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Citations for Stemcell Technologies :
901 - 950 of 1049 citations for 2 Amino 3 chloro 5 nitro 6 picoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... in a humidified atmosphere of 5% CO2 at 37°C on low-attachment plates (62) and were dissociated with Accutase (StemCell Technologies) for passaging and plating.
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bioRxiv - Bioengineering 2024Quote: ... HSPCs were thawed and cultured at a concentration of 5×105 cells/ml in the “HSPC medium” containing StemSpan (STEMCELL Technologies) supplemented with penicillin/streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Biochemistry 2023Quote: Cells were counted and adjusted to a concentration of 5 x 105 cells/mL with ALDEFLUOR assay (01700; Stem Cell Technologies) buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... transduced mouse bladder urothelial or prostate epithelial organoids were liberated by dissociating Matrigel matrix with Dispase 5 U/ml (STEMCELL Technologies). Organoids were washed with PBS and resuspended in ice cold Matrigel with either 105 EBLM or UGSM and subcutaneously injected into the flanks of six-to eight-week-old male NSG (NOD-SCID-IL2Ry-null ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Cell Biology 2023Quote: Luminal or luminal progenitor cells were isolated from mouse mammary glands and sorted in 96-well plate filled with 5% FBS EpiCULT full medium (STEMCELL Technologies). 500 luminal cells or 200 luminal progenitors were then transferred to a 48-well plate previously seeded with 10,000 NIH3T3 irradiated feeder cells per well and incubated at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated overnight in media supplemented with 5 µM of the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (STEMCELL Technologies).
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bioRxiv - Microbiology 2024Quote: WA09 embryonic stem cells (WiCell, Madison, WI, USA) were expanded and maintained at 37°C/5% CO2 in mTeSR-E8 culture medium (STEMCELL Technologies) on Nunclon cultureware coated with LDEV-free Geltrex (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Basal media containing either a 1:1,000 dilution of remdesivir stock (5 mM final concentration) or the equivalent volume of DMSO vehicle was prepared in PneumaCult-ALI medium (STEMCELL Technologies) and was used to replace media in the appropriate wells of the 24-well plates containing the transwells ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC cultures were passaged every 4-5 days when the colonies reached 80% confluency using the dissociation reagent ReLeSR (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... After nucleofection cells were plated onto a 10 cm dish coated with Synthemax (5 μg/cm2) with TeSR-E8 supplemented with CloneR (StemCell Technologies). Cells were cultured to 80% confluency ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were cultured at 37°C with 5% CO2 and fed every third day by half-media exchange using astrocyte-conditioned BrainPhys media (StemCell Technologies) supplemented with 1% FBS ...
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bioRxiv - Microbiology 2024Quote: CD4+ T-cells were isolated from frozen PBMCs from 5 study participants using the EasySep Human CD4+ T-Cell Enrichment Kit (Stemcell Technologies). 2 million CD4+ T-cells were then cultured in 96-well plates in duplicate in 200 μL R10+ media supplemented with 100 U/mL IL-2 ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were passaged with Versene solution (Paneco, Russia) at a ratio of 1:15 – 1:20 every 4–5 days with ROCK-inhibitor (StemCell Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... Sigma for 2 min at room temperature and then incubated in neutralization buffer supplemented with NH4Cl (0.8%, StemCell Technologies) to remove red blood cells ...
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An in vitro neuronal model replicating the in vivo maturation and heterogeneity of perineuronal netsbioRxiv - Neuroscience 2022Quote: ... the media was replaced with BrainPhys™ medium with 2% (v/v) SM1 and antibiotic-antimycotic (StemCell Technologies #5792). From 14 DIV ...
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bioRxiv - Neuroscience 2022Quote: ... neural induction was initiated using a chemically defined neural base medium (NbM) supplemented with 2 µM Dorsomorphin (StemCell Technologies), 3 µM CHIR990021 (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured at ∼1-2 x 106mL in ImmunoCult-XF T cell expansion medium (StemCell Technologies, Canada) supplemented with Penicillin-Streptomycin (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... and were differentiated for 2 days using 100 ng/ml phorbol myristate acetate (PMA) (StemCell Technologies, Cambridge, MA, USA). To study the adhesion dynamics of sickle RBCs under shear flow and hypoxia ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged 1-2 times per week using an enzyme-free reagent (ReLeSR; Stem Cell Technologies, 05872). Cells were checked for mycoplasma contamination every two weeks ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS (+ 2%FBS) and with a red blood cell lysis buffer (0.8% NH4Cl, 0.1mM EDTA, STEMCELL technologies). Thereafter ...
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bioRxiv - Biophysics 2023Quote: ... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the maintenance well using 1-2 ml cold DMEM/F12 (Stem Cell Technologies) + 15 mM HEPES + 1x GlutaMax (1:100 35050-038 ...
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bioRxiv - Immunology 2024Quote: ... Human whole-blood samples were diluted with 2% FBS in PBS prior to gradient centrifugation on Lymphoprep (StemCell Technologies). A percentage of the freshly isolated PBMCs was labelled with anti-human CD3 ...
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bioRxiv - Cancer Biology 2024Quote: ... 12 ng/mL human-bFGF (#78006) and 2 ug/mL heparin solution 0.2% (#07980) from Stemcell Technologies (Vancouver, Canada). Neurospheres were dissociated using Accutase (#00-4555-56 ...
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bioRxiv - Cancer Biology 2024Quote: ... One million of cells were cultured in 5mL STEMdiff™ APEL™2 medium (Stem Cell Technologies, Cat# 05275) in the presence of 10µM Y-27632 (Stem Cell Technologies Cat#7230) ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... we first patterned the hiPSCs with a mesenchymal protocol as previously described.(Lam, et al., 2014) Cells were treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Bioengineering 2021Quote: ... we first directed the hiPSCs towards an intermediate mesenchymal fate using a previously described protocol,[84] wherein cells are treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Neuroscience 2021Quote: ... These cells were cultured in mTeSR™1 medium (mTeSR™1 Basal Medium with mTeSR™1 5× Supplement (Stemcell Technologies) and 1% penicillin-streptomycin (P/S ...
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bioRxiv - Molecular Biology 2022Quote: ... The differentiation was started following 24 h of seeding and proceeded through a 7-stage differentiation protocol (stages 1 – 4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific, Waltham, MA] supplemented with 10% fetal bovine serum [Stemcell Technologies, Vancouver ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were seeded at 70% confluency on dishes pre-coated with Matrigel and cultured in StemMACS iPS-Brew XF + 5 μM Y-27632 (STEMCELL Technologies 72304). On D1 ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs were cultured at 1×105–5×105 cells/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies, Vancouver, Canada) or Good Manufacturing Practice Stem Cell Growth Medium (SCGM ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Bioengineering 2023Quote: ... we washed 80-90% confluent cultures with PBS-and dissociated cultures into a single-cell suspension with 5 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell culture medium was supplemented with 1:200 Rho-associated protein kinase inhibitor (ROCKi) Y-27632 (final concentration 5 µM, STEMCELL Technologies, 72302) on the first day after passage ...
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bioRxiv - Developmental Biology 2023Quote: ... Four-month-old cortical organoids were randomly selected and transferred to a recording chamber kept at 37L°C using a heating platform and a controller (TC-324C, Warner Instruments) in 5% methyl-cellulose in BrainPhys Imaging Optimized Medium (STEMCELL Technologies, #05796). Imaging was performed using a SP-8X microscope with a multiphoton laser ...
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bioRxiv - Cell Biology 2023Quote: ... The hESCs H1 cell-lines were differentiated using the extended 7-stage protocol (stages 1–4 in adherent culture, stage 5 in AggreWell (Stemcell Technologies, #34421), and stages 6 and 7 in suspension culture).
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...
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bioRxiv - Cell Biology 2024Quote: ... on an automated cell-culture platform developed on a Hamilton Microlab STAR Liquid Handling System (Hamilton Company).2,43 Cells were maintained at 37 °C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies 85850, 05876), supplemented with 1% penicillin– streptomycin (Thermo Fisher Scientific ...