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Citations for Stemcell Technologies :
51 - 100 of 1235 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and routinely passaged 1:10 every 5 days using Gentle Cell Dissociation Reagent (StemCell Technologies).
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bioRxiv - Cell Biology 2024Quote: ... and SepMate-15 tube (StemCell technologies) by density gradient centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by cardiac specification using RPMI B27-medium containing 5 µM IWP-4 (Stem Cell Technologies) for another 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
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bioRxiv - Immunology 2024Quote: ... using a pre-coated serological pipette until the supernatant was clear of intestinal contents and mucus (10-15 times) and incubated with Gentle Cell Dissociation Reagent (STEMCELL Tech, Canada) for 15 min at room temperature (RT ...
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bioRxiv - Immunology 2021Quote: ... from healthy buffy coat donors and incubated overnight with human recombinant Interleukin 15 (IL-15) (STEMCELL Technologies). NK cells were incubated with immune complexes ...
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bioRxiv - Cell Biology 2020Quote: ... Interleukin-6 (10 ng/mL) and Heparin (5 U/mL) (all supplied by Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... coated dishes at 37°C under hypoxia conditions (10% CO2/5%O2) in mTeSR1 (StemCell Technologies). These were passaged every 4-6 days with Accutase (#A6964 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood using density gradient centrifugation at 1,200 x g for 10 min where diluted blood was layered over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) followed by harvesting of the buffy coat ...
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bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Synthetic Biology 2024Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using RelesR (STEMCELL Technologies, 05872). Identity of H1 cells was authenticated by the supplier ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coated 24-well plate in mTeSR1 (STEMCELL Technologies, cat # 85870) media supplemented with 10μM of the ROCK inhibitor Y27632 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture for knockout experiments.
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bioRxiv - Bioengineering 2020Quote: ... and cells were encapsulated in 5 mm diameter hydrogels (20 µL) and cultured for 7 days in 300 µL SFEM media (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Developmental Biology 2022Quote: ... 15 µL CloneR (Stem Cell Technologies, 5889), and 85 µL mTeSRplus.
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 15% EScult FBS (Stemcell technologies), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2020Quote: ... and 15% CloneR™ (Stemcell Technologies #05888) was added to the 50uL of Geltrex+DMEM/F12 present in the well and incubated for 1hr in a 37C incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... Human recombinant IL-15 (Stemcell Technologies, 78031) and IL-7 (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
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bioRxiv - Physiology 2024Quote: ... Cells were utilized between passages 20 and 80 and passaged every 3–5 days at a confluency of 85–100% using Accutase (STEMCELL Technologies, #07920). The cardiac differentiation protocol was performed as previously described 49 ...
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bioRxiv - Genomics 2024Quote: ... supplemented with required additional growth factors (20 ng/mL human recombinant EGF, 10 ng/mL human recombinant bFGF, 2μg/mL heparin, STEMCELL Technologies). Cultures were seeded on ultra-low attachment culture ware (Corning CLS3471 ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in 24- well plates in SFEM (Stem Cell Technologies) plus 100 ng ml−1 mTPO (Novoprotein ...
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bioRxiv - Bioengineering 2022Quote: ... and then aggregated in AggreWell 800 24-well plates (STEMCELL Technologies) at a density of 3,000,000 cells per well with 2mL of AggreWell Medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... or IL-15 (25 ng/mL; STEMCELL Technologies) for 15 min ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... H9-hESC SOX10::GFP reporter and H20961 iPSC were passaged ∼1:6 every 4-5 days by incubating them in ReLeSR (StemCell technologies) for 1 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 1×10^5 cell per ml were plated in triplicate into M3234 incomplete methylcellulose medium (StemCell Technologies) supplemented with 0.1 ng/mL GM-CSF (Peprotech) ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Neuroscience 2021Quote: ... were isolated from E13.5 pregnant CD-1 mice and cultured on poly-D-lysine (20 μg/mL) and laminin (10 μg/mL)-coated plates in NeuroCult Basal Medium containing NeuroCult Proliferation Supplement (StemCell Technologies) and 20 ng/mL EGF ...
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bioRxiv - Bioengineering 2020Quote: ... On days 8-15+ cell culture media was exchanged every 2 days with 15 mL of Cardiomyocyte Maintenance Medium (STEMCELL Technologies). On day 30 ...
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bioRxiv - Bioengineering 2020Quote: ... Wells of a 24-well AggreWell™ 400 plate (STEMCELL Technologies, #34411) were seeded with either 1:1 iEndo-mKate2:WT-eGFP cells in EBCM with 10 μM Y-27632 ...
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bioRxiv - Bioengineering 2020Quote: ... Wells of a 24-well AggreWell™ 800 plate (STEMCELL Technologies, #34815) were treated with an anti-adhesive coating of 0.2% Pluronic F127 in PBS and seeded with 1.5×106 cells/well using either WT PGP1 hiPSCs or a 1:2 ratio of iEndo:WT PGP1 hiPSCs in EBCM supplemented with 10 μM Y-27632 ...
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bioRxiv - Developmental Biology 2023Quote: ... media was switched to RSeT media after 24 hours (05978, STEMCELL Technologies). Cells were maintained in RSeT and passaged as above every 4-5 days ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... cells were grown in 24 well AggreWell 400 plates (Stemcell Technologies, 34415). Each well was washed once with 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: Commercially available microwell plates (AggreWellTM 400 24-well plate, 34415, StemCell Technologies) were used to generate retinal and thalamic spheroids (250 ...
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bioRxiv - Genomics 2023Quote: ... 20% BIT (StemCell Technologies), 40µg/mL LDL (Millipore-Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
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bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with rhIL-15 (1ng/ml, STEMCELL Technologies) at 37°C overnight ...
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bioRxiv - Genomics 2020Quote: ... prefilled with 15 mL Lymphoprep™(Stemcell technologies, # 07851), followed by a centrifugation step of 30 min at 400g (acceleration 5 ...