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Citations for Stemcell Technologies :
51 - 100 of 1530 citations for 7 tert butoxycarbonyl 5 6 7 8 tetrahydro 1 2 4 triazolo 4 3 a pyrazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and interleukin-3 (IL-3; 1 ng/mL, first day only; Stemcell Technologies; Canada). Cell density was maintained between 0.7-2×106 cells/mL by daily feeding with fresh expansion medium.
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bioRxiv - Bioengineering 2019Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (Stem Cell Technologies). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Cell Biology 2020Quote: ... The hESCs and hiPSCs were maintained under 5% CO2 at 37°C with daily media change and were passaged every 5 to 7 days by ReLeSR (STEMCELL Technologies, 05872) into small clumps and replated onto the precoated culture dishes ...
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bioRxiv - Neuroscience 2019Quote: ... with medium changes every 2-3 days and passages 1-2 times per week using ReLeSR (Stem Cell Technologies). Heteroplasmy levels were regularly measured to insure they were retained across passage numbers ...
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bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
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bioRxiv - Bioengineering 2020Quote: ... After 72 hours the medium was replaced with 7 mL of neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 nM and smoothened agonist (SAG ...
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bioRxiv - Genetics 2022Quote: ... and harvested and replated on day 6 on matrix-coated tissue culture plates and cultivated for 7 days in STEMdiffTM Neural Induction Medium (Stemcell Technologies) with daily medium changes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3–5 days with ReLeSR (STEMCELL Technologies). All cells used had a normal diploid karyotype.
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml IL-6 and 10 ng/ml IL-3 (Stem Cell Technologies). Cytological analysis of blood and bone marrow smears from Inpp4b+/+ and Inpp4b-/- leukemic mice was done by classical wright-giemsa staining (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning ...
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bioRxiv - Bioengineering 2019Quote: ... and cultured at 37°C (5% CO2) for 7 days in 300 µL StemSpan™ SFEM (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 7 μM CHIR 99021(STEMCELL Technologies, Catalog #72052) for 3 days ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged every 3-4 days by dissolving the Matrigel in Gentle Cell Dissociation Reagent (GCDR - STEMCELL Technologies cat#100-0485). Organoids were broken down to smaller sizes by trituration in GCDR before washing with basal media (advanced DMEM/F12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle cell dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were passaged in small aggregates (1:7/1:10 depending on the line) using ReLeSR (StemCell Technologies). 10 μM Y-27632 (ROCK inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
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bioRxiv - Immunology 2021Quote: Female C57BL/6 mice received a single dose of mouse recombinant CSF-3 (StemCell Technologies) at 0.25mg/kg in sterile PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... IPSC colonies were picked and passaged every 5 to 7 days after transfer to the mTeSR culture system (Stemcell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... containing 300 cells and with 10ng/ml IL-3 (+IL-3 treatment) or without IL-3 (-IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates ...
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bioRxiv - Neuroscience 2022Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ...
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bioRxiv - Cell Biology 2023Quote: ... On days 4 and 6 LDM medium with added 50ng/ml BMP4 (STEMCELL Technologies) and 6ul/ml DMSO was used ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 2-3 days using accutase (Stemcell Technologies, #07920). Cells were then collected by centrifugation at 1200 rpm for 3 minutes and counted before seeding ...
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bioRxiv - Neuroscience 2020Quote: ... Medium was refreshed every 2-3 days and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies). For autophagy induction cells were treated with 200 µM Rapamycin (ChemCruz ...
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bioRxiv - Immunology 2023Quote: B cells subsets were obtained from WT or IL17 −/− naïve mice or from mice 14 d after alloantigen immunization (2 × 10 7 mitomycin C–treated BALB/c splenocytes i.p.) by negative selection (EasySep; StemCell Technologies). Purity of the B cell subset was greater than 98% ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 3-5 days using ReLeSR™ (#05872; Stem Cell Technology).
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bioRxiv - Genomics 2019Quote: ... They were passaged using 1:3 Accutase (STEMCELL Technologies).
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bioRxiv - Cell Biology 2021Quote: ... 1 μM PD03259010 and 3 μM CHIR99021 (STEMCELL Technologies) and 100 U/ml penicillin ...
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bioRxiv - Neuroscience 2021Quote: ... with 10 μM Y-23632 (only for the initial 24 hours; Wako) and subcultured every 5–7 days using ReLeSR reagent (STEMCELL Technologies).
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bioRxiv - Bioengineering 2020Quote: ... and cells were encapsulated in 5 mm diameter hydrogels (20 µL) and cultured for 7 days in 300 µL SFEM media (#09650 Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 min followed by a 3 min treatment with 5 mg/mL dispase (Stem Cell Technologies, 07913) and 1 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were maintained with media changes every 3-4 days with full neural culture media consisting of BrainPhys™ Basal Media (STEMCELL Technologies, Vancouver, BC, Canada) or Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... and passaged every 4-5 days using Gentle Cell Dissociation Reagent (StemCell Technologies). For organoid seeding ...
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bioRxiv - Molecular Biology 2023Quote: ... Every 4-5 days cells were passaged using ReLeSR reagent (Stemcell tech, 05872).
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Molecular Biology 2020Quote: ... using a working solution including 7 mL of Optiprep™ (#07820, Stemcell Technologies) with 1.4 mL of working buffer (0.25 M sucrose (200-334-9 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged by 7-min incubation with Dispase (STEMCELL Technologies, Vancouver, Canada). All hESCs have tested negative for mycoplasma.
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bioRxiv - Genomics 2020Quote: Unsorted floating cells were taken from d2-3 of the blast culture and 5×103 cells were seeded in 1 ml MethoCult (M3434 STEMCELL Technologies) per dish ...
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bioRxiv - Genetics 2019Quote: ... Cells were rinsed with PBS (5 mL) and treated with 3 mL Accutase (STEMCELL technologies) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... final concentrations of either 3 µM CHIR99021 for definitive hematopoietic differentiation or 3 µM IWP2 (Selleckchem, S7085) and 1 ng/ml activin A (Stemcell Technologies, 78001) for primitive hematopoietic differentiation were added to the media for 30 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Split NPCs 1:3 every week with accutase (STEMCELL Technologies; 7920). NPCs were further differentiated into human astrocytes by seeding dissociated single cells at 15,000 cells/cm2 density on Matrigel-coated plates in Astrocyte medium (ScienCell ...
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bioRxiv - Genomics 2020Quote: ... 3 µM CHIR99021 (Stemcell Technologies), 1 µM PD0325901 (Stemcell Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μM CHIR99021 (STEMCELL Technologies), 1 μM PD0325901 (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... + 3 µM CHIR9902 (Stemcell Technologies) + 10 µM SB-431542 (Stemcell Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Routine passaging every 4-5 days was performed using ReLeSR (Cat. # 05873; STEMCELL Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Routine passaging was performed every 4-5 days using ReLeSR (Cat. # 05873; STEMCELL Technologies) according to manufacturer’s instructions in E8/VTN or by treatment with Collagenase IV (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2, and 10 µg ml-1 Deoxyribonuclease I, Stem Cell Technologies, 07900), and mixed 70 times with a 1000 µl pipette tip ...