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Citations for Stemcell Technologies :
51 - 100 of 365 citations for 7 benzyl 2 7 diazaspiro 4.5 decane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... To make single-cell suspensions for re-plating, the spheres are chemically dissociated after 7 days of plating, using NeuroCult™ Chemical Dissociation Kit (Mouse, #05707) from Stem Cell Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: DCs were isolated from SPLNs of mice at 7 weeks of age using a mouse CD11c positive selection kit II (STEMCELL Technologies, Vancouver, BC). Fecal lysates used for stimulation were prepared by resuspending fecal samples (100 mg feces in 1ml of PBS ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... On day 3 cells were changed into a 50/50 ratio of fibroblast media and TeSR™ E-7™ Reprogramming Media (STEMCELL Technologies), and on day 5 were changed into 100% E-7™ Reprogramming Media ...
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bioRxiv - Genetics 2023Quote: ... Lentivirus media was removed after 24 hours and the cells were allowed to rest for another 24 hours before being selected for 7 days with Puromycin at 8µg/ml (73342, STEMCELL Technologies, Cambridge, MA, USA). Surviving cells were expanded ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Developmental Biology 2021Quote: ... 5% CO2 for 14 days and the CFC content was automatedly imaged after 7 and 14 days by using the StemVision imaging system (Stem Cell Technologies, Version 2.0.1.0). Additionally ...
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bioRxiv - Bioengineering 2019Quote: ... SVF was plated at a cell density of 7×106 in T75 tissue culture flasks in 15 ml of MesenCult MSC basal medium (Stem Cell Technologies, Cambridge, MA, USA) supplemented with MesenCult MSC Stimulatory Supplement ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed twice with PBS after which 4.5 ml of Accutase (STEMCELL technologies) was added ...
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bioRxiv - Developmental Biology 2021Quote: ... each containing 80µL protease mix (4.5µL/mL Accutase; Stem Cell Technologies, 07920; 4.5 µL/mL Accumax; Stem Cell Technologies, 07921 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted with an equal volume of PBS and then overlaid on 4.5 ml of Ficoll in a 15 ml SepMateTM tube (STEMCELL Technologies) and spun at 300 ×g for 10 min at 20 °C to separate plasma and cells ...
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bioRxiv - Cancer Biology 2022Quote: The premalignant mammary glands from 4.5-month-old Neu and Neu/PTx mice were dissociated using the Mammary epithelial cell enrichment kit (StemCell Technologies). The dissociated single cells were first stained with biotin-labeled anti-mouse CD31 ...
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bioRxiv - Microbiology 2021Quote: ... The rest of the whole blood layer was diluted with an equal volume of PBS and then overlaid on 4.5 ml of Ficoll in a 15 ml SepMateTM tube (STEMCELL Technologies) and spun at 1200 RPM for 10 min at 20 °C to separate peripheral blood mononuclear cells (PBMCs) ...
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bioRxiv - Microbiology 2022Quote: ... The rest of the whole blood layer was diluted with an equal volume of PBS and then overlaid on 4.5 ml of Ficoll in a 15 ml SepMateTM tube (STEMCELL Technologies) and spun at 1200 rpm for 10 min at 20 °C to separate peripheral blood mononuclear cells (PBMCs) ...
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bioRxiv - Molecular Biology 2021Quote: ... the pooled samples were diluted 1:1 with PBS+2% FBS and loaded into SepMate-15™ tubes with 4.5 mL of Lymphoprep™ with a density of 1.077g/mL (StemCell Technologies). Cells were centrifuged at 1200x g for 20min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: hESCs were dissociated into single cells using Accutase and 4.5×106 cells were seeded into AggreWell800 plates (STEMCELL Technologies, Vancouver, Canada) to form neural aggregates in STEMdiff Neural Induction Medium (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: ... was added to the blood sample before transferring to SepMate TM tubes prefilled with 4.5 mL of Lymphoprep (STEMCELL Technologies, Canada) density gradient medium ...
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bioRxiv - Physiology 2021Quote: ... was opened and either placed into individual tubes containing cold DMEM (high glucose, 4.5 g/l) before immediately being processed for cell dissociation (Stemcell Technologies, Vancouver, BC, Canada, cat# 07174). Dissociated intestinal epithelial cells were stained with antibodies for CD31 ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of 2% rat serum (Stemcell technologies) was added to each well for 15 minutes at room temperature and proceeded to further staining without a washing step.
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... 2% SM1 (StemCell Technologies), 500 µM L-Glutamine ...
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bioRxiv - Developmental Biology 2021Quote: ... Definitive endoderm cells are then differentiated into anterior ventral foregut endoderm (Stage 2) using dual SMAD inhibition for 2 days with 2 µM Dorsomorphin (STEMCELL Technologies, cat#72102) and 10 µM SB431542 (STEMCELL Technologies ...
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bioRxiv - Biophysics 2023Quote: ... with the addition of Human Recombinant Interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Bioengineering 2019Quote: ... or APEL 2 (STEMCELL Technologies) media without ROCK inhibitor and plates were placed onto LAVA illumination devices and subjected to experimental conditions ...
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bioRxiv - Neuroscience 2021Quote: ... 2 µM XAV939 (STEMCELL Technologies), 10 µM SB431542 hydrate and 2 µg/mL doxycycline hyclate (Sigma) ...
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bioRxiv - Pathology 2022Quote: ... 2 µM CHIR99021 (StemCell Technologies), and 10 ng/mL Recombinant Human BMP-7(Peprotech) ...
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bioRxiv - Cell Biology 2022Quote: ... 2% SM1 (Stem Cell Technologies), 15mM HEPES (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... IWP-2 (STEMCELL Technologies, 72124), RA (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μM SB431542 (StemCell Technologies), 1 μM DMH1 (Tocris Bioscience) ...
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bioRxiv - Bioengineering 2023Quote: ... (StemCell Technologies, #129830-38-2) in mTeSR-1 media ...
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bioRxiv - Bioengineering 2023Quote: ... (StemCell Technologies, #129830-38-2) in mTeSR-1 media ...
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bioRxiv - Neuroscience 2024Quote: ... 2% SM1 (05711, STEMCell Technologies), 1% N2 (07152 ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μg/ml Heparin (STEMCELL Technologies), 20 ng/ml bFGF (Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg/ml heparin (StemCell Technologies), epidermal growth factor (EGF ...
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bioRxiv - Neuroscience 2020Quote: ... 2% NeuroCult SM1 supplement (STEMCELL Technologies), 2 mM GlutaMax.
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/mL FGF-2 (StemCell Technology), and 5nM Rock inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 µM SB431542 (StemCell Technologies). Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... 2% SM1 (Stemcell Technologies, Vancouver, Canada), and 5 μM cytosine β-d-arabinofuranoside (AraC ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 μg/ml heparin (Stemcell Technologies), 50 units/ml penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... B27 (2%v/v, Stemcell technologies), N2 (0.5%v/v ...
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bioRxiv - Neuroscience 2024Quote: ... 2% NeuroCult SM1 (05711, Stemcell technologies), 1% N2 Supplement (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Genomics 2020Quote: ... with 2 mM L-Glutamine (Stemcell Technologies), 1x 2-Mercaptoethanol (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 µM XAV939 (StemCell Technologies, 72674). TPM was changed every day from day 3 to day 7 ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 2% SM1 (Stemcell Technologies; 05792), 5 mM L-Glutamine (Stemcell Technologies ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 2 µM Thiazovivin (StemCell Technologies) and plated in a Matrigel-coated 12-well plate at a density of 3 x 105 cells per well ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2 mM EDTA (Stemcell Technologies) and pooled for antibody staining.
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bioRxiv - Cell Biology 2022Quote: ... and 2% SM1 supplement (Stem Cell Technologies), and subsequently fed with astrocyte-conditioned BrainPhys media (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 2% SM1 (StemCell Technologies #05711), 40 μg/mL gentamycin and 2.5 μg/mL Amphotericin B medium ...
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bioRxiv - Pathology 2022Quote: ... supplemented with 2 µM Thiazovivin (StemCell Technologies) and plated in a Matrigel-coated 12-well plate at a density of 3 x 105 cells per well ...