Labshake search
Citations for Stemcell Technologies :
901 - 950 of 1323 citations for Hum ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were purified from non-adherent cells using Human Resting CD4+ T cell Isolation Kit (STEMCELL Technologies, 17962). A specific reagent to isolate resting T cells was selected to avoid the presence of pre-activated CD4+ T cells in sequencing samples ...
-
bioRxiv - Bioengineering 2020Quote: ... Initial hematopoietic lineage negative enrichment was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA), followed by collection of the Lin- Sca-1+ c-kit+ (LSK ...
-
bioRxiv - Immunology 2022Quote: ... Lm-OVA-specific CD8+ OT-I T cells were isolated from the spleen of infected mice by positive selection using the EasySep mouse CD90.1 positive selection kit (StemCell Technologies) as previously described (2 ...
-
bioRxiv - Immunology 2022Quote: ... Mature CD4 single positive (SP) thymocytes were negatively enriched using the EasySep Biotin Positive Selection Kit for depletion (STEMCELL Technologies) as previously described (51) ...
-
bioRxiv - Immunology 2022Quote: ... Het and HO mice using EasySep B cell kits by negative selection according to the manufacturer’s instructions (Stemcell Technologies, Canada). B cells were stained with anti CD21-FITC and anti CD23-PE antibodies (Biolegend ...
-
bioRxiv - Immunology 2022Quote: ... Intestinal CD4 T cells were isolated by negative selection using the EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies). Thymocyte single cell suspensions were obtained by pressing minced tissue though a 70μm strainer ...
-
bioRxiv - Immunology 2022Quote: CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies, Canada). Cd4-/- mice were adoptively co-transferred intravenously (i.v. ...
-
bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies). CD4+ T cells (4.0 × 105/well ...
-
bioRxiv - Immunology 2022Quote: ... the tetramer-bound fraction was enriched via an adapted protocol of the EasySep PE Selection Kit II (StemCell Technologies, Inc.) developed by Marc Jenkins’ lab ...
-
bioRxiv - Neuroscience 2023Quote: ... Neural progenitor cells (NPCs) were cultured as a monolayer and differentiated using a Smadi Inhibition Kit from StemCell Technologies (#08581) requiring a minimum of 3 passages to become patterned NPCs ...
-
bioRxiv - Bioengineering 2022Quote: ... Organoids were dissociated into a single-celled suspension using a modified protocol of the STEMdiff Cardiomyocyte Dissociation Kit (STEMCELL Technologies). Upon being transferred to a microcentrifuge tube ...
-
bioRxiv - Microbiology 2022Quote: ... Primary CD19+ B-cells were isolated from human PBMC using the EasySep human CD19 positive selection kit II (StemCell Technologies) and transduced using LV.B6L as described elsewhere (35) ...
-
bioRxiv - Immunology 2023Quote: ... France) were processed for the isolation of CD34+ precursor cells using the EasySep Human CD34 Positive Selection Kit (Stemcell Technologies). Isolated cells were cultured for 1-2 weeks in StemSpan Medium (Stemcell Technologies ...
-
Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Naïve CD4+ and total CD8+ T cells were isolated from the healthy donor blood using the EasySep Human Naïve CD4+ T cell or the EasySep Human CD8+ T cell isolation kit(StemCell Technologies). T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco ...
-
bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
-
bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
-
bioRxiv - Cell Biology 2023Quote: ... The timely collection of the CMs was done using STEMdiff reagent from the CM dissociation kit (STEMCELL Technologies, Vancouver, BC). STEMdiff was added to each well of hiPSC-CMs ...
-
bioRxiv - Cancer Biology 2023Quote: ... counted and used for isolation of CD34+ cells using EasySep™ human CD34 positive selection kit II (Stem cell technologies) following manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by sorting of cells using the SONY SH800 sorter or obtained with the naïve human CD4+ T cell isolation kit (StemCell Technology). For T helper cell culture ...
-
bioRxiv - Bioengineering 2023Quote: Organoids were dissociated into a single-celled suspension using a modified protocol using the STEMdiff Cardiomyocyte Dissociation Kit (STEMCELL Technologies). Upon being transferred to a microcentrifuge tube ...
-
bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were isolated using a EasySep Human CD34 Positive Selection Kit II according to the manufacturer’s protocol (STEMCELL Technologies). CD34+ HSPCs were cultured at 1×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies ...
-
bioRxiv - Immunology 2023Quote: ... CD8+ Tn or Teff cells isolated from LmOVA-infected mice by Thy1.1 positive selection kit (StemCell Technologies, Vancouver, BC, Canada) and were cultured immediately ex vivo in Seahorse XF medium (non-buffered DMEM 1640 containing 25mM glucose ...
-
bioRxiv - Immunology 2023Quote: ... and cells were counted using a hemocytometer and dead cells were removed with a magnetic bead based dead cell removal kit according to the manufacturer’s instructions (EasySep STEMCELL technologies). Following lung digestion 1/4 of the cell suspension was sorted for CD45+ CD3- CD19- Ly6G- FceRIa- CD5- NK1.1- CD127+ CD90+ ILCs and spiked back into the whole lung single cell solution ...
-
bioRxiv - Immunology 2023Quote: ... Cells from splenocytes and lymph nodes were combined to isolate naïve CD4+ T cells (CD4+CD25-CD45RB+) via EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stemcell Technologies) and the purity was assessed with FACS by labeling cells with antibodies specific to mouse CD4 (clone GK1.5) ...
-
bioRxiv - Immunology 2023Quote: ... NK cells were isolated from PBMC by depletion of non-NK cells using an NK cell isolation kit (STEMCELL Technologies). The manufacturer’s protocol was modified as follows ...
-
bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were purified by negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes ...
-
bioRxiv - Immunology 2023Quote: Monocytes were isolated by depletion of non-monocytic cells using a human monocyte enrichment kit without CD16 depletion (STEMCELL Technologies). PBMC were resuspended at 5ξ107 cells/mL and 50 μL/mL of the non-monocyte cocktail was added ...
-
bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were then sorted using an EasySep Mouse CD4+ T cell Isolation Kit (Stem Cell Technologies, #19852). The cells were incubated at 1×106 cells/mL in skew media in anti-CD28 (InVivoMab ...
-
bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
-
bioRxiv - Developmental Biology 2023Quote: ... Clones with the insertions were then tested for common chromosomal abnormalities using the hPSC Genetic Analysis Kit (STEMCELL Technologies Inc.).
-
bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
-
bioRxiv - Immunology 2023Quote: ... Splenic naïve OT II-CD4+ T cells were isolated using Mouse EasySep Naïve CD4+ T cell Isolation kit (Stem Cell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: CD11c+ cells were isolated from spleen and bone marrow cultures using EasySep Mouse CD11c Positive Selection kit (Stem Cell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
-
bioRxiv - Developmental Biology 2023Quote: ... the H1 ES cell line was differentiated to PP cells using the STEMdiff™ Pancreatic Progenitor Kit (STEMCELL Technologies, 05120) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... NK cells were purified by negative selection by using the EasySep™ Human NK cell Enrichment Kit (Stem Cell Technologies). 5×104 NK were co-cultured with NK sensitive cell line ...
-
bioRxiv - Microbiology 2023Quote: Dorsal forebrain regionalized neural organoids (RNOs) were generated using the STEMDiff™ Dorsal Forebrain Organoid Differentiation Kit (STEMCELL Technologies™) and STEMDiff™ Neural Organoid Maintenance Kit (STEMCELL Technologies™ ...
-
bioRxiv - Immunology 2023Quote: ... T cells are isolated using an EasySep™ Human T Cell Enrichment Kit as described by the manufacturer (Stemcell Technologies). For T cell activation ...
-
bioRxiv - Microbiology 2023Quote: ... Monocytes were isolated from PBMCs using EasySep Human monocyte enrichment kit without CD16 depletion (StemCell Technologies, Vancouver, British Columbia, Canada) according to the manufacturers’ instructions ...
-
bioRxiv - Genetics 2023Quote: The generated iPSCs were differentiated into three germ layers using the STEMdiffTM Trilineage Differentiation Kit (Stem Cell Technologies Catalog #05230).
-
bioRxiv - Genetics 2023Quote: The differentiation of hESCs toward beating cardiomyocyte was performed following STEMdiffTM Ventricular Cardiomyocyte Differentiation Kit (Stem Cell Technologies Catalog #05010) in accordance with the manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
-
bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated into single-cell suspension using Accutase and re-seeded onto fresh Poly-L-Ornithine / Laminin coated 12-well plates at a density of 150,000 cells in STEMdiff Neuron Maturation Kit medium (Stemcell Technologies) and medium exchanged every other day until days 14 and 21.
-
bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... Frozen cells were thawed at 37°C and treated using the ErythroClear™ Red Blood Cell Depletion Kit (STEMCELL Technologies). Cells were constructed into barcoded single-cell cDNA libraries and sequenced as described above.
-
bioRxiv - Cancer Biology 2021Quote: ... where randomly grouped and 2 x 106 naïve Ly5.2+CD8+CD44loCD62Lhi OT-I T cells [purified by FACS or using the EasySep™ murine naïve CD8+ T cell isolation kit (Stemcell Technologies)] were injected intravenously ...
-
bioRxiv - Cell Biology 2019Quote: ... at a density of 9000 cells per well in 100 μl of Embryoid Body Formation Medium (STEMdiff Cerebral organoid Kit, STEMCELL Technologies) with either addition of 10 μM Y-27632 (Tocris ...
-
bioRxiv - Cell Biology 2020Quote: ... The buffy coat was first separated by Ficoll-Paque Plus solution and the NK cells were then purified from the Peripheral Blood Mononuclear Cells (PBMCs) by negative selection using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol ...