Labshake search
Citations for Stemcell Technologies :
901 - 950 of 1983 citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: Frozen human bone marrow mononuclear cells were thawed and washed twice with Iscove’s Modified Dulbecco’s Medium (StemCell Technologies) containing 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
-
bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
-
bioRxiv - Cell Biology 2022Quote: ... Both epithelial and stromal cell pellets were then suspended in complete MammoCult human medium (STEMCELL Technologies, Inc., 05620) supplemented with hydrocortisone and heparin as per the manufacturer’s instructions and 1% pen/strep (Sigma ...
-
bioRxiv - Biophysics 2020Quote: ... ImmunoCult™ Human CD3/CD28/CD2 T cell activator (short T-Ac) (Stem cell Technologies, Vancouver, BC, Canada) was added to the cell culture medium (25 μL per 1 mL of cell suspension) ...
-
bioRxiv - Cancer Biology 2021Quote: ... T-cell cultures were fed with fresh 10% RPMI supplemented with human IL2 (20u/ml Stem Cell Technologies) every 2-3 days to maintain 1×106 cells/mL/mm2 ...
-
bioRxiv - Cancer Biology 2021Quote: CD14+ monocytes were selected from frozen human PBMC using anti-CD14 microbeads according to manufacturer’s instructions (Stemcell Technologies). The isolated monocytes were counted and resuspended in growth medium (Stemcell Technologies ...
-
bioRxiv - Bioengineering 2020Quote: ... and in the presence of the commercially available medium IntestiCult™ Organoid Growth Medium (Human) (STEMCELL Technologies, 06010), supplemented with the antibiotic primocin (Invivogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... and BT260 cells were cultured in NeuroCult NS-A Basal Medium (Human) with Proliferation Supplement (StemCell Technologies 05750) supplemented with EGF (20 ng/mL) ...
-
bioRxiv - Immunology 2021Quote: ... T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies cat 10990) with 6.25μl per ml culture media at 2e6cells/ml ...
-
bioRxiv - Immunology 2022Quote: Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
-
bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
-
bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by Lymphoprep and RosetteSep Human CD4+ T Cell Enrichment Cocktail (both STEMCELL Technologies). CD4-enriched cells were then CD25-enriched using CD25 MicroBeads II (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... at 2.5 x 106 cells/mL and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) at 25μL/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... Human primed PSCs cultured in mTeSR1 medium on Matrigel were passaged every five days using Dispase (STEMCell Technologies) at 1:10 split ratio.
-
bioRxiv - Cancer Biology 2023Quote: ... and UTSW63 cells were cultured in NeuroCult NS-A Basal Medium (Human) with Proliferation Supplement (StemCell Technologies 05751), supplemented with EGF (20 ng/mL ...
-
bioRxiv - Bioengineering 2023Quote: Human primary chondrocyte spheroids were formed by seeding the cells into AggreWellTM400 microwell culture plate (STEMCELL Technologies, Canada) at 300 ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies, 15023 and 15022) and EasySep Human CD4 T Cell Iso Kit (Stemcell Technologies ...
-
bioRxiv - Microbiology 2024Quote: Primary human CD4+ T cells from healthy donors were isolated from leukoreduction chambers after Trima apheresis (STEMCELL Technologies). PBMCs were isolated by Ficoll centrifugation ...
-
bioRxiv - Bioengineering 2024Quote: Primary adult blood cells were obtained from healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc ...
-
bioRxiv - Microbiology 2020Quote: ... the induced hAFECs was cultured with the SFM medium supplemented with 3 μM CHIR99021 (STEMCELL Technologies), 10 ng/ml BMP4 (Peprotech) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 3 million cells were added to one well of an Aggrewell™800 (StemCell Tech, 34815) with neural induction media ...
-
bioRxiv - Bioengineering 2023Quote: ... Stages 3-7 used basal media of DMEM supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged every 3-5 days after dissociation with ReLeSR(Stem Cell Technologies, 100-0483). The medium was supplemented with Rho-associated protein kinase (ROCK ...
-
Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: ... complete solution was removed and 3 ml 2i+Lif medium + 10uM ROCK inhibitor (Stemcell Technologies, 72304) was added ...
-
bioRxiv - Synthetic Biology 2021Quote: Primary adult blood cells were obtained from anonymous healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Pluripotent human stem cells were grown in the absence of MEF for four passages in mTeSR1 (StemCell Technologies, 05850) and seeded on Geltrex (invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... CD4+ cells were isolated by negative selection (RosetteSepTM Human CD4+ T cell Enrichment Cocktail, STEMCELL technologies, Cambridge, UK; #15023) following the manufacturer’s protocol ...
-
bioRxiv - Physiology 2019Quote: ... the colonoids were treated with a 1:1 mix of growth medium and IntestiCult-Human culture medium (StemCell Technologies). Colonoids were then dissociated into small cell aggregates (less than 40μm in size ...
-
bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coats using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... ICM T cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activators (25 μl/ml, STEMCELL Technologies). TAC T cells were stimulated with T Cell CD3/CD28 TransAct™ ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using EasySep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5x10 6 cells/mL left overnight for recovery ...
-
bioRxiv - Biophysics 2021Quote: ... 05702) and media supplemented with 20ng/ml of human recombinant epithelial growth factor (EGF - STEMCELL Technologies, Cat. No. 78006). All cells were grown in a humidified incubator atmosphere at 37°C and 5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies) or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies ...
-
bioRxiv - Microbiology 2020Quote: Human iPSC (XCL-1)98-derived neural progenitor cells (hNPCs) were purchased commercially (StemCell Technologies, Cat #70901, Lot #17080) and maintained up to ten passages according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: Human embryonic stem cell line HS181 (hPSCreg Kle001-A) was maintained on vitronectin (VTN-XL; Stem Cell Technologies, 07180) in Essential-E8 Medium (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ...
-
bioRxiv - Cell Biology 2020Quote: ... centrifugation were enriched for CD34+ cells using the RosetteSep™ Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies) as per manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... Only samples intended for incubation with SNAs were treated with RosetteSep Human Granulocyte Depletion Cocktail (StemCell Technologies, Cambridge, UK) adding 100 µL to bone marrow resuspended in 5 ml α-MEM medium for 20 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... as previously described by Sato et al.79 Organoid culture media (OCM) Human Intesticult™ (Stemcell Technologies Inc, Canada) containing 0.2% Primocin™ (Invivogen ...
-
bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation from peripheral blood leukocyte separation medium (STEMCELL Technologies). In vitro experiments were performed using human plasmacytoid dendritic cells (pDCs ...
-
bioRxiv - Immunology 2021Quote: ... 5mL of whole blood was enriched for CD4+ cells using RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). CD4+ cells were frozen in 10% DMSO/FBS and stored in liquid nitrogen ...
-
bioRxiv - Immunology 2020Quote: ... we treated CD4+ T cells with either αCD3αCD28 (ImmunoCult™ Human CD3/CD28 T Cell Activator; STEMCELL Technologies, 10971) or IFN I (1:1000 ...
-
bioRxiv - Immunology 2020Quote: ... Isolated CD3+ cells and tumor-infiltrating lymphocytes (TILs) were maintained in Human T Cells Expansion (STEMCELL Technologies, Vancouver, Canada) medium supplemented with 5% of heat-inactivated human AB serum (Omega Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured on recombinant human VTN (rhVTN) coated 60mm plates in StemFlex media (Stem Cell Technologies, cat. A3349401) and passaged using CTS EDTA Versene Solution (Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... cells from healthy donor and HSCT recipients were labeled with Rosettesep human monocyte enrichment cocktail (Stemcell Technologies, Cambridge, MA) and centrifuged through Histopaque density gradient medium (Sigma-Aldrich ...