Labshake search
Citations for Stemcell Technologies :
901 - 950 of 971 citations for Human KRT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were isolated from whole blood using a density based negative selection kit according to the manufacturer’s instructions (RosetteSep Human NK Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation ...
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bioRxiv - Bioengineering 2024Quote: ... CD56+CD3- NK cells were isolated from the PBMC population using the EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ primary human T-cells were isolated by negative selection as per manufacturer’s instructions (Cat # 15021, 15061, RosetteSep Human T-cell Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). T-cell pellets were resuspended in dilution medium and counted using a hemocytometer with 0.4% Trypan blue viability stain (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: For further examination of neutrophil granulocytes we isolated granulocytes with the EasySep® Direct Human Neutrophil Isolation Kit (Stemcell Technologies, Cat# 19666), following the instructions provided by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... Human neutrophils were isolated from whole blood of healthy donors by immunomagnetic negative selection using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Isolation began from an initial blood volume of 0.5-1 ml ...
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bioRxiv - Bioengineering 2021Quote: Primary testicular cells were isolated from human testicular biopsies as previously described.[41] Testicular biopsies were transported to the lab on ice in Hypothermosol® FRS (STEMCELL Technologies, 07935) and processed within 1-2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Bioengineering 2021Quote: ... the apical channel medium was removed from the human Lung Airway Chip and the basal medium was replaced with PneumaCult-ALI medium (PneumaCult™-ALI Medium, StemCell Technologies, 05001) supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Cancer Biology 2020Quote: Clonogenic assay was realized with cells seeded into 35mm petri dish in semi-solid methylcellulose medium (Methocult™ M3231 for murin cells or Methocult™ H4230 for human cells, Stemcell Technologies). Cells were treated with the drugs for 72 hrs and centrifugated ...
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bioRxiv - Cell Biology 2020Quote: Primary human airway epithelial cells isolated via bronchial brushings from consented healthy or asthmatic subjects were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Immunology 2020Quote: ... Primary T cells were isolated from peripheral blood of healthy donors by negative selection using RosetteSept™ Human T Cell Enrichment Cocktail (STEMCELL Technologies, 15061) and activated with Dynabeads® Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Immunology 2022Quote: T cells were directly isolated from peripheral or cord blood using the RosetteSep Human T-cell Enrichment Cocktail according to the manufacturer’s protocol (Stem Cell Technologies cat#15061). T cells were cryopreserved in 90% fetal bovine serum+10% DMSO and stored in vapor phase liquid nitrogen following isolation ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2023Quote: ... Primary CD8+ and CD4+ T cells were isolated by negative selection through either RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) or RosetteSepTM Human CD4+ T Cell Enrichment Cocktail (StemCell technologies ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were immediately processed to isolate CD4+ T cells by negative selection using the EasySep Human CD4+ T Cell Isolation Cocktail (StemCell Technologies, Cat. #17952) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: Mononuclear cells from healthy human whole blood and CLL patients were isolated by density centrifugation using SepMate-50 tubes (STEMCELL Technologies, Vancouver, Canada). In short ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... assay done by culturing 100-sorted cells LIN-CD34+ CD38- CD9+ or CD9- in to MethoCult™ H4434 with human cytokine (Stem Cell Technologies) and colony formation score at day 12-14 of incubation in 37°C.
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bioRxiv - Molecular Biology 2023Quote: The human ES cell-lines (WA17 and RUES2; WiCell Research Institute, Madison, WI) were maintained in mTeSR1 Plus medium (STEMCELL Technologies, Vancouver, Canada) on plates coated with Matrigel (BD Biosciences ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: Peripheral blood was collected from Saudi patients into an EDTA-containing blood collection tube and treated with RosetteSepTM Human Progenitor Cell Basic Pre-Enrichment antibody cocktail according to the manufacturer’s instructions (Stem Cell Technologies Catalog#15226). After PBMCs separation and isolation ...
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Microbiology 2019Quote: ... Isolated PBMCs were used to isolate CD4+ T-cells by negative selection using the EasySep ™ Human CD4+ T-cells isolation kit (Stem Cell Technologies, Canada). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Neutrophils were freshly isolated directly from whole blood by negative selection immunomagnetic purification with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies; cat. no. 19666).
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Immunology 2021Quote: ... CD34+ peripheral stem cells were isolated from peripheral blood and expanded using the Complete Kit for Human Whole Blood CD34+ Cells (Stem Cell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...