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Citations for Stemcell Technologies :
851 - 900 of 1447 citations for Acetamide N 2 3 dihydro 2 3 hydroxy 2 quinolinyl 1 3 dioxo 1H inden 5 yl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... with mTeSR-1 (STEMCELL Technologies). Lines used in this manuscript include ...
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bioRxiv - Neuroscience 2023Quote: ... 1% N2 Supplement (Stemcell Technologies), 1% GlutaMAX (Life Technologies)
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bioRxiv - Cancer Biology 2022Quote: ... 100nM IGF-1 (StemCell Technologies), 1x N2 Supplement (Thermofisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μM PD0325901 (STEMCELL Technologies)) and passaged as single cells onto fresh feeders using TrypLE Express (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µM CHIR99021 (StemCell Technologies), 2 µM SB431542 (StemCell Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% N2 Supplement (Stemcell Technologies), 2% B27 (Life technologies)
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bioRxiv - Neuroscience 2024Quote: ... 1% N2 Supplement (Stemcell Technologies), 2% B27 (Life technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% N2 Supplement (Stemcell Technologies), 2% B27 (Life technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% N2 Supplement (Stemcell Technologies), 1% GlutaMAX (Life Technologies)
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bioRxiv - Bioengineering 2024Quote: ... and IGF-1 (STEMCELL Technologies) were made in PBS supplemented with 0.1% BSA (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... we first patterned the hiPSCs with a mesenchymal protocol as previously described.(Lam, et al., 2014) Cells were treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Bioengineering 2021Quote: ... we first directed the hiPSCs towards an intermediate mesenchymal fate using a previously described protocol,[84] wherein cells are treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific, Waltham, MA] supplemented with 10% fetal bovine serum [Stemcell Technologies, Vancouver ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed cells were cultured at 37°C and 5%O2 in serum-free HSC media comprised of StemSpan II medium (Stem Cell Technologies) supplemented with CC100 cytokine cocktail (Stem Cell Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Cells were fed for 4-5 days until airlifted and continued to be grown at air-liquid interface (ALI) with PneumaCult ALI medium (StemCell Technologies, #05001) until fully differentiated (4 weeks)
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers were washed with PBS and then incubated with 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: E14 and RPL39L KO cells were treated for 5h at 37°C and 5% CO2 with 10μM (S)-MG132 (STEMCELL Technologies Catalog #73264) and 5μM Bafilomycin-A1 (STEMCELL Technologies Catalog #74242 ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were seeded at 70% confluency on dishes pre-coated with Matrigel and cultured in StemMACS iPS-Brew XF + 5 μM Y-27632 (STEMCELL Technologies 72304). On D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... and maintained at 37C with 5%CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Tec.) ...
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bioRxiv - Developmental Biology 2023Quote: ... Four-month-old cortical organoids were randomly selected and transferred to a recording chamber kept at 37L°C using a heating platform and a controller (TC-324C, Warner Instruments) in 5% methyl-cellulose in BrainPhys Imaging Optimized Medium (STEMCELL Technologies, #05796). Imaging was performed using a SP-8X microscope with a multiphoton laser ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... we washed 80-90% confluent cultures with PBS-and dissociated cultures into a single-cell suspension with 5 min of Accutase (StemCell Technologies Inc.) treatment at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Cell Biology 2024Quote: ... treated monolayers were washed in PBS then incubated in 500 μL dispase (5 U/ml) in Hank’s balanced salt solution (Stemcell Technologies, Cat. #07913) at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and collected in 5 mL of dissociation media consisting of Epicult-B Basal medium containing Mouse Epicult B supplement (Stemcell Technologies, 05610), 5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... on an automated cell-culture platform developed on a Hamilton Microlab STAR Liquid Handling System (Hamilton Company).2,43 Cells were maintained at 37 °C and 5% CO2 in mTeSR1 medium with and without phenol red (STEMCELL Technologies 85850, 05876), supplemented with 1% penicillin– streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Non-treated petri dishes of 100 mm diameter were coated with 5 mL of anti-adherence rinsing solution (STEMCELL Technologies, Vancouver, CA) for 5 minutes at 37°C to prevent cell adhesion ...
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bioRxiv - Synthetic Biology 2024Quote: WTC11 iPSCs90 were grown with 5% CO2 at 37°C cultured in mTeSR Plus Basal Medium (Stemcell technologies; Cat. No. 100-0276) on Greiner Cellstar plates (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: B cells from tonsils of 5 individuals were enriched using negative selection magnetic beads (EasySep Human B Cell Isolation kit, Stemcell technologies, #17954). They were then pooled to avoid batch effects ...
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bioRxiv - Genomics 2021Quote: ... onto Matrigel-coated (1 mg ml-1) 6-well plates 6 using ReLeSR (STEMCELL Technologies). Cells were differentiated to CD43+ hematopoietic progenitors using the StemCell Technologies Hematopoietic Kit (Cat #05310) ...
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bioRxiv - Molecular Biology 2020Quote: ... or β-thalassemia patients were cultured for 14-16d at 37°C and 5% CO2 in SFEM II medium (STEMCELL Technologies, Vancouver, Canada) as previously described37,38 ...
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bioRxiv - Physiology 2020Quote: ... and maintained at 37 °C with 5% CO2 with daily removal of differentiated cells and replacement of mTeSR1 media (STEMCELL Technologies, Vancouver, Canada). Cells were passaged routinely every 4 days using Dispase (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: Primary mouse clonogenic progenitor assays or colony forming units (CFU) assays were performed by plating 5×105 unfractionated nucleated BM cells in methylcellulose M3434 (StemCell Technologies, Vancouver, Canada) in 1 ml duplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5% and 5% (v/v) of the hydrophilic concentrate BMGLS in PneumaCult™ ExPlus or PneumaCult™ ALI medium (Stemcell Technologies, Vancouver, Canada) supplemented with MycoZAP (Lonza ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 and 94% N2 for 7 minutes at a rate of 20 L/min using Single Flow Meter (STEMCELL Technologies, Cat. #27311). The sealed chamber was then transferred to 37°C and cells incubated for 16 hours before harvesting for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Medium was replaced every two days and cells were split every 5 to 6 days using Gentle Dissociation reagent (STEMCELL Technologies, Vancouver, Canada). hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Mouse Intestinal Organoids were incubated in 24-well cell culture plates at 37 °C under 5% CO2 in IntestiCult Complete Organoid Medium (#06005, Stem Cell Technologies, Canada) containing CHIR-99021 (3 μM final concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were stained with CellTrackerTM Green CMFDA (5-chloromethylfluorescein diacetate) (InvitrogenTM C7025) diluted to 10 µM in serum free ImmunoCultTM NK Cell Base Medium (STEMCELL Technologies 100-0712) for 30 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... HSPCs derived from healthy patients or iPSC-derived HPCs were cultured for 14d at 37°C and 5% CO2 in SFEMII medium (STEMCELL Technologies, Vancouver, Canada) as previously described19,20 ...
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bioRxiv - Immunology 2024Quote: ... the pooled blood was carefully layered over 5 mL of Ficoll-Paque PREMIUM 1.084 (Cytiva, Uppsala, Sweden) in 15 mL SepMate-50 tubes (STEM Cell Technology, Cambridge, MA). PBMCs were collected from the interphase layer after centrifugation at 1200xg for 30 min.
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bioRxiv - Bioengineering 2024Quote: ... These cells were maintained in a 5% CO2 incubator at 37°C and were fed every 24 to 48 hours with mTeSR Plus medium (StemCell Technologies, 100-0276). The iPSCs were passaged using the StemPro EZPassage tool (Life Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10,000 cells were plated per 12-well plate well onto 5% biolaminin 521 LN and differentiation started 6 hrs post plating in TeSR-E6 (Stem Cell Technologies, 05946) with CHIR99021 (Tocris ...
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bioRxiv - Genomics 2021Quote: ... at a 1:100 dilution and in mTeSR™1 Medium (85851, STEMCELL Technologies, Vancouver, Canada) supplemented with 1% Penicillin/Streptomycin (30-002-CI ...
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bioRxiv - Immunology 2021Quote: ... medium was switched to 1:1 of MTEC and PneumaCult-Ex PLUS medium (StemCell Technologies, #05040) and fed every other day until expansion of cells to ∼80% confluent ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... 1 µM XXI (Stem Cell Technologies) or 1 µM LY411575 (Selleck Chemicals) ...
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bioRxiv - Immunology 2021Quote: ... containing 1:100 SM1 (StemCell Technologies), 1:100 NEAA (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... α-GFAP (Stemcell Technologies 60048; 1:100), α-IBA-1 (Santa Cruz Biotechnology sc-32725 ...
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bioRxiv - Cell Biology 2021Quote: ... mTeSR™1 (StemCell Technologies, 05850) media supplemented with penicillin/streptomycin (Gibco ...