Labshake search
Citations for Stemcell Technologies :
851 - 900 of 1007 citations for Human CLEC4M L SIGN His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte/stromal cell cultures were produced from HES3 human embryonic stem cells (hESCs, WiCell) maintained using mTeSR-1 (Stem Cell Technologies). hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher) ...
-
bioRxiv - Neuroscience 2023Quote: Human iPSC-derived microglia (iMGL) were generated from human iPSC-derived hematopoietic progenitors using the STEMdiff Microglia Differentiation Kit and Microglia Maintenance Kit (STEMCELL Technologies) according to manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
-
bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
-
bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
-
bioRxiv - Neuroscience 2023Quote: Human iPSC from a previously characterized healthy control (line 2135 or C3 in 110) were cultured in mTeSR1 media (Stemcell Technologies) on vitronectin coated dishes ...
-
bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
-
bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were isolated from PBMCs using an EasySep Human CD4+ T Cell Isolation Kit (17952, StemCell Technologies, Canada) as per manufacturer’s instruction ...
-
bioRxiv - Immunology 2023Quote: ILC were isolated from PBMCs by negative selection using the EasySep™ Human Pan-ILC Enrichment Kit according to manufacturer’s instructions (Stemcell Technologies). ILC were sorted using FACSARIA™IIu (BD Biosciences ...
-
bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
-
bioRxiv - Bioengineering 2023Quote: ... ∼80% confluent human induced pluripotent stem cells (hPSCs) were dissociated into single cells with the Gentle Cell Dissociation Reagent (STEMCELL Technologies). Bioink consisting of 1.5mg/mL collagen with 5% Geltrex and high-density hPSCs (4×107 cells/mL ...
-
bioRxiv - Bioengineering 2023Quote: ... the cell suspension resulting from the dissociation was enriched in CD45+ cells with an EasySep Release Human CD45 Positive Selection Kit (Stemcell Technologies) following the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
-
bioRxiv - Cell Biology 2024Quote: ... mature NK cells were isolated from peripheral blood mononucleated cells with the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies). The human mesenchymal stromal cells (h-MSC ...
-
bioRxiv - Bioengineering 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights™ EasySep™ Magnets (StemCell Technologies) according to the manufacturer protocol.
-
bioRxiv - Cancer Biology 2024Quote: ... CD34+ HSPCs were obtained at >95% purity from pooled collections using a two-step Rosette-Sep/EasySep human CD34-positive selection kit (STEMCELL Technologies) according to the manufacturer’s protocols and/or FACS sorting ...
-
bioRxiv - Cell Biology 2024Quote: The CD4+ T-cell subpopulation was isolated from the CD14- fraction of PBMCs using the Easysep human CD4+ T-cell isolation kit (Stemcell Technology) as per the instructions of the manufacturer ...
-
bioRxiv - Immunology 2024Quote: ... T cells were separated with negative selection magnetic beads using EasySepTM Human T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada). Second- generation 4-1BB- costimulated TSHR CAR constructs were synthesized de novo (IDT ...
-
bioRxiv - Cell Biology 2024Quote: ... 1mM EDTA buffer and monocytes were then isolated using the magnetic EasySep Human CD14+ Selection kit (STEMCELL Technologies, Vancouver, BC, Canada) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... human MM cells were cultured at a density of 1 × 104 cells in MethoCult™ H4230 media (Stem Cell Technologies, #04230) supplemented with 15% FBS and 10% Gibco Phytohemagglutinin M form (PHA-M ...
-
bioRxiv - Microbiology 2024Quote: ... Naïve B cells were then isolated from adult blood using the EasySep Human Memory B Cell Isolation kit (STEMCELL Technologies #17864) to first remove CD27 positive memory cells ...
-
bioRxiv - Biophysics 2024Quote: NK cells were obtained from anonymous buffy coats (Karolinska Hospital, Huddinge, Sweden) by negative selection using EasySep Direct Human NK cell isolation kit (Stemcell Technology) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
-
bioRxiv - Immunology 2021Quote: ... CECs were isolated from human bone marrow aspirates or PBMC derived CECs culture using EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
-
bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
-
bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...
-
bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
-
bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
-
bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF human iPSCs were seeded in a Matrigel-coated T25 flask in mTeSR1 medium (Stem Cell Technologies, #85870) with ROCK inhibitor ...
-
bioRxiv - Bioengineering 2021Quote: ... CFU-E and CFU-GEMM Colony identification was performed 14 days after seeding in MethoCult media according to the Atlas of Human Hematopoietic Colonies (StemCell Technologies, 28700).
-
bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coat (Research Blood Components) using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
-
bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
-
bioRxiv - Cancer Biology 2021Quote: CD138+ myeloma cells were isolated from patient peripheral blood using a magnetic bead-based approach (EasySep Human CD138 Positive Selection Kit, StemCell Technologies 17877). The CD138+-enriched sample was titrated into 2.5e7 ...
-
bioRxiv - Immunology 2022Quote: ... the Negative Selection EasySep CD4+ T cell kit (17951) or RosetteSep Human CD4+ Cell Isolation Kit (15062) from Stemcell Technologies (Vancouver, Canada) according to the manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... The crypts were embedded in 30 μl of Matrigel and cultured with Human IntestiCult™ Organoid Growth Medium (OGM) (Basal Medium and Organoid Supplement, Stemcell Technologies) supplemented with 100 IU Penicillin and 100 μg/ml Streptomycin (Corning ...
-
bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
-
bioRxiv - Cancer Biology 2022Quote: Differentiated human and murine H3K27M glioma cells were generated by dissociating the respective gliomaspheres into single cells with Accutase (Stemcell Technologies; # 07920) and subsequently cultured and maintained in the respective base media supplemented with 10% FBS for 14 days to generate a monolayer adherent culture ...
-
bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: CD4 T cells were isolated from PBMC by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, 19052). Purity was typically >98%.