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Citations for Stemcell Technologies :
851 - 900 of 1676 citations for Mouse Butyrophilin Like Protein 2 BTNL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... All GSC lines and human neural stem cells were maintained as neurospheres using ultra low-adherence culture dishes and dissociated 1-2 times per week with Accutase (StemCell Technologies 07922). Primary human astrocytes were cultured adherently on plates coated with Geltrex (Life Technologies A1413202) ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: Whole blood was collected in heparin containing vacuutubes and 12μL of 0.5M EDTA with 1mL of PBS+2% FBS and 50μL of RBC of EasySep RBC Depletion spheres (EasySep ™ RBC Depletion Reagent: 18170, Stem Cell Technologies) were added ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
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bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
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bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured as described above (see Primary NSC cell culture) until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well on Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: NSCs were cultured as described above (see “Primary NSC cell culture”) in complete activated media until Passage 2-4 and then passaged with Accutase (STEMCELL Technologies, 07920) and seeded at a density of 5,000 cells per well in Matrigel (Corning ...
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bioRxiv - Immunology 2022Quote: The collected blood was 1:1 diluted with 2% FBS and was added to 7ml of Lymphoprep™ density gradient medium in SepMate-15 tubes (StemCell Technologies). The red blood cells ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was refreshed every 2-3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... Medium was refreshed every 2–3 days and cells were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... Whole blood was diluted 1:1 with phosphate-buffered saline (PBS) containing 2% FBS and then layered onto Lymphoprep (STEMCELL Technologies, 07851) in SepMate-50 isolation tubes (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Bioengineering 2023Quote: ... Stages 1 and 2 used basal media comprised of 1:1 mixture RPMI and DMEM F-12 supplemented with BSA and SM1 (Stem Cell Technology) or B27 (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the culture well with 1-2 ml ice cold DMEM++ (= DMEM/F12 + 15 mM HEPES (Stem Cell Technologies) + 1x GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Bioengineering 2021Quote: ... T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA) and thawed into culture as needed ...
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were frozen as a master cell bank (passage 1) and working cell bank (passage 2) in CryoStor CS10 freezer medium (STEMCELL technologies, Vancouver, Canada), supplemented with Y-27632 (5 μM ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples were extracted from differentiated NPCs or undifferentiated NPCs treated for 2 hrs with STEMdiff™ Forebrain Neuron Differentiation medium (Stem Cell Technologies) for differentiated NPCs ...
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bioRxiv - Immunology 2019Quote: ... and then purified with EasySep Human Neutrophil Kit (STEMCELL Technologies Inc. Vancouver, Canada), following manufacturers protocol ...
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bioRxiv - Biophysics 2019Quote: ... Cells were passaged by dissociation with Neurocult Chemical Dissociation Kit (Stem Cell Technologies). For immunostaining ...
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bioRxiv - Immunology 2019Quote: ... Resting human T-cell subsets were isolated using negative selection kits (Stemcell Technologies), total CD4 or CD8 T-cells were enriched using RosetteSep kits from Stemcell Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... washed with cold PBS and stained using the ALDEFLUOR Kit (STEMCELL Technologies, Canada) according to a previously described protocol [23] ...
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CUX1 and IκBζ mediate the synergistic inflammatory response to TNF and IL-17A in stromal fibroblastsbioRxiv - Immunology 2019Quote: ... Neutrophils (PMNs) purified from blood using the neutrophil isolation kit (Stem Cell Technologies) and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Biophysics 2020Quote: ... of either sex using a B-cell isolation kit (Stemcell Technologies; catalogue #19854) to deplete non-B cells ...
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bioRxiv - Immunology 2021Quote: ... iPSC were differentiated to hematopoietic progenitors using the STEMdiff Hematopoietic Kit (StemCell Technologies) over 12 days ...
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bioRxiv - Microbiology 2021Quote: ... Post-passage cells were patterned using the STEMdiff definitive endoderm kit (StemCell Technologies) for 5 days ...
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bioRxiv - Bioengineering 2021Quote: ... and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies, 17664) with EasySep™ Magnet (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2021Quote: Measurement of aldehyde dehydrogenase activity was performed using Aldefluor kit (Stem Cell Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and CD45+ cells with EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies) according to manufacturer protocols ...
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bioRxiv - Genomics 2021Quote: ... DE cultures were generated using the StemDiff Definitive Endoderm Kit (Stem Cell Technologies) as per manufacturer’s protocol for 5 days ...
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bioRxiv - Bioengineering 2021Quote: ... Negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes was performed to purify primary human T cells ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were isolated using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies).
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bioRxiv - Immunology 2022Quote: mLN DCs were isolated and purified (CD11c Positive Selection Kit II, STEMCELL Technologies) 2 days after inoculation of WT mice (T ...
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bioRxiv - Immunology 2019Quote: ... Neutrophils were isolated with the EasySep Direct Human Neutrophil Isolation Kit (Stemcell Technologies) with “The Big Easy” EasySep Magnet ...
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bioRxiv - Immunology 2020Quote: ... Elutriated monocytes were further purified with a monocyte isolation kit (Stem Cell technologies) to minimize donor-to-donor variability in contaminating cell populations ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were enriched from the same leukapheresis products using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was replaced with Mesencult ™ Adipogenic Differentiation Kit (STEMCELL TECHNOLOGIES, #05507). The adipogenic differentiation for mesenchymal stem cells was evaluated by Oil Red O staining and quantitative real-time PCR of adipogenic marker genes at 5 days of differentiation.
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated using the EasySep Human Mono Isolation Kit (STEMCELL technology, Canada) [28] ...
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bioRxiv - Immunology 2020Quote: ... prepared using the CD4 Positive Selection kit II (StemCell Technologies, Tullamarine, VIC, Australia), were used as antigen presenting cells ...
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bioRxiv - Developmental Biology 2022Quote: Cardioids were dissociated using the STEMdiff Cardiomyocyte Dissociation Kit (Stem Cell Technologies, #05025) according to the manufacturer’s protocol (incubated for 10 – 20 minutes at 37°C to thoroughly dissociate organoids ...
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bioRxiv - Biochemistry 2022Quote: ... NK cells were isolated using the EasySep NK isolation kit (StemCell Technologies 17955), and cells were cultured overnight with 0.5 μg/mL recombinant IL-2 (Biolegend 589106 ...