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Citations for Stemcell Technologies :
51 - 100 of 848 citations for 5 Pyrimidinecarbonitrile 4 6 diamino 2 methoxy 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Cancer Biology 2023Quote: ... for 5-7 days and embedded into 2 mg/ml collagen type-I (StemCell Technologies, Vancouver, Canada) gels ...
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bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
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bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO2 and passaged every 3-4 days (at ∼80% confluence) using ReLeSR (Stemcell Technologies), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... they were specified into a hiPSC-CM/stromal cell mixture using RPMI B27-containing 5 µM IWP-4 (Stem Cell Technologies) followed by another 7 days of RPMI B27+ (RPMI1640 GlutaMAX + 2% B27 supplement with insulin ...
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bioRxiv - Cancer Biology 2021Quote: IL-10 polarized human DCs (3×10^4/well) were incubated with allogeneic CD8+ T cells (1.5 × 10^5/well) in T cell media (Stem Cell Technologies) in 96 well flat bottom tissue culture plates for 4 days ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
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bioRxiv - Genomics 2020Quote: ... 6% v/v lymphoprep (STEMCELL Technologies, Canada), 1M betaine (#B0300-1VL ...
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bioRxiv - Cell Biology 2021Quote: ... 100μg/mL IL-6 (StemCell Technologies 78050), 35nM UM171 (StemCell Technologies 09655 ...
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bioRxiv - Bioengineering 2021Quote: ... 6 μM of CHIR99021 (Stemcell Technologies, 72054) was added on day 0 and left for 24h followed by medium change ...
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bioRxiv - Genomics 2022Quote: ... 6% v/v lymphoprep (STEMCELL Technologies, Canada), 1M betaine (#B0300-1VL ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Cancer Biology 2022Quote: ... the cells where count and seeded at a density of 2×10^4 cells per replicate into cytokine-supplemented methylcellulose medium (MethoCult M3434, Stemcell Technologies). After 10-14 days the colonies propagated and scored ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured as described above (see Primary NSC cell culture) until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well on Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: NSCs were cultured as described above (see “Primary NSC cell culture”) in complete activated media until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well in Matrigel (Corning ...
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bioRxiv - Bioengineering 2021Quote: ... at 5×105 LSK/mL and cultured for 2 h at 5% CO2 and 37 °C in 500 µL SFEM media (#09650, Stemcell Technologies) supplemented with 100 ng/mL SCF (#250-03 ...
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bioRxiv - Cell Biology 2022Quote: ... then gently detached with Accutase and reseeded as 2-5×104 cells/cm2 in mTeSR medium containing 10uM Y-27632 (#72304, STEMCELL Technologies) ROCK inhibitor (removed after 48h and exchanged for fresh medium without ROCK inhibitor) ...
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bioRxiv - Physiology 2022Quote: ... Proteases were neutralized with medium containing 10% FBS and resulting suspension of cells were incubated in a flask at 37°C 5% CO2 for 2 hours in PneumaCult-Ex Plus medium (Cat. 05040, Stemcell Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin to remove non-epithelial cells ...
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bioRxiv - Immunology 2023Quote: ... For 5-azacytidine (5-aza; Stem Cell Technologies) and trehalose 6,6’-dimycolate (TDM ...
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bioRxiv - Cancer Biology 2022Quote: ... and Interleukin-6 (IL6, StemCell Technologies, Cat#78050).
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bioRxiv - Cell Biology 2024Quote: ... interleukin (IL) 6 and IL 11 (StemCell Technologies). Cultures were maintained at 37°C in a 5% CO2 humidified atmosphere for 14 days with media changes on days 5 ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
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bioRxiv - Cancer Biology 2022Quote: CD8+ T-cells were aseptically isolated from the spleen and lymph nodes of C57BL/6 or FVB (6-8 weeks old) mice using the negative selection Easysep mouse CD8+ T-cell isolation kit (StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Bioengineering 2021Quote: hiPSC-derived endothelial cells (hECs) were differentiated as previously described.[85] hiPSCs were subjected to 5 μM CHIR99021 in STEMdiff APEL 2 Medium (STEMCELL Technologies, 05270) for 24 hours ...
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bioRxiv - Immunology 2021Quote: Murine T-cells were isolated from spleens of healthy 6-8-week old C57BL/6 mice using EasySep™ Mouse CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2019Quote: CD4+ T-cells were isolated from splenocytes and lymph node cells of wild type C57Bl/6 mice (6-8weeks old) using a T-cell negative selection kit (Easy Sep CD4+ T-cell enrichment kit, Stem Cell Technologies) to > 90% purity according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant IL-6 (STEMCELL Technologies) and 10 ng/ml mouse recombinant IL-3 (R&D ...
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bioRxiv - Microbiology 2020Quote: ... 6-well plates in mTeSR1 media (Stem Cell Technologies) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... with 10-6 M hydrocortisone (07904, Stem Cell Technologies), 1% penicillin streptomycin and 1ng/mL IL- 3 (R&D) ...
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bioRxiv - Bioengineering 2023Quote: ... and plated in a 6 well SmartDish (StemCell Technologies). CFU assays were quantified on day 14 using the STEMvision (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... in SmartDish meniscus-free 6-well plates (StemCell Technologies). Plates were kept in humidified chambers and colonies were imaged and manually scored after 9-14 days using the STEMvision counter (StemCell Technologies).
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 1x10-6 M hydrocortisone (StemCell Technologies, 74142), with half media exchanges twice per week.
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Cancer Biology 2023Quote: ... we enriched CD8+ T-cells from Cxcr3−/−/C57Bl/6 mice or non-transgenic C57Bl/6 mice to 80% purity using a negative selection method (Stemcell Technologies, Vancouver, Canada). Then ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of 2% rat serum (Stemcell technologies) was added to each well for 15 minutes at room temperature and proceeded to further staining without a washing step.
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bioRxiv - Neuroscience 2022Quote: The iPSCs were obtained from the National Institutes of Health Cell Repositories and maintained on Matrigel (BD)-coated 6-well plates in mTeSR Plus medium (STEMCELL Technologies) using a feeder-free culture protocol ...