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Citations for Stemcell Technologies :
901 - 950 of 1811 citations for Human Suprabasin SBSN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 37°C and 5% CO2 in normoxia and in pre-equilibrated human IVC1 supplemented with 50ng/mL Insulin Growth Factor-1 (IGF1) (78078, STEMCELL Technologies) under mineral oil for 1-4h to allow for recovery ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte/stromal cell cultures were produced from HES3 human embryonic stem cells (hESCs, WiCell) maintained using mTeSR-1 (Stem Cell Technologies). hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC from a previously characterized healthy control (line 2135 or C3 in 110) were cultured in mTeSR1 media (Stemcell Technologies) on vitronectin coated dishes ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Bioengineering 2023Quote: ... ∼80% confluent human induced pluripotent stem cells (hPSCs) were dissociated into single cells with the Gentle Cell Dissociation Reagent (STEMCELL Technologies). Bioink consisting of 1.5mg/mL collagen with 5% Geltrex and high-density hPSCs (4×107 cells/mL ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
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bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... The ALDEFLUOR kit (Stemcell Technologies) was used to quantify the ALDH enzymatic activity ...
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bioRxiv - Neuroscience 2023Quote: ... StemDiff Hematopoietic Kit (Stemcell Technologies) was used to generated hematopoietic precursor cells (HPCs) ...
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bioRxiv - Immunology 2023Quote: ... Aldefluor kit (01700, Stemcell Technologies) was used for assessing RALDH activity ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF human iPSCs were seeded in a Matrigel-coated T25 flask in mTeSR1 medium (Stem Cell Technologies, #85870) with ROCK inhibitor ...
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bioRxiv - Bioengineering 2021Quote: ... CFU-E and CFU-GEMM Colony identification was performed 14 days after seeding in MethoCult media according to the Atlas of Human Hematopoietic Colonies (StemCell Technologies, 28700).
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coat (Research Blood Components) using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The crypts were embedded in 30 μl of Matrigel and cultured with Human IntestiCult™ Organoid Growth Medium (OGM) (Basal Medium and Organoid Supplement, Stemcell Technologies) supplemented with 100 IU Penicillin and 100 μg/ml Streptomycin (Corning ...
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bioRxiv - Cancer Biology 2022Quote: Differentiated human and murine H3K27M glioma cells were generated by dissociating the respective gliomaspheres into single cells with Accutase (Stemcell Technologies; # 07920) and subsequently cultured and maintained in the respective base media supplemented with 10% FBS for 14 days to generate a monolayer adherent culture ...
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bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Bioengineering 2019Quote: ... EGFP-transfected) cells were cultured on laminin-coated tissue culture flask in NeuroCult™ NS-A proliferation media for human (STEMCELL Technologies). Culture media was changed every two days ...
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bioRxiv - Immunology 2020Quote: ... Primary human airway epithelial cells isolated via bronchial brushings from consented healthy individuals were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from de-identified human leukapheresis-processed blood (New York Biologics, Inc) by centrifugation through Lymphoprep (Stem Cell Technologies.) density gradient ...
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
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bioRxiv - Neuroscience 2019Quote: ... Human splenocytes were then cultured at 1×106 cell per mL in ImmunoCult-XF T cell Expansion Medium (Stemcell Technologies, Vancouver, BC). For T cell stimulation ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: Culture of isolated human CB-derived CD34+ cells was performed in HSPC expansion media comprised of Stem Span serum-free media (StemCell Technologies #09855) supplemented with Glutamax (Invitrogen #35050061) ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from de-identified human leukapheresis-processed blood (New York Biologics, Inc) by centrifugation through Lymphoprep (Stem Cell Technologies.) density gradient ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Neuroscience 2023Quote: ... were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: CD34+ cells were enriched from de novo progenitor cells collected from each respective 3D hydrogel tissue explant culture using the EasySep Human CD34 Selection (StemCell Technologies, #17856). HUCB CD34+ cells were isolated from whole blood using Lymphoprep (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: ... Human CD4+ T cells were isolated from approximately 2 x 108 elutriated lymphocytes to approximately 90% purity by negative selection using RosetteSep human CD4+ T cell enrichment cocktail (15062; Stem Cell Technologies), using Ficoll/Hypaque (45-001-749 ...
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bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Neuroscience 2023Quote: ... plated cells were immediately supplemented with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ primary human T-cells were isolated by negative selection as per manufacturer’s instructions (Cat # 15021, 15061, RosetteSep Human T-cell Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). T-cell pellets were resuspended in dilution medium and counted using a hemocytometer with 0.4% Trypan blue viability stain (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ALDEFLUOR kit (StemCell technologies Cat #01700) was used for identification of cancer stem cells that express high levels of ALDH ...
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bioRxiv - Cancer Biology 2020Quote: The Aldefluor kit (Stem Cell Technologies) was used to characterize the ALDH activity of 4T1 cells as described in (Pearce et al ...
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bioRxiv - Molecular Biology 2021Quote: ... All kits are from STEMCELL Technologies, and differentiation was induced following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... The Aldefluor kit (Stem Cell Technologies) was used for the staining and quantification of ALDH1 activity by flow cytometry ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... The ALDEFLUOR kit (StemCell Technologies, USA) was used to assess the population with a high ALDH enzymatic activity ...