2021Quote: ... ∼4 × 105 CS iPSCs containing a c.1569G>A (p.(W523X)) mutation were treated with Accutase (working dilution 1:3 in PBS; StemCell Technologies #07922,). Cells were dissociated to generate a suspension of single cells ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: 1×105 sorted CD34+ HSPCs were seeded in for collagen-based colony-forming assays (MegaCult™-C medium with cytokines, StemCell Technologies), as previously described (Gialesaki et al. ...
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bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...