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Citations for Stemcell Technologies :
901 - 950 of 1953 citations for Human Suprabasin SBSN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS and stimulated with Immunocult™ Human CD3/CD28 T cell Activator (Stemcell Technologies) (25 µL/ 106 cells ...
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bioRxiv - Microbiology 2023Quote: In vitro IL-10 assays were also performed using human Peripheral Blood Mononuclear Cells (PBMCs) (STEMCELL Technologies, Cat. # 70025.1). The assay was performed as described above ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Neuroscience 2023Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Immunology 2024Quote: ... Human whole-blood samples were diluted with 2% FBS in PBS prior to gradient centrifugation on Lymphoprep (StemCell Technologies). A percentage of the freshly isolated PBMCs was labelled with anti-human CD3 ...
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bioRxiv - Cell Biology 2024Quote: ... H1 human embryonic stem cells (WiCell, WA01) were routinely cultured as colonies in mTeSR Plus (STEMCELL Technologies, 100-0276) with 0.2% penicillin/streptomycin (BioConcept ...
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bioRxiv - Synthetic Biology 2019Quote: T cells were purified from normal donors by negative selection using the RosetteSep Human CD4+ T Cell Enrichment Cocktails according to the manufacturer’s protocols (StemCell Technologies). Primary cells were activated and expanded using Human T-activator CD3/CD28 Dynabeads (Cat # 11161D ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Cancer Biology 2022Quote: ... DNTs were enriched by depleting CD4+ and CD8+ cells using RosetteSep™ human CD4- and CD8-depletion cocktails (Stemcell Technologies). The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3 ...
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bioRxiv - Microbiology 2022Quote: ... Monocytes were isolated from whole blood by negative selection using RosetteSep human monocyte enrichment cocktail following the manufacturer’s protocol (StemCell Technologies). Using density gradient medium (Lymphoprep ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... human iPSCs were seeded on a matrigel coated dish and cultured in the STEMdiff SMADi Neural Induction medium (STEMCELL Technologies) for 7 days34 ...
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bioRxiv - Cell Biology 2020Quote: For structured illumination microscopy primary NK cells were enriched from peripheral blood of healthy human donors using RosetteSep (Stemcell Technologies). Freshly isolated cells were pre-incubated with anti-CD56 Alexa Fluor 647 (clone HCD56 ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
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bioRxiv - Cell Biology 2022Quote: hematopoietic progenitors (CD34+CD43+ for iPSC and CD34+ for primary cells) were plated in human methylcellulose medium H4434 (STEMCELL technologies), containing recombinant human cytokines and scored for the presence of colonies 14 days after.
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in a 1:1 ratio of DMEM-F12 and NeuroCult NS-A Basal Medium (Human) (Stem Cell Technologies) with 1X N-2 supplement (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Cancer Biology 2024Quote: Freshly isolated human HSPCs were plated at 200,000 cells/ml and cultured in IMDM medium supplemented with 25% BIT9500 (StemCell Technologies), 1% penicillin/streptomycin (P/S) ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... HUCB CD34+ cells harvested from Matrigel Matrix were subsequently enriched using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). Enriched CD34+ were then either used for FACS ...
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bioRxiv - Cell Biology 2023Quote: Primary Human Bronchial Epithelial Cells (HBEC) were expanded and differentiated in ALI culture following the protocol given by StemCell Technologies. Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from human donor leukopaks by gradient centrifugation on lymphoprep (cat#07861, Stemcell Technologies). Thereafter ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies # 15061) or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953 ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary NK cells were isolated from peripheral blood by negative selection using RosetteSep human NK enrichment cocktail (Stemcell Technologies, 15065). Blood was layered onto a Ficoll-Paque (Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... and the filtered cells were seeded into cell culture dishes at different densities for colony count and at 50,000 cell/cm2 for cell expansion and differentiation in Human MesenCult proliferation Medium (StemCell Technologies).
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bioRxiv - Cell Biology 2024Quote: ... human peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation of the collars (Lymphoprep, StemCell Technologies Inc, #07801). Monocytes were then enriched from the PBMC samples via the EasySep Human Monocyte Enrichment Kit (StemCell Technologies Inc ...
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bioRxiv - Immunology 2024Quote: ... FITC-conjugated mouse IgG2b anti-human CD32 Ab (clone IV.3, cat. no. 60012Fl.1, StemCell Technologies, Vancouver, BC, Canada). Mouse isotype controls APC-conjugated mouse IgG2a ...
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bioRxiv - Developmental Biology 2020Quote: ... We used the commercial kit (STEMdiff™ Cerebral Organoid Kit, StemCell Technologies 08570) with previously described modifications to increase embryoid body surface area75 ...
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bioRxiv - Cell Biology 2020Quote: ... To inhibit these pathways we used Recombinant Human Noggin Protein for BMP4 inhibition and/or XAV-939 for WNT inhibition (StemCell Technologies).
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bioRxiv - Cell Biology 2019Quote: ... Primary CD4+ T cells were purified from the blood of healthy donors by negative selection using the Human CD4+ T Cell Enrichment cocktail (StemCell Technologies). These primary cells were cultured in complete RPMI 1640 medium.
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bioRxiv - Neuroscience 2021Quote: ... integrated and inducible pure human cortical-like glutamatergic neurons (i3Ns) was completed in two weeks in BrainPhys media (STEMCELL Technologies, 05790) (Figure ...
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bioRxiv - Developmental Biology 2019Quote: Sorted cell populations were counted and plated at 5000 cells into 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Developmental Biology 2019Quote: Human iPSC line WTC11 (a kind gift from Bruce Conklin, Gladstone Institute of Cardiovascular Disease) was maintained in mTeSR1 (Stemcell Technologies) on Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were isolated from peripheral blood of donor 2395 via negative selection using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human CD4+ T cells were isolated from anonymous donor blood after apheresis by negative selection (STEMCELL Technologies #15062 and 15023). T cells were cryopreserved in RPMI-1640 (Corning #10-040-CV ...
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bioRxiv - Immunology 2021Quote: ... cells were preactivated for 5 days with IL-2 and either 1:1 aAPCs or 25 μl/ml CD3/CD28/CD2 tetramer (ImmunoCult Human CD3/CD28/CD2 T Cell Activator, STEMCELL Technologies). L cells (ATCC CRL-2648 ...
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bioRxiv - Bioengineering 2021Quote: ... and 25 ng/mL BMP4 with 50 ng/mL human recombinant Vascular Endothelial Growth Factor 165 (VEGF-165, STEMCELL Technologies, 78073) for 2 days ...
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bioRxiv - Microbiology 2020Quote: ... purified human B cells were obtained from the Human Immunology Core of the University of Pennsylvania under an Institutional Review Board-approved protocol and were isolated using the RosetteSep Human B Cell Enrichment Cocktail (StemCell Technologies) as per protocol ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... between 50-250*103 cells per 200µl of the following culture media: Human LT-HSCs (LIN−/CD38lo/CD34+/CD45RA−/CD90+) in StemSpan Serum-free expansion medium (SFEM; Stemcell Technologies) supplemented with 100ng/ml human SCF ...
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bioRxiv - Microbiology 2022Quote: ... HNAE cells were collected from healthy donors (human ethics approval GLY/01/15/HREC) and expanded using Pneumacult Ex+ (Stemcell technologies). HNAE cells were differentiated at air-liquid interface in 6.5 mm transwell with a 0.4μm polyester 156 membrane (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... 70–90% confluent human iPSC colonies were passaged chemically (Versene, Life Technologies, Cat. 15040-066 or ReLeSR, StemCell Technologies, Cat. 05872) or mechanically by StemPro® EZPassage™ Disposable Stem Cell Passaging Tool (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Peripheral blood mononuclear cells from whole blood freshly drawn from healthy human volunteers were isolated via centrifugation using RosetteSep and Lymphoprep reagents (StemCell Technologies). Monocytes were separated by adherence to tissue culture flasks ...