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Citations for Stemcell Technologies :
851 - 900 of 1527 citations for 2' Chloro 4 5 5 dimethyl 1 3 dioxan 2 yl butyrophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Approximately 3 × 10^6 cells were placed in each well of an AggreWell 800 plate (STEMCELL Technologies # 34811) containing Stemflex medium enriched with 1X Revita supplement ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were cultured in Mammocult media supplemented with 4 μg/ml Heparin (Stem Cell Technology), 0.5 μg/ml hydrocortisone and 10% Mammocult Proliferation Supplement (Stem Cell Technology) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSCs (IMR90-4; WiCell Research Institute) were maintained with mTeSR Plus (STEMCELL Technologies, 100-0276) on dishes coated with 9-10 μg/cm2 Growth Factor Reduced Matrigel (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were passaged every 4 to 7 days with ReLeSR reagent (STEMCELL technologies catalog #100-0484) at a ratio of 1:6 per culture ...
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bioRxiv - Immunology 2020Quote: ... FcγRII or FcγRIII block was performed by addition of receptor specific mAbs (Stem cell technologies, IV.3 and 3G8) at a 1:100 dilution at the beginning of the incubation period ...
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bioRxiv - Developmental Biology 2023Quote: ... For stem cell maintenance N2B27 medium was supplemented with 3 μM CHIR99021 (Chir) (Catalog no. 72054, Stem Cell Technologies), 1 μM PD0305901 (Catalog no ...
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bioRxiv - Immunology 2024Quote: ... Then they were added to 3 ml of prewarmed (37°C) dispase [1U/mL] digestion medium (StemCell Tech 07923). The samples were incubated at 37°C for 12 min while inverting the tube every 3 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 U/ml EPO) and the indicated CM-272 concentrations into six-well plates (SmartDish™ 27370, Stemcell technologies). Cells were cultured for 8-10 days at 37 °C and 5% CO2 and colonies (defined as a collection of at least 10 cells ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 hiPSCs (passages 45–60) purchased from WiCell were maintained in mTeSR1 (85850, Stem Cell Technologies) in 6-well cell culture plates (30006 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged every 4-7 days using the enzyme-free passaging reagent ReLeSR (STEMCELL Technologies, 05872) which preserves colonies ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μM StemRegenin 1 (StemCell Technologies, Inc.), 10% charcoal stripped FBS (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were spun down (120 × g) for 3 min and resuspended in the required amount of mTeSR (Stem Cell Technologies) + Rock Inhibitor (RI) ...
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bioRxiv - Cancer Biology 2022Quote: ... MDS-L cell line was cultured in the presence of 10 ng/mL human recombinant IL-3 (Stem Cell Technologies). The MDS/AML patient samples used in this study were obtained with written informed consent under approval by the IRBs of the Albert Einstein College of Medicine ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant mouse Interleukin-3 (6 ng/ml) and recombinant human Interleukin-6 (10 ng/ml) (all from Stem Cell Technologies). 200ul fresh SFEM medium with cytokines was added on Day 5 and 8 and cells were counted on Day 5 and 10.
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bioRxiv - Cell Biology 2023Quote: ... Inflammatory macrophages from a 3-day peritoneum were isolated using the EasySep Mouse selection kit (StemCell Technologies, Vancouver, BC, Canada) with mAb F4/80 conjugated to PE according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... at a density of ±8.75 × 104 cells/cm2 or 3 × 105 cells/ml in mTeSR medium (Stem Cell Technologies, 85850) supplemented with 1:100 RevitaCell Supplement (Gibco ...
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bioRxiv - Bioengineering 2023Quote: Primary human CD3+ T cells from 3 unique donors were isolated by negative selection (T Cell Enrichment Cocktail, Stemcell Technologies) from peripheral blood and were grown in RPMI + 10% FBS + 1% PS supplemented with 50 U/mL of IL2 ...
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bioRxiv - Cell Biology 2024Quote: ... the medium was replaced by Stemfit AK02N (A + B) supplemented with CHIR99021 (3 μM, #72052; STEMCELL Technologies, Vancouver, BC, Canada) and ActivinA (10 ng/mL ...
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bioRxiv - Genomics 2021Quote: ... tissue was viably frozen by placing 4 biopsies in 500 ml of CryoStor CS10 freezing media (STEMCELL Technologies). Tubes were inverted and then placed on ice for up to 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies) for definitive endoderm induction ...
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bioRxiv - Neuroscience 2024Quote: ... hESCs were fed every two days and split every 4–7 days using ReLeSR (StemCell Technologies, 100-0483) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were cultured for 4 days using STEMdiff™ Definitive Endoderm Differentiation Kit (Cat. No. 05110, STEMCELL Technologies). Media was replaced with RPMI 1640 medium ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μM IWR-1-endo (StemCell Technologies, 72562), 5 μM SB-431542 (StemCell Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... A 3x103 cell fraction of non-transduced and transduced Lin-cells were transferred into 3 ml methylcellulose-based medium with mouse cytokines optimized for hematopoietic progenitor cell differentiation (StemCell Technologies) and plated at 1 mL/well for a CFU assay ...
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bioRxiv - Cell Biology 2020Quote: ... at 37℃ for 10 min and then were seeded on to a vitronectin-coated six-well plate at 3×105 cell/well in mTeSR1 supplemented with 5μM ROCK inhibitor (Y-27632, Stem Cell Technology) for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: CFC assays from ES and iPS cells from EB or BL-CFC were performed by plating 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies) in triplicate ...
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bioRxiv - Cancer Biology 2022Quote: CFC assays from ES and iPS cells from EB or BL-CFC were plated at 10×10^3 cells/mL into MethoCult GF (H4435 StemCell Technologies). Hematopoietic CFC was counted at day 19.
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bioRxiv - Genomics 2022Quote: ... 1000 cells of each sorted population were seeded in 1 mL of methylcellulose medium supplemented with recombinant murine SCF and IL-3 with recombinant human IL-6 and Epo (M3434, STEMCELL Technologies) in duplicate ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP positive cells with a moderate GFP signal were seeded to Matrigel-coated 96-wells in mTeSR Plus medium supplemented for 3 days with CloneR (both StemCell Technologies). After 3 days ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Cell Biology 2024Quote: ... IL-3/IL-6 and SCF cultures:3 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... 0.6 μg of a rat anti-mouse F4/80-PE antibody was incubated for 15 min at room temperature and samples were washed with 3 mL of EasySepTM buffer (ESB) (cat. no. 20144, STEMCELL Technologies), centrifuged ...
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bioRxiv - Molecular Biology 2024Quote: ... CD34+ cells were plated at a density of approximately 25,000-100,000 cells per well and spun down at 250 RPM for 3 minutes in StemSpan SFEM II media (STEMCELL Technologies, 09655). Pre-warmed media was used to resuspend the CD34+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... NPCM is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 without retinoic acid (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were cultured DIV 0 to DIV 4 in Neurocult™ Neuronal Plating Medium (Stemcell Technologies, catalog no. 05713) with SM1 neuronal supplement (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended in 4 ml Red Blood Cell Lysis buffer (Ammonium Chloride solution, Stemcell Technology, #07800) on ice for 2 min and washed with 6 mL DME/F-12 medium at 450 g for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 µM DMH-1 (STEMCELL Technologies, cat# 73632)13 ...
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bioRxiv - Genomics 2021Quote: ... diluted 1:40 in mTeSR-1 medium (StemCell Technologies). hiPSCs were passaged upon treatment with Accutase (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mg/mL DNase 1 (Stem Cell Technologies). The samples were digested for 1 hour at 37°C with constant low agitation using a thermomixer (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... Tra-1-60 (#60064, STEMCell Technologies at 1:200), stage-specific embryonic antigen-4 (SSEA-4 ...
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bioRxiv - Developmental Biology 2021Quote: ... YS suspension or sorted cells were plated in triplicate (3×103 or 100-150 cells/mL) in Methocult® M3234 (StemCell Technologies) always supplemented the cytokines listed in supplemental table 5 ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IL-15 and incubated for 3 days before actively infected cells (Thy1.2+) were isolated by magnetic positive selection (Stem Cell Technologies; 18951). Infected cells (Thy1.2+ ...
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bioRxiv - Genetics 2023Quote: ... cerebral organoids were derived from hiPSC of n=3 healthy donors using the STEMdiff™ Cerebral Organoid Kit (StemCell Technologies, #08570, #08571) according to the manufacturer’s instructions and collected on days 21 ...
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bioRxiv - Immunology 2024Quote: Vascular immune organoids (VIOs) were washed with PBS 3 times and were dissociated by 30 mins incubation of accumax (StemCell Technologies, 07921). Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-month-old and 18-month-old WT C57BL/6 mice were lineage depleted using Mammary Epithelium Enrichment Kit (Stem Cell Technologies) and resuspended in injection media (HBSS +2% FBS+1% PSA+50% Matrigel ...
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bioRxiv - Molecular Biology 2023Quote: ... NeuroDiff medium is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 (Life Technologies) ...