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Citations for Stemcell Technologies :
901 - 950 of 1022 citations for 4 Triethylsilyl 3 butyn 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... when they reached high confluency and re-plated in mTeSR 1 with RHO/ROCK Pathway Inhibitor Y27632 (Stem Cell Technologies, 72302). Patient donors are identified with a code with one numeral (DEL_1 ...
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bioRxiv - Microbiology 2020Quote: ... ectoderm differentiation was performed with basal Medium (DMEM/F12, 10% knockout serum replacement (KOSR) and 1% Glutamax) supplemented with 10 μM SB431542 (STEMCELL Technologies) and 200 nM Noggin (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... larvae were tricaine-anesthetized and embedded within optically clear glass imaging cubes (Mizar Imaging) in embryo medium with 1% low melt agarose and 20% OptiPrep (Stemcell Technologies, Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... Medium was replaced every other day and cells were passaged ~1:10 onto fresh Matrigel-coated vessels using ReLeSR (StemCell Technologies) when 90% confluent.
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bioRxiv - Cancer Biology 2022Quote: ... Patient samples were cultured in RPMI with 10% FBS and 10% HS-5 conditioned media (ATCC) or SFEMII supplemented with 1x StemSpan CD34+ Expansion Media and 1 µM UM729 (StemCell Technologies).
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bioRxiv - Neuroscience 2024Quote: ... In vitro differentiation was achieved by sequential culture of the cells in the following media: Step 1 (mTeSR (Stem Cell Tech) + 80 ng/ml BMP4 (PeProtech ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells were counted and seeded at a density of 10.4 × 103 cells/cm2 in differentiation media (Neurobasal medium, 2% B27 supplement, 1% GlutaMAX, and 10 μM of all-trans retinoic acid [STEMCELL Technologies]). Differentiation media was replaced every other day until day 9 post-seeding ...
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bioRxiv - Cell Biology 2024Quote: ... Semi-solid MC3434 cell mixtures were plated at 1 mL mixture/well in a 6-well meniscus-free dish (SmartDishTM, STEMCELL Technology) using a 3cc syringe and a blunt-end 16-gauge needle (STEMCELL Technology) ...
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bioRxiv - Immunology 2024Quote: ... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
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bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Genetics 2023Quote: ... the cells were washed with DMEM/F12 and then incubated for ∼1.5 hours with 1 mL of STEMdiff Neural Rosette Selection Reagent (STEMCELL Technologies, #05832) in each well ...
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bioRxiv - Cancer Biology 2023Quote: ... enriched MDA-MB-231 cell line cells or CTCs were stained for EpCAM (1:50, VU1D9, Stemcell Technologies, Vancouver, Canada, AF488) and CD45 (1:25 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 37°C and 5% CO2 in normoxia and in pre-equilibrated human IVC1 supplemented with 50ng/mL Insulin Growth Factor-1 (IGF1) (78078, STEMCELL Technologies) under mineral oil for 1-4h to allow for recovery ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Neuroscience 2023Quote: ... were prepared using the osmotic shock protocol and burst onto freshly plasma-cleaned Si/SiO2 chips containing 5 µm diameter holes in presence of HEPES buffer (25 mM HEPES, 140 mM KCl, 1 mM DTT) supplemented with index matching 45% Optiprep(TM) (Stemcell Technologies) and 5 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... was prepared using the osmotic shock protocol and burst onto freshly plasma-cleaned Si/SiO2 chips containing 5 µm diameter holes in presence of HEPES buffer (25 mM HEPES, 140 mM KCl, 1 mM DTT) supplemented with index matching 45% Optiprep(TM) (Stemcell Technologies) and 5 mM MgCl2 70 ...
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bioRxiv - Microbiology 2023Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte/stromal cell cultures were produced from HES3 human embryonic stem cells (hESCs, WiCell) maintained using mTeSR-1 (Stem Cell Technologies). hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... was added into the dissociated CO/PCCO single cells suspension together with 1 µL Biotin selection cocktail (Annexin V, STEMCELL Technology), gently mixed and incubated at RT for 3 min ...
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... triturated with a 1000 µl pipette and dissociated in a collagenase solution with 1 U of Dispase (Stem Cell Technologies, 07923), 2 mg/ml Collagenase IV (Worthington Biochemical Corporation ...
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bioRxiv - Microbiology 2023Quote: ... Criteria included hESC stemness of at least 80% of double positive cells for both stemness markers SSEA-1 (Stem Cell Technologies) and TRA-1-60 (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... The mTeSR-1 cell medium (was replaced on a daily basis thereafter and the iPSC colonies were subcultured every seven days using 1 U/ml Dispase (Stemcell Technologies). During cultivation ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies). After 14 days ...
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bioRxiv - Bioengineering 2023Quote: ... was prepared by mixing equal volumes of 3mg/mL neutralized collagen with 10% Geltrex and 8×107 cells/mL cell suspension in mTeSR 1(STEMCELL Technologies) containing 10μM ROCK inhibitor Y27632 (Cayman) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 106 lineage negative cells/ml were transduced with 1 x 108 TU/ml of empty vector or Vegfc lentivirus in serum-free Stem-Span medium (StemCell Technologies) supplemented with 10 ng/ml IL-3 ...
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bioRxiv - Cancer Biology 2024Quote: ... and SK-ES-1 cells were grown and dissociated as above and cells were resuspended in MesenCult MSC basal media (Stemcell Technologies) supplemented with MesenCult MSC Stimulatory Supplement (Human ...
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bioRxiv - Cell Biology 2024Quote: ... For ECM concentration experiments and mechanical actuation experiments on Mechanoculture FX2 plates fibronectin coating (1 µg/mL and 10 µg/mL, in PBS StemCell Technologies) at 37°C for 1 hour were used.
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bioRxiv - Molecular Biology 2024Quote: ... from participants with HIV-1 on ART were isolated from whole blood by density gradient centrifugation using LymphoprepTM and SepMateTM tubes (StemCell Technologies), and cryopreserved ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then plated at 25 cell/cm2 on 2x Cultrex-coated plates in mTeSR-Plus media with 1× CloneR supplement (STEMCELL Technologies). Plates were incubated for 2 days before changing the media to mTeSR-Plus ...
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bioRxiv - Bioengineering 2024Quote: ... 1 × 104 cells/mL CD34+ cells were plated in 35 mm cell culture dishes in 1 mL Methocult H4230 medium (Stemcell Technologies) supplemented with 2% FBS ...
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bioRxiv - Synthetic Biology 2024Quote: H1 & H9 Pansio-1 lines targeted with different BxbI expressing modalities and pMIA10.53-copGFP donor were disassociated with accutase (STEMCELL Technologies, 07922) and resuspended in PBS ...
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bioRxiv - Cancer Biology 2024Quote: Tissue samples of ovarian tumours were cut into ∼1-2 mm3 pieces using sterile scalpels and digested with collagenase type IV (STEMCELL Technologies) for 2 hours at 37°C on a rotator ...
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bioRxiv - Cancer Biology 2021Quote: Ten thousand cells of RPMI-2650 and Detroit celllines were seeded in 24-well Ul-tra-Low Attachment Plates (Costar) containing 1 mL of MammoCult basal medium (Stem Cell Technologies) supplied with 10% MammoCult proliferation supplement ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... the corrected and patient cell lines were maintained in plates coated with Geltrex (1:50 DMEM/F12) overnight using mTeSR (Stem Cell technologies). The media was refreshed daily for iPSCs ...
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bioRxiv - Cell Biology 2021Quote: ... 100ug were added to 50K GMPs sorted the day before and cultured in StemSpan SFEMII supplemented with 1% L-Glutamine and Penicillin/Streptomycin with no cytokines (Stem cell technologies). Cells were cultured in a humidified incubator at 37°C and 5% CO2 for 12 hours and then washed twice with PBS-2%FBS with 7AAD ...
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bioRxiv - Cancer Biology 2022Quote: 1×105 sorted CD34+ HSPCs were seeded in for collagen-based colony-forming assays (MegaCult™-C medium with cytokines, StemCell Technologies), as previously described (Gialesaki et al. ...
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Neuroscience 2022Quote: ... IPSCs were then collected and spun down at 500rpm for 1-2 minutes and resuspended in 2mL of mTeSR Plus media with 20 µM ROCK inhibitor Y-27632 (Stemcell Technologies # 72304). Matrigel ...
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bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
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bioRxiv - Cancer Biology 2020Quote: ... Each fragment was placed in 1 mL of freezing media (400 μL of NeuroCult NS-A Basal medium with proliferation supplement (StemCell Technologies; #05751) containing 20 μg/mL rhEGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... mTESR™ 1 medium was replaced with STEMdiff™ Hematopoietic Supplement A medium from the STEMdiff™ Hematopoietic kit (# 05310; STEMCELL technologies). On day 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... All GSC lines and human neural stem cells were maintained as neurospheres using ultra low-adherence culture dishes and dissociated 1-2 times per week with Accutase (StemCell Technologies 07922). Primary human astrocytes were cultured adherently on plates coated with Geltrex (Life Technologies A1413202) ...
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bioRxiv - Genetics 2021Quote: ... Cells were resuspended in 1 mL pre-warmed Neuronal Expansion Media with Rock inhibitor (Y-27632 compound, Stem Cell Technologies, cat# 72304) at a final concentration of 10 μM and a cell count performed ...
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...